Laser and LED phototherapies on angiogenesis

Angiogenesis is a key process for wound healing. There are few reports of LED phototherapy on angiogenesis, mainly in vivo. The aim of the present investigation was to evaluate histologically the angiogenesis on dorsal cutaneous wounds treated with laser (660 and 790 nm) or LEDs (700, 530, and 460 n...

Full description

Saved in:

Bibliographic Details
Published in:	Lasers in medical science Vol. 28; no. 3; pp. 981 - 987
Main Authors:	de Sousa, Ana Paula Cavalcanti, Paraguassú, Gardênia Matos, Silveira, Nara Tayene Teixeira, de Souza, José, Cangussú, Maria Cristina Teixeira, dos Santos, Jean Nunes, Pinheiro, Antonio Luiz Barbosa
Format:	Journal Article
Language:	English
Published:	London Springer-Verlag 01-05-2013 Springer Nature B.V
Subjects:	Angiogenesis Animals Dentistry Lasers Light therapy Low-Level Light Therapy Male Medicine Medicine & Public Health Neovascularization, Physiologic - radiation effects Optical Devices Optics Original Article Photonics Phototherapy Quantum Optics Rats Rats, Wistar Rodents Skin - blood supply Skin - injuries Skin - radiation effects Wound healing Wound Healing - radiation effects Blood vessels Light therapy Healing
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Angiogenesis is a key process for wound healing. There are few reports of LED phototherapy on angiogenesis, mainly in vivo. The aim of the present investigation was to evaluate histologically the angiogenesis on dorsal cutaneous wounds treated with laser (660 and 790 nm) or LEDs (700, 530, and 460 nm) in a rodent model. Twenty-four young adult male Wistar rats weighting between 200 and 250 g were used on the present study. Under general anesthesia, one excisional wound was created on the dorsum of each animal that were then randomly distributed into six groups with four animals each: G0—control; G1—laser λ 660 nm (60 mW, ϕ ∼2 mm, 10 J/cm 2 ); G2—laser λ 790 nm (50 mW, ϕ ∼2 mm, 10 J/cm 2 ); G3—LED λ 700 ± 20 nm (15 mW, ϕ ∼16 mm, 10 J/cm 2 ); G4—LED λ 530 ± 20 nm (8 mW, ϕ ∼16 mm, 10 J/cm 2 ); G5—LED λ 460 ± 20 nm (22 mW, ϕ ∼16 mm, 10 J/cm 2 ). Irradiation started immediately after surgery and was repeated every other day for 7 days. Animal death occurred at the eighth day after surgery. The specimens were removed, routinely processed to wax, cut and stained with HE. Angiogenesis was scored by blood vessel counting in the wounded area. Quantitative results showed that green LED ( λ 530 ± 20 nm), red LED ( λ 700 ± 20 nm), λ 790 nm laser and λ 660 nm laser caused significant increased angiogenesis when compared to the control group. It is concluded that both laser and LED light are capable of stimulating angiogenesis in vivo on cutaneous wounds and that coherence was not decisive on the outcome of the treatment.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0268-8921 1435-604X
DOI:	10.1007/s10103-012-1187-z