Clove oil induces anaesthesia and blunts muscle contraction power in three Amazon fish species

Clove oil induces anaesthesia and blunts muscle contraction power in three Amazon fish species

Clove oil is used as an anaesthetic for many species of fish worldwide; however, relatively few studies have assessed its effectiveness on Amazon fish species and no compelling evidence has ever been reported on the relaxant properties of this oil for skeletal muscle of fish. Thus, the objective of...

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Bibliographic Details
Published in:Fish physiology and biochemistry Vol. 44; no. 1; pp. 245 - 256
Main Authors: Fujimoto, Rodrigo Yudi, Pereira, Débora Martins, Silva, Jessica Cristina Souza, de Oliveira, Laís Cássia Araújo, Inoue, Luis Antonio Kioshi Aoki, Hamoy, Moisés, de Mello, Vanessa Jóia, Torres, Marcelo Ferreira, Barbas, Luis André Luz
Format: Journal Article
Language:English
Published: Dordrecht Springer Netherlands 01-02-2018
Springer Nature B.V
Subjects:
Anaesthesia
Anesthesia
Animal Anatomy
Animal Biochemistry
Animal Physiology
Baths
Biomedical and Life Sciences
Cichlidae
Electromyography
Essential oils
Fish
Fish oils
Freshwater & Marine Ecology
Freshwater fishes
Heros severus
Histology
Life Sciences
Mental depression
Morphology
Muscle contraction
Muscle relaxants
Muscles
Oil
Paracheirodon axelrodi
Pterophyllum scalare
Recovery
Skeletal muscle
Species
Zoology
Electromyogram
Essential oil
Sedation
Muscle relaxant
Eugenol
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Summary:Clove oil is used as an anaesthetic for many species of fish worldwide; however, relatively few studies have assessed its effectiveness on Amazon fish species and no compelling evidence has ever been reported on the relaxant properties of this oil for skeletal muscle of fish. Thus, the objective of this study was to evaluate the latencies to deep anaesthesia and recovery, along with the myorelaxant effect of clove oil on three Amazon fish species: cardinal tetra, Paracheirodon axelrodi , banded cichlid, Heros severus and angelfish, Pterophyllum scalare , submitted to short-term anaesthetic baths. Fish were assayed in three groups of 60 fish each and individually anaesthetized in a completely randomized design with six clove oil concentrations using 10 fish/species/concentration. Electromyographic recordings from dorsal muscle were performed during stages of induction and recovery in which nine fish/species/stage were used. Deep anaesthesia was attained for all concentrations tested, and no mortalities were observed throughout the experiments and after a 48-h observation period. Concentration of 90 μL L −1 and above promoted fast deep anaesthesia (< 3 min) and calm recovery in angelfish and cardinal tetra, whereas the concentration of 60 μL L −1 sufficed to quickly anaesthetize banded cichlid. Times to full recovery were not significantly contrasting among species and occurred within appropriate time threshold (< 5 min). Clove oil exerted a conspicuous depression of muscle contraction power, and therefore can be effectively used as a muscle relaxant agent for P. scalare , P. axelrodi , H. severus and potentially, for other fish species.
ISSN:0920-1742
1573-5168
DOI:10.1007/s10695-017-0430-8