Method for evaluating rhizoctonia resistance in melon germplasm

ABSTRACT The intensive cultivation of melon, mainly in the Northeast of Brazil, has favored the occurrence of root diseases such as rhizoctonia, caused by the fungus Rhizoctonia solani Kuhn. The use of resistant cultivars is one of the most efficient strategic measures for integrated management of d...

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Published in:	Ciência agronômica Vol. 51; no. 4
Main Authors:	Silva, Gefferson Thiago Mota de Almeida, Oliveira, Frederico Inácio Costa de, Carvalho, Alexya Vitoria Felix, André, Thais Paz Pinheiro, Silva, Christiana de Fátima Bruce da, Aragão, Fernando Antonio Souza de
Format:	Journal Article
Language:	English
Published:	Universidade Federal do Ceará 01-01-2020
Subjects:	AGRONOMY Cucumis melo Damping off Genetic resistance Rhizoctonia solani Damping off Rhizoctonia solani Resistência genética Cucumis melo Tombamento Genetic resistance
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Summary:	ABSTRACT The intensive cultivation of melon, mainly in the Northeast of Brazil, has favored the occurrence of root diseases such as rhizoctonia, caused by the fungus Rhizoctonia solani Kuhn. The use of resistant cultivars is one of the most efficient strategic measures for integrated management of diseases in this crop. It is necessary to use an efficient inoculation method to evaluate rhizoctonia in melon germplasm with the aim of identifying sources of resistance. The objective of this study was to develop an efficient method to inoculate the pathogen to proceed in selection of R. solani resistant melon germplasm. Five experiments were conducted under greenhouse conditions to define the adequate container, growth environment, substrate for inoculum production, aggressiveness of the isolates, inoculum density, manner of inoculation, phenological stage of the plant, and time for evaluation after inoculation. The results obtained showed that for selection of R. solani resistant melon germplasm, the use of seedlings with roots cut during transplanting to pots is recommended. The pots should contain sand with organic inoculum obtained from rice grains at the concentration of 150 mg.kg-1 of soil, associated with inoculation through a puncture in the plant stem by an infested toothpick, without the need for a moist chamber. The most aggressive isolate was CMM-1068.
ISSN:	1806-6690 1806-6690
DOI:	10.5935/1806-6690.20200076