Antibodies against denatured HLA class II molecules detected in luminex-single antigen assay

Abstract False-positive anti-HLA reactions may occur in Luminex-single antigen (SA) beads assays, and it is important to recognize them to correctly interpret the test. The purpose of this report is to describe a peculiar pattern of reactivity, characterized by positivity with beads coated with HLA-...

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Bibliographic Details
Published in:	Human immunology Vol. 74; no. 10; pp. 1300 - 1303
Main Authors:	Grenzi, Patricia C, de Marco, Renato, Silva, Rosemeire Z.R, Campos, Érika F, Gerbase-DeLima, Maria
Format:	Journal Article
Language:	English
Published:	United States Elsevier Inc 01-10-2013
Subjects:	Allergy and Immunology Antibodies - blood Antibodies - immunology Case-Control Studies Female Flow Cytometry Gene Frequency Histocompatibility Antigens Class II - chemistry Histocompatibility Antigens Class II - genetics Histocompatibility Antigens Class II - immunology Histocompatibility Testing Humans Immunoassay - methods Kidney Transplantation Male Protein Denaturation Reagent Kits, Diagnostic
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Summary:	Abstract False-positive anti-HLA reactions may occur in Luminex-single antigen (SA) beads assays, and it is important to recognize them to correctly interpret the test. The purpose of this report is to describe a peculiar pattern of reactivity, characterized by positivity with beads coated with HLA-DRB1∗ 09:01, DRB3∗ 01:01, DRB3∗ 02:02, DRB3∗ 03:01, DPB1∗ 02:01, DPB1∗ 20:01 and DPB1∗ 28:01, that was observed in 141 of 8121 serum samples tested in our laboratory with three different lots of the same kit (LABScreen® SA, One Lambda). These 141 serum samples came from 56 different patients on the kidney transplant waiting list, corresponding to 1% of the patients. Of these, 10 males had never been transfused or transplanted. About 66% of the patients had positive reactions against self-antigen HLA-DRB3 alleles. No reactions against native HLA-DRB1∗ 09:01 were observed in flow cytometry crossmatch and in absorption/elution experiments, leading to the conclusion that the reactivity was due to antibodies against epitopes present in denatured forms of HLA-class II antigens. The occurrence of this reactivity pattern was associated with female gender and systemic lupus erythematosus (SLE).
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0198-8859 1879-1166
DOI:	10.1016/j.humimm.2013.06.035