Development of microsatellite markers in Guineagrass (Panicum maximum Jacq.) and their transferability to other tropical forage grass species

Development of microsatellite markers in Guineagrass (Panicum maximum Jacq.) and their transferability to other tropical forage grass species

With 1 figure and 1 table ABSTRACT: The Guineagrass (Panicum maximum Jacq.) is one of the most important tropical forage grasses, but genetic knowledge and tools regarding this species are still limited. Therefore, 20 novel polymorphic microsatellite markers were developed, validated, and employed i...

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Bibliographic Details
Published in:Plant breeding Vol. 130; no. 1; pp. 104 - 108
Main Authors: Sousa, A.C.B, Jungmann, L, Campos, T, Sforça, D.A, Boaventura, L.R, Silva, G.M.B, Zucchi, M.I, Jank, L, Souza, A.P
Format: Journal Article
Language:English
Published: Oxford, UK Blackwell Publishing Ltd 01-02-2011
Blackwell
Subjects:
Agronomy. Soil science and plant productions
Biological and medical sciences
Classical and quantitative genetics. Population genetics. Molecular genetics
Data processing
Fundamental and applied biological sciences. Psychology
Gene polymorphism
Generalities. Genetics. Plant material
Genetic diversity
Genetic markers
Genetic relationship
Genetic resources, diversity
Genetics and breeding of economic plants
Germplasm
Grasses
Guineagrass
Microsatellites
Molecular genetics
molecular marker
Panicum maximum
Plant breeding
Plant material
simple sequence repeat
Monocotyledones
Panicum maximum
Tropical crop
Genetic diversity
Guineagrass
Molecular marker
Gramineae
Microsatellite DNA
Angiospermae
Genetic improvement
Spermatophyta
Fodder crop
simple sequence repeat
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Summary:With 1 figure and 1 table ABSTRACT: The Guineagrass (Panicum maximum Jacq.) is one of the most important tropical forage grasses, but genetic knowledge and tools regarding this species are still limited. Therefore, 20 novel polymorphic microsatellite markers were developed, validated, and employed in estimating genetic relationships among 25 P. maximum genotypes selected from a Brazilian germplasm collection. In addition, they were tested for cross-species amplification in four other forage grass species. The number of alleles observed for each locus ranged from 4 to 12 (average 6.7). The values of polymorphism information content (PIC) varied from 0.41 to 0.83 (average 0.61) and the discriminating power (D) ranged from 0.53 to 0.98 (average 0.72). Cross-amplification demonstrated the potential transferability of these microsatellites to four tropical forage grass species. Cluster analysis based on the unweighted pair group method revealed three distinct groups: two clusters consisted of P. maximum genotypes and a third cluster, consisted of the other tropical forage grass species. The data demonstrated that the microsatellites developed herein have potential for germplasm characterization and genetic diversity analysis in P. maximum and other forage grass species.
Bibliography:http://dx.doi.org/10.1111/j.1439-0523.2010.01779.x
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ArticleID:PBR1779
ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:0179-9541
1439-0523
DOI:10.1111/j.1439-0523.2010.01779.x