Production and characterization of polyhydroxybutyrate (PHB) BY Burkholderia cepacia BPT1213 using waste glycerol as carbon source

This study focused on the utilization of waste glycerol, a by-product of the biodiesel industry, used as a carbon source to grow Burkholderia cepacia BPT1213 for the production of PHB. B. cepacia BPT1213 was cultivated in minimal salt media (MSM) supplemented with waste glycerol at concentrations ra...

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Bibliographic Details
Published in:	Biocatalysis and agricultural biotechnology Vol. 41; p. 102310
Main Authors:	Drusilla Wendy, Y.B., Nor Fauziah, M.Z., Siti Baidurah, Y., Tong, W.Y., Lee, C.K.
Format:	Journal Article
Language:	English
Published:	Elsevier Ltd 01-05-2022
Subjects:	Burkholderia cepacia Fourier-transform infrared spectroscopy Nuclear magnetic resonance Polyhydroxybutyrate (PHB) Waste glycerol Polyhydroxybutyrate (PHB) Nuclear magnetic resonance Fourier-transform infrared spectroscopy Burkholderia cepacia Waste glycerol
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Summary:	This study focused on the utilization of waste glycerol, a by-product of the biodiesel industry, used as a carbon source to grow Burkholderia cepacia BPT1213 for the production of PHB. B. cepacia BPT1213 was cultivated in minimal salt media (MSM) supplemented with waste glycerol at concentrations ranging from 2% to 9% (v/v). A gradual decrease was depicted by the cell dry weight (CDW) and PHB content with increasing waste glycerol content. B. cepacia BPT1213 is able to synthesize and accumulate up to 2.41 g/L and 66.4% dry biomass and PHB yield respectively when supplemented with 2% (v/v) waste glycerol. This resulted in a total PHB concentration of 1.6 g/L. The characterization of extracted PHB was carried out by Nuclear magnetic resonance (NMR) and Fourier-transform infrared spectroscopy (FTIR) for structural composition evaluation. Both analyses showed expected signals for PHB as reported in other studies which depicted the methyl, methylene and methine groups for NMR analyses, and certain functional groups which were the hydroxyl group, carbonyl stretch and ester group for FTIR. This study demonstrated the effects of waste glycerol concentration on the end properties of PHB, which is an important aspect for its applications. •Burkholderia cepacia BPT1213 was investigated to produce polyhydroxybutyrate (PHB) using abundance and inexpensive carbon feedstocks.•The cell dry weight (CDW) and PHA content were gradually increased with decreasing of waste glycerol content used.•B. cepacia BPT1213 are able to synthesize and accumulate up to 2.41 g/L and 66.4% of dry biomass and PHA yield, respectively.•FTIR spectroscopy revealed the presence of methyl group, methylene group and methine group which are found in PHB.•Waste glycerol can serve as a suitable carbon source for the production of PHB.
ISSN:	1878-8181 1878-8181
DOI:	10.1016/j.bcab.2022.102310