Dynamics of starch formation and gene expression during grain filling and its possible influence on grain quality

Dynamics of starch formation and gene expression during grain filling and its possible influence on grain quality

In rice, grain filling is a crucial stage where asynchronous filling of the pollinated spikelet’s of the panicle occurs. It can influence both grain quality and yield. In rice grain, starch is the dominant component and contains amylose and amylopectin. Amylose content is the chief cooking quality p...

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Bibliographic Details
Published in:Scientific reports Vol. 14; no. 1; p. 6743
Main Authors: Durbha, Sanjeeva Rao, Siromani, N., Jaldhani, V., Krishnakanth, T., Thuraga, Vishnukiran, Neeraja, C. N., Subrahmanyam, D., Sundaram, R. M.
Format: Journal Article
Language:English
Published: London Nature Publishing Group UK 21-03-2024
Nature Publishing Group
Nature Portfolio
Subjects:
631/449
631/449/1734
631/449/447
Amylopectin
Amylopectin - metabolism
Amylose
Amylose - metabolism
Carbon dioxide
Crop yield
Edible Grain - metabolism
Gas exchange
Gene Expression
Grain filling
Humanities and Social Sciences
multidisciplinary
Oryza - genetics
Oryza - metabolism
Rice
RT-qPCR
Science
Science (multidisciplinary)
Starch
Starch - metabolism
Starch granule
Stomata
Stomatal conductance
Transpiration
Weight
Starch granule
Amylose
Amylopectin
Grain filling
BE2b
RT-qPCR
Rice
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Summary:In rice, grain filling is a crucial stage where asynchronous filling of the pollinated spikelet’s of the panicle occurs. It can influence both grain quality and yield. In rice grain, starch is the dominant component and contains amylose and amylopectin. Amylose content is the chief cooking quality parameter, however, rice varieties having similar amylose content varied in other parameters. Hence, in this study, a set of varieties varying in yield (04) and another set (12) of varieties that are similar in amylose content with variation in gel consistency and alkali spreading value were used. Panicles were collected at various intervals and analysed for individual grain weight and quantities of amylose and amylopectin. Gas exchange parameters were measured in varieties varying in yield. Upper branches of the panicles were collected from rice varieties having similar amylose content and were subjected to gene expression analysis with fourteen gene specific primers of starch synthesis. Results indicate that grain filling was initiated simultaneously in multiple branches. Amylose and amylopectin quantities increased with the increase in individual grain weight. However, the pattern of regression lines of amylose and amylopectin percentages with increase in individual grain weight varied among the varieties. Gas exchange parameters like photosynthetic rate, stomatal conductance, intercellular CO 2 and transpiration rate decreased with the increase in grain filling period in both good and poor yielding varieties. However, they decreased more in poor yielders. Expression of fourteen genes varied among the varieties and absence of SBE2b can be responsible for medium or soft gel consistency.
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content type line 23
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-024-57010-4