In vitro evaluation of the antibacterial effect of Brazilian red propolis ethanol extract in the prevention of periodontal disease in dogs

Dental plaque (DP) is found on the surface of teeth and comprises a community of microorganisms that form a structured biofilm. Bacteria present in DP are potential periodontal pathogens when there is an imbalance in the healthy oral environment, and are precursors of periodontal disease (PD). In do...

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Published in:	Comparative immunology, microbiology and infectious diseases Vol. 92; p. 101924
Main Authors:	Figueiredo, Chrislayne Gonçalves Farias, Santos, Mariel Salvador dos, Santos, Amanda Silva, Silva, Elizabete de Santana, Lima, Bruno, Lucca Junior, Waldecy de, Araujo, Yzila Liziane Farias Maia de, Batista, Marcus Vinicius de Aragão
Format:	Journal Article
Language:	English
Published:	England Elsevier Ltd 01-01-2023
Subjects:	Animals Anti-Bacterial Agents - chemistry Anti-Bacterial Agents - pharmacology Bacteria Brazil Dental biofilm Dental Plaque - prevention & control Dental Plaque - veterinary Dog Diseases - drug therapy Dog Diseases - prevention & control Dogs Ethanol - pharmacology Microbial Sensitivity Tests - veterinary Natural product Periodontal Diseases - drug therapy Periodontal Diseases - prevention & control Periodontal Diseases - veterinary Plant Extracts - pharmacology Prevention Propolis - chemistry Propolis - pharmacology Brazil Prevention Natural product Dental biofilm
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Summary:	Dental plaque (DP) is found on the surface of teeth and comprises a community of microorganisms that form a structured biofilm. Bacteria present in DP are potential periodontal pathogens when there is an imbalance in the healthy oral environment, and are precursors of periodontal disease (PD). In dogs, the treatments, such as mechanical removal, are difficult and expensive to apply. Therefore, in order to seek new therapeutic alternatives to control dental plaque in dogs, Brazilian red propolis ethanol extract (RPEE) was tested to evaluate its antibacterial effect on bacteria isolated from DP of dogs without PD. DP was collected from the supragingival dental surfaces of 10 dogs. Bacterial isolates of DP were identified by PCR and sequencing of 16S rDNA gene. The RPEE was obtained using the ultrasound ethanol extraction technique, and the chemical composition was obtained by HPLC-DAD and UV-spectrophotometry. In total, 29 different bacteria belonging to five genera were identified. Formononetin, biochanin A, liquiritigenin and daidzein were the major constituents of the RPEE. The cytotoxic effect showed cell viability after 24 h above 50 % at all concentrations evaluated. The minimum inhibitory concentration was between 37.5 and 150.0 µg/mL for all bacterial isolates. The minimal bactericidal concentration was between 150 and 1200 µg/mL for Gram-positive and 300–1200 µg/mL for Gram-negative bacteria. The results are promising and suggest that RPEE has significant antibacterial potential against the bacteria present in the DP of healthy dogs. Although further studies are still needed, the results suggest RPEE might be safely used in the prevention of periodontal disease. •Red propolis ethanol extract has antibacterial effect on dog dental plaque.•Red propolis ethanol extract showed no cytotoxic effect.•It is proposed a novel therapeutic alternative to control dog bacterial plaque.
ISSN:	0147-9571 1878-1667
DOI:	10.1016/j.cimid.2022.101924