Proteome changes in lamb semimembranosus muscles associated with the inclusion of sunflower cake in their diet

Proteome changes in lamb semimembranosus muscles associated with the inclusion of sunflower cake in their diet

Summary A diet based on sunflower cake for lambs was assayed in order to reuse biodiesel industrial by‐products with the aim of reducing livestock costs and evaluating their influence on meat quality. To achieve these goals, sixteen male lambs were fed diets containing different levels of sunflower...

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Bibliographic Details
Published in:International journal of food science & technology Vol. 55; no. 3; pp. 995 - 1001
Main Authors: Melo, Aline Moreira Portela, López‐Pedrouso, María, Costa, Roberto Germano, Franco, Daniel, Alencar Araripe Noronha Moura, Arlindo, Silva, Taciane Alves, Moreno, Frederico Bruno Mendes Batista, Lima Júnior, Valdi, Oliveira Monteiro‐Moreira, Ana Cristina, Medeiros, Ariosvaldo Nunes, Azevedo Moreira, Renato, Lorenzo, Jose M.
Format: Journal Article
Language:English
Published: Oxford Wiley Subscription Services, Inc 01-03-2020
Subjects:
ALDH1A1
Biodiesel fuels
Biofuels
Biomarkers
Cakes
Dehydrogenase
Dehydrogenases
Diet
Dimensional analysis
Electrophoresis
Gels
Helianthus
Livestock
Malate dehydrogenase
Mass spectrometry
Mass spectroscopy
MDH1
Meat
metabolic proteins
Metabolism
Muscles
Phosphopyruvate hydratase
Proteins
Proteomes
Retina
Retinaldehyde dehydrogenase
Structural proteins
Sunflowers
tenderness
two‐dimensional electrophoresis (2‐DE)
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Summary:Summary A diet based on sunflower cake for lambs was assayed in order to reuse biodiesel industrial by‐products with the aim of reducing livestock costs and evaluating their influence on meat quality. To achieve these goals, sixteen male lambs were fed diets containing different levels of sunflower cake (control, 5%, 10% and 15%). Afterwards, their semimembranosus muscles were analysed by two‐dimensional electrophoresis coupled to mass spectrometry and their functional protein association was examined using STRING. Structural and metabolic proteins in the lambs’ proteomes changed significantly according to their diet. Fifteen proteins showed significant changes caused by the inclusion of sunflower cake, and the most differentially abundant structural proteins were detected in 2‐DE gels from the lambs. Differentially abundant metabolic proteins such as ENO3 (enolase 3), MDH1 (malate dehydrogenase) and ALDH1A1 (retinal dehydrogenase) have been proposed as biomarkers of quality parameters in other species. Proteomic analysis revealed that the inclusion of sunflower cake in lamb feeding had a significant impact on the proteome of semimembranosus muscle. Structural proteins of lamb resulted a higher level of proteolysis with the lowest concentrations of sunflower cake in the diet. Metabolic proteins such as malate dehydrogenase (MDH1), enolase (ENO3), glycogen phosphorylase (PYGM), ATP synthase subunit alpha (ATPA) and retinal dehydrogenase (ALDH1A1) significantly changed with diet variations.
ISSN:0950-5423
1365-2621
DOI:10.1111/ijfs.14328