Molecular analysis of G3P[6] rotavirus in the Amazon region of Brazil: evidence of reassortment with equine-like strains

Molecular analysis of G3P[6] rotavirus in the Amazon region of Brazil: evidence of reassortment with equine-like strains

To perform a molecular analysis of rotavirus A (RVA) G3P[6] strains detected in 2012 and 2017 in the Amazon region of Brazil. Eighteen RVA G3P[6] strains were collected from children aged under 10 years hospitalized with acute gastroenteritis, and partial sequencing of each segment genome was perfor...

Full description

Saved in:
Bibliographic Details
Published in:Future microbiology Vol. 16; no. 12; pp. 847 - 862
Main Authors: Silva Serra, Ana C, Júnior, Edivaldo Cs, Cruz, Jonas F, Lobo, Patrícia S, Júnior, Edvaldo Tp, Bandeira, Renato S, Bezerra, Delana Am, Mascarenhas, Joana Dp, Santos Guerra, Sylvia F, Soares, Luana S
Format: Journal Article
Language:English
Published: England Future Medicine Ltd 01-08-2021
Subjects:
Animals
Brazil
Child
diarrhea
evolutionary mechanisms
G3P genotype
Genome, Viral
Horses
Humans
Phylogeny
Reassortant Viruses
reassortment
Rotavirus - genetics
Rotavirus - isolation & purification
Brazil
reassortment
evolutionary mechanisms
diarrhea
G3P genotype
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:To perform a molecular analysis of rotavirus A (RVA) G3P[6] strains detected in 2012 and 2017 in the Amazon region of Brazil. Eighteen RVA G3P[6] strains were collected from children aged under 10 years hospitalized with acute gastroenteritis, and partial sequencing of each segment genome was performed using Sanger sequencing. Phylogenetic analysis showed that all G3P[6] strains had a DS-1-like genotype constellation. Two strains had the highest nucleotide identities with equine-like G3P[6]/G3P[8] genotypes. Several amino acid alterations in VP4 and VP7 neutralizing epitopes of equine-like RVA G3P[6] strains were observed in comparison with vaccine strains. These findings suggest that equine-like RVA G3P[6] strains have been circulating in the Amazon region of Brazil as a result of direct importation, and support natural RVA evolutionary mechanisms.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1746-0913
1746-0921
DOI:10.2217/fmb-2020-0002