Epigenetic Variability of Ribosomal Cistrons of Acrocentric Chromatids of Chromosomes 14 in Fetuses and Newborns with Down Syndrome

Epigenetic Variability of Ribosomal Cistrons of Acrocentric Chromatids of Chromosomes 14 in Fetuses and Newborns with Down Syndrome

Following the completion of the Human Genome Project, the strategic focus of modern genetics shifted towards functional genomics, encompassing non-coding regions of DNA, including total non-coding DNA and satellite stalks with ribosomal cistrons. These consist of repetitive sequences of nucleotides...

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Bibliographic Details
Published in:Cytology and genetics Vol. 58; no. 5; pp. 513 - 522
Main Authors: Teimuraz Lezhava, Buadze, Tamar, Sigua, Nino, Jokhadze, Tinatin, Gaiozishvili, Maia, Sigua, Tamar, Kulijanashvili, Natia
Format: Journal Article
Language:English
Published: Moscow Pleiades Publishing 01-10-2024
Springer Nature B.V
Subjects:
Amniotic fluid
Biomedical and Life Sciences
Biomedicine
Chromatids
Chromosomes
Cistrons
Diagnosis
Down syndrome
Down's syndrome
Epigenetics
Fetuses
Genetic variability
Heterochromatin
Human Genetics
Human Genome Project
Lymphocytes
Mosaicism
Neonates
Nucleotide sequence
Satellite DNA
Statistical analysis
Trisomy
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Summary:Following the completion of the Human Genome Project, the strategic focus of modern genetics shifted towards functional genomics, encompassing non-coding regions of DNA, including total non-coding DNA and satellite stalks with ribosomal cistrons. These consist of repetitive sequences of nucleotides localized in heterochromatin, the functions of which still require clarification. The present study investigates the activity of ribosomal cistrons within chromatid satellite stalks (NOR heterochromatin) of acrocentric chromosomes in patients with Down Syndrome +21 (DS). The study comprised: 29 healthy fetuses (441 metaphases from cultivated amniotic fluid); 8 fetuses with DS (190 metaphases from cultivated amniotic fluid); 10 healthy newborns (290 metaphases from cultivated lymphocytes); 10 newborn patients with DS (273 metaphases from cultivated lymphocytes) including: 5 newborn patients with trisomy DS (81 metaphases) and 5 mosaic form of newborns with DS (46, XX or 46, XY/47, XX, +21 or 47, XY, +21) (192 metaphases). The activity of ribosomal cistrons of chromatid on the 14 chromosomes included in associations in the control group (healthy fetuses and healthy newborns) corresponding to the order: 21 > 14 = 22 = 13 > 15 and 21 > 14 > 22 > 13 > 15, respectively. In fetuses with DS (21 > 22 > 15 > 13 > 14), in newborns with DS (21 > 13 > 15 = 22 > 14), and with mosaicism of cells of newborns with DS (21 > 13 > 15 > 22 > 14), a statistically significant decrease in the activity of entering associations of chromatids 14 chromosomes was noted. It is noteworthy that, in fetuses, in newborns, and with mosaicism of cells of newborns with DS activity of entering in associations of 14 chromatids with homologous 14 chromatids were not determined (21:21 > 13:13 > 15:15 > 22:22 > 14:14; 21:21 > 22:22 > 13:13 > 15: 15 > 14:14 and 21:21 > 13:13 > 22:22 > 15:15 > 14:14). In fetuses and newborns (control group), the order was: 21:21 > 22:22 > 14:14 > 13:13 = 15:15 and 14 : 14 = 22:22 > 21:21 > 13:13 > 15 : 15, respectively. These results indicate that in DS, the ribosomal cistrons of chromatids on chromosomes 14 undergo epigenetic changes, leading to a reduction in the activity of ribosomal cistrons on these chromatids. This provides a crucial foundation for the prevention, diagnosis, and treatment of DS. The study of ribosomal gene activity at the level of different acrocentric chromosomes in pathologies represents a new direction for medicine, offering insights into both disease diagnosis and the development of novel treatment strategies in the future.
ISSN:0095-4527
1934-9440
DOI:10.3103/S0095452724050074