Search Results - "Silva, Jose C R"

Stat3 Activation Is Limiting for Reprogramming to Ground State Pluripotency by Yang, Jian, van Oosten, Anouk L., Theunissen, Thorold W., Guo, Ge, Silva, Jose C.R., Smith, Austin

“…The cytokine leukemia inhibitory factor (Lif) sustains self-renewal of mouse embryonic and induced pluripotent stem cells by activating Jak kinase and the…”
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Why it is important to study human–monkey embryonic chimeras in a dish by De Los Angeles, Alejandro, Regenberg, Alan, Mascetti, Victoria, Benvenisty, Nissim, Church, George, Deng, Hongkui, Izpisua Belmonte, Juan Carlos, Ji, Weizhi, Koplin, Julian, Loh, Yuin-Han, Niu, Yuyu, Pei, Duanqing, Pera, Martin, Pho, Nam, Pinzon-Arteaga, Carlos, Saitou, Mitinori, Silva, Jose C. R., Tao, Tan, Trounson, Alan, Warrier, Tushar, Zambidis, Elias T.

“…The study of human–animal chimeras is fraught with technical and ethical challenges. In this Comment, we discuss the importance and future of human–monkey…”
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Self-renewing human naïve pluripotent stem cells dedifferentiate in 3D culture and form blastoids spontaneously by Guo, Mingyue, Wu, Jinyi, Chen, Chuanxin, Wang, Xinggu, Gong, An, Guan, Wei, Karvas, Rowan M., Wang, Kexin, Min, Mingwei, Wang, Yixuan, Theunissen, Thorold W., Gao, Shaorong, Silva, José C. R.

“…Human naïve pluripotent stem cells (hnPSCs) can generate integrated models of blastocysts termed blastoids upon switch to inductive medium. However, the…”
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Zfp281 mediates Nanog autorepression through recruitment of the NuRD complex and inhibits somatic cell reprogramming by Fidalgo, Miguel, Faiola, Francesco, Pereira, Carlos-Filipe, Ding, Junjun, Saunders, Arven, Gingold, Julian, Schaniel, Christoph, Lemischka, Ihor R, Silva, José C. R, Wang, Jianlong

“…The homeodomain transcription factor Nanog plays an important role in embryonic stem cell (ESC) self-renewal and is essential for acquiring ground-state…”
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StemBond hydrogels control the mechanical microenvironment for pluripotent stem cells by Labouesse, Céline, Tan, Bao Xiu, Agley, Chibeza C., Hofer, Moritz, Winkel, Alexander K., Stirparo, Giuliano G., Stuart, Hannah T., Verstreken, Christophe M., Mulas, Carla, Mansfield, William, Bertone, Paul, Franze, Kristian, Silva, José C. R., Chalut, Kevin J.

“…Studies of mechanical signalling are typically performed by comparing cells cultured on soft and stiff hydrogel-based substrates. However, it is challenging to…”
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One-step generation of conditional and reversible gene knockouts by Andersson-Rolf, Amanda, Mustata, Roxana C, Merenda, Alessandra, Kim, Jihoon, Perera, Sajith, Grego, Tiago, Andrews, Katie, Tremble, Katie, Silva, José C R, Fink, Juergen, Skarnes, William C, Koo, Bon-Kyoung

“…The combination of knocking one allele out with CRISPR-mediated NHEJ and targeting the other with a conditionally inactivating cassette allows rapid generation…”
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Histone variant macroH2A marks embryonic differentiation in vivo and acts as an epigenetic barrier to induced pluripotency by Pasque, Vincent, Radzisheuskaya, Aliaksandra, Gillich, Astrid, Halley-Stott, Richard P, Panamarova, Maryna, Zernicka-Goetz, Magdalena, Surani, M Azim, Silva, José C R

“…How cell fate becomes restricted during somatic cell differentiation is a long-lasting question in biology. Epigenetic mechanisms not present in pluripotent…”
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Reprogramming capacity of Nanog is functionally conserved in vertebrates and resides in a unique homeodomain by Theunissen, Thorold W, Costa, Yael, Radzisheuskaya, Aliaksandra, van Oosten, Anouk L, Lavial, Fabrice, Pain, Bertrand, Castro, L Filipe C, Silva, José C R

“…Pluripotency is a developmental ground state that can be recreated by direct reprogramming. Establishment of pluripotency is crucially dependent on the…”
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Switching on pluripotency: a perspective on the biological requirement of Nanog by Theunissen, Thorold W., Silva, José C. R.

