Gallic acid promotes the in vitro development of sheep secondary isolated follicles involving the phosphatidylinositol 3-kinase pathway

•Gallic acid (100 μM) maintains survival and improves sheep secondary follicle growth.•Gallic acid increases GSH expression and metabolically active mitochondria.•Gallic acid induces meiotic resumption of in vitro grown oocytes.•Gallic acid induces follicle development likely through the PI3K pathwa...

Full description

Saved in:

Bibliographic Details
Published in:	Animal reproduction science Vol. 230; p. 106767
Main Authors:	Silva, Gizele A.L., Araújo, Luana B., Silva, Larissa C.R., Gouveia, Bruna B., Barberino, Ricássio S., Lins, Thae Lanne B.G., Monte, Alane. P.O., Macedo, Taís J.S., Santos, Jamile M.S., Menezes, Vanúzia G., Silva, Regina L.S., Matos, Maria Helena T.
Format:	Journal Article
Language:	English
Published:	Elsevier B.V 01-07-2021
Subjects:	Antioxidant Oocyte Ovine PI3K Preantral follicle Ovine PI3K Antioxidant Oocyte Preantral follicle
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	•Gallic acid (100 μM) maintains survival and improves sheep secondary follicle growth.•Gallic acid increases GSH expression and metabolically active mitochondria.•Gallic acid induces meiotic resumption of in vitro grown oocytes.•Gallic acid induces follicle development likely through the PI3K pathway. This study was conducted to evaluate the effect of addition of gallic acid as the single antioxidant to the base medium for in vitro culture of sheep secondary follicles and if the phosphatidylinositol 3-kinase (PI3K) pathway is involved in the action of gallic acid. Secondary follicles were isolated and cultured for 12 days in α-MEM supplemented with bovine serum albumin (BSA), insulin, glutamine, hypoxanthine, transferrin, selenium, and ascorbic acid (control medium: α-MEM+) or in α-MEM supplemented with BSA, insulin, glutamine, hypoxanthine and different concentrations of gallic acid (25, 50 or 100 μM), thus replacing transferrin, selenium and ascorbic acid in the medium. Follicle morphology, glutathione (GSH), and mitochondrial activity, and meiotic resumption were evaluated. Furthermore, inhibition of PI3K pathway was performed by pretreatment with LY294002. After 12 days of culture, the follicle survival in a medium containing 100 μM gallic acid was similar (P > 0.05) to α-MEM+ and greater (P < 0.05) compared with other gallic acid concentrations. Antrum formation, follicle diameter, GSH, and mitochondrial activity, and meiotic resumption, however, were greater (P < 0.05) when 100 μM gallic acid was included in the α-MEM+ culture medium compared with the control medium. Furthermore, LY294002 inhibited (P < 0.05) follicle survival, development, and meiotic resumption stimulated by 100 μM gallic acid. In conclusion, concentration of 100 μM of gallic acid can be a substitute for transferrin, selenium, and ascorbic acid in the base medium during in vitro culture of sheep secondary follicles, inducing follicle development likely through the PI3K pathway.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0378-4320 1873-2232
DOI:	10.1016/j.anireprosci.2021.106767