Search Results - "Sims, P.F.G."

Structural analysis of alpha-helical proteins from wool using cysteine labelling and mass spectrometry by O’Cualain, R.D.M., Sims, P.F.G., Carr, C.M.

“…A simple reduction/labelling/extraction protocol has been developed to fractionate cortical matrix proteins from filament proteins in wool. Through…”
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A reporter system for analysis of regulatable promoter functions in the basidiomycete fungus Phanerochaete chrysosporium by Birch, P.R.J., Sims, P.F.G., Broda, P.

“…To establish a system for functional analysis of regulatable gene promoters involved in lignocellulose degradation by Phanerochaete chrysosporium, a DNA…”
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Characterization of three genes encoding enzymes of the folate biosynthetic pathway in Plasmodium falciparum by Lee, C S, Salcedo, E, Wang, Q, Wang, P, Sims, P F, Hyde, J E

“…Although the folate metabolic pathway in malaria parasites is a major chemotherapeutic target, resistance to currently available antifolate drugs is an…”
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PCR-mediated analysis of lignocellulolytic gene transcription by Phanerochaete chrysosporium: substrate-dependent differential expression within gene families by Broda, P. (University of Manchester Institute of Science and Technology, Manchester, United Kingdom.), Birch, P.R.J, Brooks, P.R, Sims, P.F.G

“…We compare the kinetics of appearance of supernatant enzyme activities (lignin peroxidase, manganese peroxidase, and cellulase) and gene expression (LIC, mnp,…”
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Substrate-dependent differential splicing of introns in the regions encoding the cellulose binding domains of two exocellobiohydrolase I-like genes in Phanerochaete chrysosporium by Birch, P.R.J. (University of Manchester Institute of Science and Technology, Manchester, UK.), Sims, P.F.G, Broda, P

“…Recently, we have shown differential splicing of an intron in the cbhI.2 gene of Phanerochaete chrysosporium ME446; this intron lies within the region of the…”
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Isolation, characterization, and analysis of the expression of the cbhII gene of Phanerochaete chrysosporium by Tempelaars, C.A.M. (University of Manchester, Manchester, UK.), Birch, P.R.J, Sims, P.F.G, Broda, P

“…Two cDNA sequences representing putative allelic variants of the Phanerochaete chrysosporium cbhII gene were isolated by hybridization to the Trichoderma…”
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Definition of the substrate specificity of the ‘sensing’ xylanase of Streptomyces cyaneus using xylooligosaccharide and cellooligosaccharide glycosides of 3,4-dinitrophenol by Zhao, Youngxin, Chany, Calvin J., Sims, Paul F.G., Sinnott, Michael L.

“…The title compounds, (Xyl pβ(1 → 4)) nXyl pβ-3,4-DNP ( n = 0–4) have been made by selective anomeric deprotection of peracetylated xylose oligosaccharides with…”
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Expression of a single lignin peroxidase-encoding gene in Phanerochaete chrysosporium strain ME446 by James, C M, Felipe, M S, Sims, P F, Broda, P

“…A previously described linked set of lignin peroxidase-encoding genes (Lpo) from Phanerochaete chrysosporium (P.c.) ME446 is not expressed under standard…”
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The trpC gene of Phanerochaete chrysosporium is unique in containing an intron but nevertheless maintains the order of functional domains seen in other fungi by Schrank, A, Tempelaars, C, Sims, P F, Oliver, S G, Broda, P

“…The Phanerochaete chryososporium trpC gene has been isolated by complementation of an Escherichia coli trpC mutant. The full extent of the fungal gene,…”
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Nucleotide sequence of the dihydrofolate reductase gene of methotrexate-resistant Lactobacillus casei by Andrews, J, Clore, G M, Davies, R W, Gronenborn, A M, Gronenborn, B, Kalderon, D, Papadopoulos, P C, Schäfer, S, Sims, P F, Stancombe, R

“…The nucleotide sequence of the dihydrofolate reductase (DHFR) gene of a methotrexate-resistant strain of Lactobacillus casei, which is the source of DHFR for…”
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Escherichia coli-yeast shuttle cosmid with positive selection for inserted fragments by Gent, M.E, Crowley, P, Ludwig, J.R, Anwar, R, Sugden, D.A, Sims, P.F.G, Oliver, S.G

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A modified two primer approach to oligonucleotide-directed in vitro mutagenesis by Sims, P F, Minter, S J, Stancombe, R, Gent, M E, Andrews, J, Waring, R B, Towner, P, Davies, R W

“…Using oligonucleotide-directed mutagenesis, we are trying to define the features of the protein structure that are important for the DNA and c-AMP binding by…”
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Nucleotide sequence of a gene from Phanerochaete chrysosporium that shows homology to the facA gene of Aspergillus nidulans by Birch, P R, Sims, P F, Broda, P

“…Heterologous hybridisation was used to isolate a genomic DNA sequence from Phanerochaete chrysosporium using the facA (acetyl CoA synthetase) gene from…”
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