“…Pluripotency is a transient cellular state during early development which can be recreated in vitro by direct reprogramming. The molecular mechanisms driving…”
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NANOG-dependent function of TET1 and TET2 in establishment of pluripotency by Costa, Yael, Ding, Junjun, Theunissen, Thorold W., Faiola, Francesco, Hore, Timothy A., Shliaha, Pavel V., Fidalgo, Miguel, Saunders, Arven, Lawrence, Moyra, Dietmann, Sabine, Das, Satyabrata, Levasseur, Dana N., Li, Zhe, Xu, Mingjiang, Reik, Wolf, Silva, José C. R., Wang, Jianlong

“…The authors show that the pluripotency factor NANOG interacts with TET1 and TET2 methylcytosine hydroxylases, and that the hydroxylation of 5-methylcytosine to…”
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OCT4 induces embryonic pluripotency via STAT3 signaling and metabolic mechanisms by Stirparo, Giuliano G., Kurowski, Agata, Yanagida, Ayaka, Bates, Lawrence E., Strawbridge, Stanley E., Hladkou, Siarhei, Stuart, Hannah T., Boroviak, Thorsten E., Silva, Jose C. R., Nichols, Jennifer

“…OCT4 is a fundamental component of the molecular circuitry governing pluripotency in vivo and in vitro. To determine how OCT4 establishes and protects the…”
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Citrullination regulates pluripotency and histone H1 binding to chromatin by Christophorou, Maria A., Castelo-Branco, Gonçalo, Halley-Stott, Richard P., Oliveira, Clara Slade, Loos, Remco, Radzisheuskaya, Aliaksandra, Mowen, Kerri A., Bertone, Paul, Silva, José C. R., Zernicka-Goetz, Magdalena, Nielsen, Michael L., Gurdon, John B., Kouzarides, Tony

“…This study shows that PADI4-mediated citrullination occurs during pluripotency and that citrullination of H1 results in loosening of chromatin compaction;…”
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A defined Oct4 level governs cell state transitions of pluripotency entry and differentiation into all embryonic lineages by Radzisheuskaya, Aliaksandra, Le Bin Chia, Gloryn, dos Santos, Rodrigo L., Theunissen, Thorold W., Castro, L. Filipe C., Nichols, Jennifer, Silva, José C. R.

“…Oct4 is considered a master transcription factor for pluripotent cell self-renewal, but its biology remains poorly understood. Here, we investigated the role…”
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Long‐Term Perfusion Culture of Monoclonal Embryonic Stem Cells in 3D Hydrogel Beads for Continuous Optical Analysis of Differentiation by Kleine‐Brüggeney, Hans, van Vliet, Liisa D., Mulas, Carla, Gielen, Fabrice, Agley, Chibeza C., Silva, José C. R., Smith, Austin, Chalut, Kevin, Hollfelder, Florian

“…Developmental cell biology requires technologies in which the fate of single cells is followed over extended time periods, to monitor and understand the…”
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Reprogramming human cells to naïve pluripotency: how close are we? by Bates, Lawrence E, Silva, José CR

“…•Human naïve-like cells have been generated.•Human naïve-like cells show close similarity to mouse naïve cells and human blastocysts.•Molecular differences to…”
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ZMYM2 inhibits NANOG-mediated reprogramming by Lawrence, Moyra, Theunissen, Thorold W, Lombard, Patrick, Adams, David J, Silva, José C R

“…NANOG is a homeodomain-containing transcription factor which forms one of the hubs in the pluripotency network and plays a key role in the reprogramming of…”
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3D in vitro culture: a peek into primate gastrulation and organogenesis by Li, Huanhuan, Chen, Chuanxin, Silva, José C.R.

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Reprogramming Stars #16: Reprogramming, from Cells to Embryos-An Interview with Dr. José Silva by Silva, José C R, Pereira, Carlos-Filipe

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Do all roads lead to Oct4? The emerging concepts of induced pluripotency by Radzisheuskaya, Aliaksandra, Silva, José C.R

“…Highlights • Oct4 has unique and diverse functions in reprogramming. • Oct4 is essential for lineage specification. • Oct4 regulates multiple contrasting…”
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MBD3/NuRD Facilitates Induction of Pluripotency in a Context-Dependent Manner by dos Santos, Rodrigo L., Tosti, Luca, Radzisheuskaya, Aliaksandra, Caballero, Isabel M., Kaji, Keisuke, Hendrich, Brian, Silva, José C.R.

“…The Nucleosome Remodeling and Deacetylase (NuRD) complex is essential for embryonic development and pluripotent stem cell differentiation. In this study, we…”
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