Screening of Tomato Genotypes Against Root-Knot Nematode and Validation of Mi 1 Gene Linked Markers

Screening of Tomato Genotypes Against Root-Knot Nematode and Validation of Mi 1 Gene Linked Markers

In India, tomato ( Solanum lycopersicum L.) suffers yield loss between 11 and 35 % due to root-knot nematode (RKN) ( Meloidogyne  spp.) infestation. Mi 1 gene in tomato confers resistance to the three most damaging RKN species viz, Meloidogyne incognita, M. arenaria and M. javanica. The loss can be...

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Bibliographic Details
Published in:National Academy of Sciences, India. Proceedings. Section B. Biological Sciences Vol. 88; no. 1; pp. 65 - 72
Main Authors: Reddy, Yerasu Suresh, Sellaperumal, C., Prasanna, H. C., Yadav, Akhilesh, Kashyap, Sarvesh P., Singh, Satyedra, Rai, Nagender, Singh, Major, Singh, B.
Format: Journal Article Conference Proceeding
Language:English
Published: New Delhi Springer India 01-03-2018
Springer Nature B.V
Subjects:
Behavioral Sciences
Biomedical and Life Sciences
Galls
Genotypes
Inoculation
Inoculum
Life Sciences
Meloidogyne incognita
Nucleic Acid Chemistry
Plant Biochemistry
Plant breeding
Polymerase chain reaction
Research Article
Solanum lycopersicum
Root-knot nematode
Co-dominant markers
gene
Marker assisted selection
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Summary:In India, tomato ( Solanum lycopersicum L.) suffers yield loss between 11 and 35 % due to root-knot nematode (RKN) ( Meloidogyne  spp.) infestation. Mi 1 gene in tomato confers resistance to the three most damaging RKN species viz, Meloidogyne incognita, M. arenaria and M. javanica. The loss can be averted through the use of cultivars containing Mi 1 gene or incorporating the gene in new cultivars. To identify resistant genotypes, in 2013–2014 a total of 32 genotypes were screened against M. incognita at 2000 J 2 /Kg inoculum. Genotypes, Motelle and H-88-78-1 have showed immune reaction. Mogor and Hisar Lalit also showed resistance reaction. In 2014–2015, nine genotypes including four immune/resistant lines from the last year were screened against RKN at two inoculation rates of 2000 and 4000 J 2 . Reaction of Motelle was similar at both inoculum levels. In other resistant genotypes there was increase in mean of gall index when the inoculum levels were increased. The genetic background had an effect on the variations observed in number of galls produced in the resistant genotypes. Marker assisted selection for resistant genes greatly enhance selection of resistant plants in breeding. To identify suitable marker, six polymerase chain reaction based co-dominant markers were used for molecular screening of Mi 1 gene. Four markers namely, REX-1, CT119, Mi23 and Pmi gave banding pattern in accordance with the reported literature. Considering tight linkage with the gene and assay cost two sequences characterized amplified region markers, Mi23 and Pmi can be preferred over other markers for selecting Mi 1 containing genotypes.
ISSN:0369-8211
2250-1746
DOI:10.1007/s40011-016-0731-1