Search Results - "Sinskey, A J"

Rhodococcus opacus PD630 Heparin-Binding Hemagglutinin Homolog TadA Mediates Lipid Body Formation by MacEachran, Daniel P, Prophete, M.E, Sinskey, A.J

“…Generally, prokaryotes store carbon as polyhydroxyalkanoate, starch, or glycogen. The Gram-positive actinomycete Rhodococcus opacus strain PD630 is noteworthy…”
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Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) containing a predominant amount of 3-hydroxyvalerate by engineered Escherichia coli expressing propionate-CoA transferase by Yang, Y.-H., Brigham, C.J., Song, E., Jeon, J.-M., Rha, C.K., Sinskey, A.J.

“…Aims Of the biodegradable polyhydroxyalkanoates (PHAs), poly(hydroxybutyrate‐co‐hydroxyvalerate) (P(HB‐co‐HV)) is often considered for fabrication of…”
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Disparity between changes in mRNA abundance and enzyme activity in Corynebacterium glutamicum: implications for DNA microarray analysis by Glanemann, C, Loos, A, Gorret, N, Willis, L.B, O'Brien, X.M, Lessard, P.A, Sinskey, A.J

“…The relationship between changes in mRNA abundance and enzyme activity was determined for three genes over a span of nearly 3 h during amino acid production in…”
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Leveraging Industry-Academia Collaborations in Adaptive Biomedical Innovation by Stewart, SR, Barone, PW, Bellisario, A, Cooney, CL, Sharp, PA, Sinskey, AJ, Natesan, S, Springs, SL

“…Despite the rapid pace of biomedical innovation, research and development (R&D) productivity in the pharmaceutical industry has not improved broadly…”
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Characterization of Streptomyces MITKK-103, a newly isolated actinomycin X₂-producer by Kurosawa, K, Bui, V. P, VanEssendelft, J. L, Willis, L. B, Lessard, P. A, Ghiviriga, I, Sambandan, T. G, Rha, C. K, Sinskey, A. J

“…A new actinomycete strain designated MITKK-103 was isolated from the soil of a flowerpot using a humic acid agar medium. The newly isolated strain was able to…”
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Apoptosis in batch cultures of Chinese Hamster Ovary cells by Goswami, J., Sinskey, A. J., Steller, H., Stephanopoulos, G. N., Wang, D. I. C.

“…One of the main problems in the culture of Chinese Hamster Ovary (CHO) cells continues to be the inability to maintain the viability of the cultures over an…”
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Overexpression and Purification of the Soluble Polyhydroxyalkanoate Synthase from Alcaligenes eutrophus: Evidence for a Required Posttranslational Modification for Catalytic Activity by Gerngross, T. U, Snell, K. D, Peoples, O. P, Sinskey, A. J, Csuhai, E, Masamune, S, Stubbe, J

“…Polyhydroxyalkanoate (PHA) synthase has been expressed in Escherichia coli by reengineering the 5'-end of the wild-type (wt) gene and subsequent transformation…”
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Oxidized Redox State of Glutathione in the Endoplasmic Reticulum by Hwang, Christopher, Sinskey, Anthony J., Lodish, Harvey F.

“…The redox state of the endoplasmic reticulum (ER) was measured with the peptide N-Ace-tyl-Asn-Tyr-Thr-Cys-NH$_2$. The peptide diffused across cellular…”
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Poly-beta-hydroxybutyrate (PHB) biosynthesis in Alcaligenes eutrophus H16. Identification and characterization of the PHB polymerase gene (phbC) by PEOPLES, O. P, SINSKEY, A. J

“…The phbC gene encoding the third enzyme of the poly-beta-hydroxybutyrate biosynthetic pathway, poly-beta-hydroxybutyrate polymerase, in Alcaligenes eutrophus…”
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Expression of the Escherichia coli Catabolic Threonine Dehydratase in Corynebacterium glutamicum and Its Effect on Isoleucine Production by GUILLOUET, S, RODAL, A. A, AN, G.-H, LESSARD, P. A, SINSKEY, A. J

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New Insight into the Role of the PhaP Phasin of Ralstonia eutropha in Promoting Synthesis of Polyhydroxybutyrate by York, G M, Stubbe, J, Sinskey, A J

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Isolation and characterization of indene bioconversion genes from Rhodococcus strain I24 by TREADWAY, S. L, YANAGIMACHI, K. S, LANKENAU, E, LESSARD, P. A, STEPHANOPOULOS, G, SINSKEY, A. J

“…Rhodococcus strain 124 is able to convert indene into indandiol via the actions of at least two dioxygenase systems and a putative monooxygenase system. We…”
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Accumulation of the PhaP Phasin of Ralstonia eutropha Is Dependent on Production of Polyhydroxybutyrate in Cells by York, G M, Junker, B H, Stubbe, J A, Sinskey, A J

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Poly-beta-hydroxybutyrate biosynthesis in Alcaligenes eutrophus H16. Characterization of the genes encoding beta-ketothiolase and acetoacetyl-CoA reductase by PEOPLES, O. P, SINSKEY, A. J

“…The poly-beta-hydroxybutyrate biosynthetic thiolase gene from Zoogloea ramigera was used as a hybridization probe to screen restriction digests of Alcaligenes…”
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Mechanistic Studies on Class I Polyhydroxybutyrate (PHB) Synthase from Ralstonia eutropha:  Class I and III Synthases Share a Similar Catalytic Mechanism by Jia, Yong, Yuan, Wei, Wodzinska, Jola, Park, Chung, Sinskey, Anthony J, Stubbe, JoAnne

“…The Class I and III polyhydroxybutyrate (PHB) synthases from Ralstonia eutropha and Chromatium vinosum, respectively, catalyze the polymerization of…”
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Microparticles prepared from biodegradable polyhydroxyalkanoates as matrix for encapsulation of cytostatic drug by Murueva, A. V., Shishatskaya, E. I., Kuzmina, A. M., Volova, T. G., Sinskey, A. J.

“…Microparticles made from degradable polyhydroxyalkanoates of different chemical compositions a homopolymer of 3-hydroxybutyric acid, copolymers of…”
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Metabolic and physiological studies of Corynebacterium glutamicum mutants by Park, S. M., Sinskey, A. J., Stephanopoulos, Gregory

“…The physiology and central carbon metabolism of Corynebacterium glutamicum was investigated through the study of specific disruption mutants. Mutants deficient…”
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Engineering an indene bioconversion process for the production of cis-aminoindanol: a model system for the production of chiral synthons by O'BRIEN, X. M, PARKER, J. A, LESSARD, P. A, SINSKEY, A. J

“…Cis-aminoindanol, a key chiral precursor to the HIV protease inhibitor CRIXIVAN, can be derived from indene oxidation products of (2R) stereochemistry. A…”
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PHA synthase activity controls the molecular weight and polydispersity of polyhydroxybutyrate in vivo by Sim, Sang Jun, Snell, Kristi D, Hogan, Scott A, Stubbe, JoAnne, Rha, Chokyun, Sinskey, Anthony J

“…A synthetic operon for polyhydroxyalkanoate (PHA) biosynthesis designed to yield high levels of PHA synthase activity in vivo was constructed by positioning a…”
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Immunocytochemical analysis of poly-beta-hydroxybutyrate (PHB) synthase in Alcaligenes eutrophus H16: localization of the synthase enzyme at the surface of PHB granules by GERNGROSS, T. U, REILLY, P, STUBBE, J, SINSKEY, A. J, PEOPLES, O. P

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