Neuroprotective effect of Portulaca oleracea extracts against 6-hydroxydopamine-induced lesion of dopaminergic neurons

The Portulaca oleracea L. (Portulacaceae) is a cosmopolitan species with a wide range of biological activities, including antioxidant and neuroprotective actions. We investigated the effects of P. oleracea extracts in a 6-hydroxydopamine rat model of Parkinson's disease, a debilitating disorder...

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Published in:	Anais da Academia Brasileira de Ciências Vol. 88; no. 3; pp. 1439 - 1450
Main Authors:	Martins, Waleska B, Rodrigues, Sheyla A, Silva, Hatamy K, Dantas, Camila G, Júnior, Waldecy DE Lucca, Filho, Lauro Xavier, Cardoso, Juliana C, Gomes, Margarete Z
Format:	Journal Article
Language:	English
Published:	Brazil Academia Brasileira de Ciências 01-09-2016
Subjects:	Animals Beldroega doença de Parkinson Dopaminergic Neurons - drug effects Dopaminergic Neurons - enzymology Elementos de Resposta Antioxidante Levodopa Male MULTIDISCIPLINARY SCIENCES Neuroprotective Agents - pharmacology Oxidopamine - antagonists & inhibitors Oxidopamine - toxicity Parkinson Disease - drug therapy Pars Compacta - cytology Pars Compacta - enzymology Portulaca - chemistry Rats Rats, Wistar Sympatholytics - antagonists & inhibitors Sympatholytics - toxicity Tyrosine 3-Monooxygenase - analysis Parkinson Disease Elementos de Resposta Antioxidante Purslane doença de Parkinson Antioxidant Response Elements Levodopa Beldroega
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Summary:	The Portulaca oleracea L. (Portulacaceae) is a cosmopolitan species with a wide range of biological activities, including antioxidant and neuroprotective actions. We investigated the effects of P. oleracea extracts in a 6-hydroxydopamine rat model of Parkinson's disease, a debilitating disorder without effective treatments. Chemical profiles of aqueous and ethanolic extracts of whole plant were analyzed by thin layer chromatography and the antioxidant activity was assessed by 2,2-diphenyl-1-picrilhidrazila method. Male Wistar rats received intrastriatal 6-hydroxydopamine and were treated with vehicle or extracts (oral, 200 and 400 mg/kg) daily for two weeks. The behavioral open field test was conducted at days 1 and 15. Immunohistochemical analysis was performed 4 weeks after surgery to quantify tyrosine-hydroxylase cell counts in the substantia nigra pars compacta. Extracts presented antioxidant activity in concentrations above 300 µg/kg. The chromatographic analysis revealed the presence of Levodopa, alkaloids, flavonoids, saponins, tannins, terpenoids and polysaccharides. Both extracts improved motor recovery 15 days after lesion and protected from tyrosine-hydroxylase cell loss after 4 weeks, but these effects were more evident for the aqueous extract. Because the dopamine precursor is present, in addition to antioxidant compounds and neuroprotective effects, P. oleracea can be considered as potential strategy for treating Parkinson's disease.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0001-3765 1678-2690 1678-2690
DOI:	10.1590/0001-3765201620150574