The DNA barcode reveals cryptic diversity and a new record for the genus Leporinus (Characiformes, Anostomidae) in the hydrographic basins of central northern Brazil

is one of the most speciose genera of the order Characiformes, with 81 valid species distributed throughout much of Central and South America. The considerable diversity of this genus has generated extensive debate on its classification and internal arrangement. In the present study, we investigated...

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Bibliographic Details
Published in:	PeerJ (San Francisco, CA) Vol. 11; p. e15184
Main Authors:	Nascimento, Maria Histelle Sousa, Aragão, Deborah Gaído, Silva, Jordânia Leticia Nascimento, Lima, Renato Correia, Birindelli, José Luis Olivan, Fraga, Elmary Costa, Barros, Maria Claudene
Format:	Journal Article
Language:	English
Published:	United States PeerJ. Ltd 25-05-2023 PeerJ Inc
Subjects:	Analysis Animals Aquaculture, Fisheries and Fish Science Bar codes Biological diversity Brazil Characiformes - genetics Cytochrome oxidase DNA DNA Barcoding, Taxonomic - methods Freshwater Biology Freshwater fish Genetics Leporinus Molecular Biology Molecular identification Neotropical biodiversity Phylogeny Statistics Systematics Zoology Brazil South America Neotropical biodiversity Systematics Freshwater fish Leporinus Molecular identification
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Summary:	is one of the most speciose genera of the order Characiformes, with 81 valid species distributed throughout much of Central and South America. The considerable diversity of this genus has generated extensive debate on its classification and internal arrangement. In the present study, we investigated the species diversity of the genus in central northern Brazil, and conclude that six valid species- , , , , cf. , and -are found in the hydrographic basins of the Brazilian states of Maranhão, Piauí, and Tocantins. We analyzed 182 sequences of the Cytochrome Oxidase subunit I gene, of which, 157 were obtained from specimens collected from the basins of the Itapecuru, Mearim, Turiaçu, Pericumã, Periá, Preguiças, Parnaíba, and Tocantins rivers. The species delimitation analyses, based on the ABGD, ASAP, mPTP, bPTP, and GMYC methods, revealed the presence of four distinct molecular operational taxonomic units (MOTUs), identified as , , , and (from the Parnaíba River). The bPTP method restricted to a single MOTU, and confirmed the occurrence of this species in the rivers of Maranhão for the first time. The separation of cf. into two clades and the subsequent formation of different operational taxonomic units was consistent with polyphyly in this species, which indicates the existence of cryptic diversity. The arrangement of cf. and in two different clades supports the conclusion that the specimens from Maranhão were misidentified, based on their morphological traits, reflecting the taxonomic inconsistencies that exist among morphologically similar species. Overall, then, the species delimitation methods employed in the present study indicated the presence of six MOTUs- , , , cf. , , and . In the case of two other MOTUs identified in the present study, one ( ) is a new record for the state of Maranhão, and we believe that the other represents a population of from the basin of the Parnaíba River.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	2167-8359 2167-8359
DOI:	10.7717/peerj.15184