Search Results - "Sinitsyn, Arkady P"
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Engineering Robust Cellulases for Tailored Lignocellulosic Degradation Cocktails
Published in International journal of molecular sciences (26-02-2020)“…Lignocellulosic biomass is a most promising feedstock in the production of second-generation biofuels. Efficient degradation of lignocellulosic biomass…”
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Protein surface engineering of endoglucanase Penicillium verruculosum for improvement in thermostability and stability in the presence of 1-butyl-3-methylimidazolium chloride ionic liquid
Published in Bioresource technology (01-01-2020)“…[Display omitted] •Protein surface engineering was performed to improve the enzyme properties.•Half-life time was improved in 1.3–1.6 times at 70 °C and…”
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N-linked glycosylation of recombinant cellobiohydrolase I (Cel7A) from Penicillium verruculosum and its effect on the enzyme activity
Published in Biotechnology and bioengineering (01-02-2016)“…ABSTRACT Cellobiohydrolase I from Penicillium verruculosum (PvCel7A) has four potential N‐glycosylation sites at its catalytic module: Asn45, Asn194, Asn388,…”
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Comparison of Two Methods for Assaying Reducing Sugars in the Determination of Carbohydrase Activities
Published in International Journal of Analytical Chemistry (01-01-2011)“…The Nelson-Somogyi (NS) and 3,5-dinitrosalicylic acid (DNS) assays for reducing sugars are widely used in measurements of carbohydrase activities against…”
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Design of highly efficient cellulase mixtures for enzymatic hydrolysis of cellulose
Published in Biotechnology and bioengineering (01-08-2007)“…An extremely highly active cellobiohydrolase (CBH IIb or Cel6B) was isolated from Chrysosporium lucknowense UV18‐25 culture filtrate. The CBH IIb demonstrated…”
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Using an Inducible Promoter of a Gene Encoding Penicillium verruculosum Glucoamylase for Production of Enzyme Preparations with Enhanced Cellulase Performance
Published in PloS one (20-01-2017)“…Penicillium verruculosum is an efficient producer of highly active cellulase multienzyme system. One of the approaches for enhancing cellulase performance in…”
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Comparative Study of the Convertibility of Pretreated Miscanthus Straw Using Enzyme Preparations Produced by Different Recombinant Strains of Penicillium verruculosum
Published in Agronomy (Basel) (01-03-2024)“…Non-edible cellulosic biomass from perennial herbaceous plants is a promising and abundant feedstock for replacing slow-growing woody plants used in…”
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Engineering the pH-optimum of activity of the GH12 family endoglucanase by site-directed mutagenesis
Published in Biochimie (01-09-2013)“…Endo-1,4-β-glucanase from Penicillium verruculosum (PvEGIII) belongs to family 12 of glycoside hydrolases (GH12). Analysis of the enzyme 3D model structure…”
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Enzymatic Hydrolysis of Kraft and Sulfite Pulps: What Is the Best Cellulosic Substrate for Industrial Saccharification?
Published in Fermentation (Basel) (01-10-2023)“…Sulfite and kraft pulping are two principal methods of industrial delignification of wood. In recent decades, those have been considered as possibilities to…”
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Effect of Novel Penicillium verruculosum Enzyme Preparations on the Saccharification of Acid- and Alkali-Pretreated Agro-Industrial Residues
Published in Agronomy (Basel) (01-09-2020)“…This study aimed at evaluating different enzyme combinations in the saccharification of sugarcane bagasse (SCB), soybean husks (SBH) and oil palm empty fruit…”
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Comparative Study of the Convertibility of Agricultural Residues and Other Cellulose-Containing Materials in Hydrolysis with Penicillium verruculosum Cellulase Complex
Published in Agronomy (Basel) (01-11-2020)“…Non-edible cellulose-containing biomass is a promising and abundant feedstock for simple sugar production. This study presents the results of different…”
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Expression and Refolding of the Plant Chitinase From Drosera capensis for Applications as a Sustainable and Integrated Pest Management
Published in Frontiers in bioengineering and biotechnology (21-09-2021)“…Recently, the study of chitinases has become an important target of numerous research projects due to their potential for applications, such as biocontrol pest…”
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Cloning, purification and study of recombinant GH3 family β-glucosidase from Penicillium verruculosum
Published in Biochimie (01-01-2020)“…A novel bgl1 gene, encoding GH3 family β-glucosidase from Penicillium verruculosum (PvBGL), was cloned and heterologously expressed in P. canescens RN3-11-7…”
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Purification and characterization of two forms of the homologously expressed lytic polysaccharide monooxygenase (PvLPMO9A) from Penicillium verruculosum
Published in Biochimica et biophysica acta. Proteins and proteomics (01-01-2020)“…Two forms of C1/C4-oxidizing lytic polysaccharide monooxygenase (PvLPMO9A) from Penicillium verruculosum (Talaromyces verruculosus) homologously expressed in…”
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The role of intracellular β-glucosidase in cellulolytic response induction in filamentous fungus Penicillium verruculosum
Published in Research in microbiology (01-05-2024)“…In this study, CRISPR/Cas9 genome editing was used to knockout the bgl2 gene encoding intracellular β-glucosidase filamentous fungus Penicillium verruculosum…”
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An engineered cellobiohydrolase I for sustainable degradation of lignocellulosic biomass
Published in Biotechnology and bioengineering (01-10-2021)“…This study provides computational‐assisted engineering of the cellobiohydrolase I (CBH‐I) from Penicillium verruculosum with simultaneous enhanced…”
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Combination of 3‑O‑Levulinoyl and 6‑O‑Trifluorobenzoyl Groups Ensures α‑Selectivity in Glucosylations: Synthesis of the Oligosaccharides Related to Aspergillus fumigatus α‑(1 → 3)‑d‑Glucan
Published in Journal of organic chemistry (01-09-2023)“…Stereospecific α-glucosylation of primary and secondary OH-group at carbohydrate acceptors is achieved using glucosyl N-phenyl-trifluoroacetimidate (PTFAI)…”
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Designing enzyme cocktails from Penicillium and Aspergillus species for the enhanced saccharification of agro-industrial wastes
Published in Bioresource technology (01-06-2021)“…•Novel enzyme cocktails from Penicillium and Aspergillus species were developed.•Native and recombinant basic cellulases and auxiliary enzymes were…”
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Rational design and structure insights for thermostability improvement of Penicillium verruculosum Cel7A cellobiohydrolase
Published in Biochimie (01-09-2020)“…Thermostability is a fundamental characteristic of enzymes that is of high importance for industrial implementation of enzymatic catalysis. Cellobiohydrolases…”
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Properties of a recombinant GH49 family dextranase heterologously expressed in two recipient strains of Penicillium species
Published in Biochimie (01-02-2019)“…The dexA gene encoding Penicillium funiculosum dextranase (GenBank accession MH581385) belonging to family 49 of glycoside hydrolases (GH49) was cloned and…”
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