SHP-1 localization to the activating immune synapse promotes NK cell tolerance in MHC class I deficiency

Natural killer (NK) cells recognize virally infected cells and tumors. NK cell function depends on balanced signaling from activating receptors, recognizing products from tumors or viruses, and inhibitory receptors (such as KIR/Ly49), which recognize major histocompatibility complex class I (MHC-I)...

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Bibliographic Details
Published in:	Science signaling Vol. 16; no. 780; p. eabq0752
Main Authors:	Schmied, Laurent, Luu, Thuy T, Søndergaard, Jonas N, Hald, Sophia H, Meinke, Stephan, Mohammad, Dara K, Singh, Sunitha B, Mayer, Corinna, Perinetti Casoni, Giovanna, Chrobok, Michael, Schlums, Heinrich, Rota, Giorgia, Truong, Hieu M, Westerberg, Lisa S, Guarda, Greta, Alici, Evren, Wagner, Arnika K, Kadri, Nadir, Bryceson, Yenan T, Saeed, Mezida B, Höglund, Petter
Format:	Journal Article
Language:	English
Published:	United States 11-04-2023
Subjects:	Animals Antigens, Ly - metabolism Histocompatibility Antigens Class I - metabolism Killer Cells, Natural Mice Receptors, NK Cell Lectin-Like - metabolism Synapses - metabolism
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Summary:	Natural killer (NK) cells recognize virally infected cells and tumors. NK cell function depends on balanced signaling from activating receptors, recognizing products from tumors or viruses, and inhibitory receptors (such as KIR/Ly49), which recognize major histocompatibility complex class I (MHC-I) molecules. KIR/Ly49 signaling preserves tolerance to self but also conveys reactivity toward MHC-I-low target cells in a process known as NK cell education. Here, we found that NK cell tolerance and education were determined by the subcellular localization of the tyrosine phosphatase SHP-1. In mice lacking MHC-I molecules, uneducated, self-tolerant Ly49A NK cells showed accumulation of SHP-1 in the activating immune synapse, where it colocalized with F-actin and the signaling adaptor protein SLP-76. Education of Ly49A NK cells by the MHC-I molecule H2D led to reduced synaptic accumulation of SHP-1, accompanied by augmented signaling from activating receptors. Education was also linked to reduced transcription of , which encodes SHP-1. Moreover, synaptic SHP-1 accumulation was reduced in NK cells carrying the H2D -educated receptor Ly49G2 but not in those carrying the noneducating receptor Ly49I. Colocalization of Ly49A and SHP-1 outside of the synapse was more frequent in educated compared with uneducated NK cells, suggesting a role for Ly49A in preventing synaptic SHP-1 accumulation in NK cell education. Thus, distinct patterning of SHP-1 in the activating NK cell synapse may determine NK cell tolerance.
ISSN:	1937-9145
DOI:	10.1126/scisignal.abq0752