Anti-adipogenic effects of viscothionin in 3T3-L1 adipocytes and high fat diet induced obesity mice

Viscum album subsp. Coloratum , also known as Korean mistletoe, is a traditional herb that has more recently been used for the treatment of nervine, hypertensive and cardiovascular diseases. Therefore, this study was undertaken to access the anti-obesity effect of Korean mistletoe-derived polypeptid...

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Bibliographic Details
Published in:	Applied biological chemistry Vol. 63; no. 1; pp. 1 - 9
Main Authors:	Kim, Sokho, Ahn, Seo-Hyun, Park, Jong-Heum, Park, Chan Hum, Sin, Yu Su, Shin, Gee-Wook, Kwon, Jungkee
Format:	Journal Article
Language:	English
Published:	Singapore Springer Singapore 01-12-2020 Springer Nature B.V 한국응용생명화학회
Subjects:	Activation Adenosine kinase Adenosine monophosphate Adipocytes AKT protein AMP Applied Microbiology Biological Techniques Bioorganic Chemistry Body weight Cardiovascular diseases Cell differentiation Chemistry Chemistry and Materials Science Diet Differentiation Fatty acids Fatty-acid synthase Glycogen Glycogen synthase kinase 3 Glycogens High fat diet Kinases Lipids Medical treatment Obesity Oils & fats Oral administration Phosphorylation Polypeptides Protein kinase Proteins Simvastatin Sterol regulatory element-binding protein 농학 In vivo mouse model Obesity AMPK Viscothionin
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Summary:	Viscum album subsp. Coloratum , also known as Korean mistletoe, is a traditional herb that has more recently been used for the treatment of nervine, hypertensive and cardiovascular diseases. Therefore, this study was undertaken to access the anti-obesity effect of Korean mistletoe-derived polypeptide viscothionin using 3T3-L1 adipocytes in vitro and in vivo mouse experimental model. Viscothionin (up to 5 μM) was used to treat mouse 3T3-L1 pre-adipocytes during adipocyte differentiation. Adipocyte differentiation in 3T3-L1 cells was confirmed by Oil Red O staining. Obesity was induced by a high-fat diet (HFD) in C57BL/6J mice, followed by oral administration of viscothionin (up to 10 mg/kg) for 3 weeks. As a result, viscothionin (5 μM) inhibited differentiation of adipocyte cells and attenuated accumulation of intracellular lipids through activation of 5′-adenosine monophosphate-activated protein kinase (AMPK), by down-regulating phosphorylation in AKT and glycogen synthase kinase 3β (GSK3β). Treatment of viscothionin also decreased the levels of sterol regulatory element binding protein-1 (SREBP-1) and its target gene, fatty acid synthase (FAS). Moreover, viscothionin (10 mg/kg) significantly suppressed body weight and fat content, and improved serum lipid concentration, compared with the standard drug simvastatin (10 mg/kg), a well-known anti-obesity agent. The present study suggests, that viscothionin exerts anti-adipogenic effect through the activation of AMPK and has potential to prevent HFD-induced obesity.
ISSN:	2468-0834 2468-0842
DOI:	10.1186/s13765-020-0489-2