Detection and virulence potential of a phospholipase D-negative Corynebacterium ulcerans from a concurrent diphtheria and infectious mononucleosis case

Diphtheria by Corynebacterium ulcerans is increasingly occurring in children, adolescents and adults. In addition to diphtheria toxin (DT), phospholipase D (PLD) is considered a virulence factor of C. ulcerans . In the present study, a first case of concurrent diphtheria by a PLD-negative C. ulce...

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Bibliographic Details
Published in:	Antonie van Leeuwenhoek Vol. 112; no. 7; pp. 1055 - 1065
Main Authors:	Simpson-Lourêdo, Liliane, Silva, Cecília M. F., Hacker, Elena, Souza, Nadjla F., Santana, Milena M., Antunes, Camila A., Nagao, Prescilla E., Hirata, Raphael, Burkovski, Andreas, Villas Bôas, Maria Helena S., Mattos-Guaraldi, Ana Luíza
Format:	Journal Article
Language:	English
Published:	Cham Springer International Publishing 01-07-2019 Springer Nature B.V
Subjects:	Adolescent Adolescents Adults Animals Anti-Bacterial Agents - pharmacology Biomedical and Life Sciences Caenorhabditis elegans Camp test Children Collagen Collagen (type I) Corynebacterium Corynebacterium - classification Corynebacterium - drug effects Corynebacterium - genetics Corynebacterium - isolation & purification Corynebacterium Infections - microbiology Diphtheria Diphtheria - microbiology Diphtheria toxin Environmental conditions Fibrinogen Fibronectin Humans Infectious mononucleosis Infectious Mononucleosis - microbiology Life Sciences Male Medical Microbiology Microbiology Mononucleosis Nematodes Original Paper Phospholipase Phospholipase D Phospholipase D - analysis Phospholipase D - metabolism Plant Sciences Proteins Soil Science & Conservation Toxins Virulence Virulence factors Virulence Factors - analysis Virulence Factors - metabolism Worms Co-infection Zoonosis Diphtheria Virulence factors Infectious mononucleosis Corynebacterium ulcerans
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Summary:	Diphtheria by Corynebacterium ulcerans is increasingly occurring in children, adolescents and adults. In addition to diphtheria toxin (DT), phospholipase D (PLD) is considered a virulence factor of C. ulcerans . In the present study, a first case of concurrent diphtheria by a PLD-negative C. ulcerans and infectious mononucleosis (IM) was verified. Clinical and microbiological profiles and binding properties to human Fibrinogen (Fbg), Fibronectin (Fn) and type I collagen (col I) biotinylated proteins and virulence to Caenorhabditis elegans were investigated for C. ulcerans strain 2590 (clinical isolate) and two control strains, including PLD-positive BR-AD22 wild type and PLD-negative ELHA-1 PLD mutant strains. MALDI-TOF assays and a multiplex PCR of genes coding for potentially toxigenic corynebacteria identified strain 2590 as non-DT producing. Interestingly, strain 2590 did not express PLD activity in the CAMP test although the presence of the pld gene was verified. PLD-negative 2590 and a PLD-positive 210932 strains showed similar affinity to Fbg, Fn and type I collagen. C. elegans were able to escape from C. ulcerans strains, independent of PLD and DT production. Higher mortality of nematodes was verified for PLD-negative strains. Additional studies concerning multifactorial virulence potential of C. ulcerans , including environmental conditions remain necessary.
ISSN:	0003-6072 1572-9699
DOI:	10.1007/s10482-019-01240-4