Search Results - "Sigala, Paul A"

The interdependence of isoprenoid synthesis and apicoplast biogenesis in malaria parasites by Okada, Megan, Sigala, Paul A

“…Isoprenoid precursor synthesis is an ancient and fundamental function of plastid organelles and a critical metabolic activity of the apicoplast in Plasmodium…”
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The peculiarities and paradoxes of Plasmodium heme metabolism by Sigala, Paul A, Goldberg, Daniel E

“…For over a century, heme metabolism has been recognized to play a central role during intraerythrocytic infection by Plasmodium parasites, the causative agent…”
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Doxycycline has distinct apicoplast-specific mechanisms of antimalarial activity by Okada, Megan, Guo, Ping, Nalder, Shai-Anne, Sigala, Paul A

“…Doxycycline (DOX) is a key antimalarial drug thought to kill parasites by blocking protein translation in the essential apicoplast organelle. Clinical use is…”
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Plasmodium heme biosynthesis: To be or not to be essential? by Goldberg, Daniel E, Sigala, Paul A

“…About the Authors: Daniel E. Goldberg * E-mail: dgoldberg@wustl.edu (DEG); p.sigala@utah.edu (PAS) Affiliation: Departments of Medicine and Molecular…”
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Direct tests of cytochrome c and c1 functions in the electron transport chain of malaria parasites by Espino-Sanchez, Tanya J, Wienkers, Henry, Marvin, Rebecca G, Nalder, Shai-anne, García-Guerrero, Aldo E, Vannatta, Peter E, Jami-Alahmadi, Yasaman, Blackwell, Amanda Mixon, Whitby, Frank G, Wohlschlegel, James A, Kieber-Emmons, Matthew T, Hill, Christopher P, Sigala, Paul A

“…The mitochondrial electron transport chain (ETC) of Plasmodium malaria parasites is a major antimalarial drug target, but critical cytochrome (cyt) functions…”
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Determination of Hydrogen Bond Structure in Water versus Aprotic Environments To Test the Relationship Between Length and Stability by Sigala, Paul A, Ruben, Eliza A, Liu, Corey W, Piccoli, Paula M. B, Hohenstein, Edward G, Martínez, Todd J, Schultz, Arthur J, Herschlag, Daniel

“…Hydrogen bonds profoundly influence the architecture and activity of biological macromolecules. Deep appreciation of hydrogen bond contributions to…”
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Critical role for isoprenoids in apicoplast biogenesis by malaria parasites by Okada, Megan, Rajaram, Krithika, Swift, Russell P, Mixon, Amanda, Maschek, John Alan, Prigge, Sean T, Sigala, Paul A

“…Isopentenyl pyrophosphate (IPP) is an essential metabolic output of the apicoplast organelle in malaria parasites and is required for prenylation-dependent…”
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Quantitative, directional measurement of electric field heterogeneity in the active site of ketosteroid isomerase by Fafarman, Aaron T, Sigala, Paul A, Schwans, Jason P, Fenn, Timothy D, Herschlag, Daniel, Boxer, Steven G

“…Understanding the electrostatic forces and features within highly heterogeneous, anisotropic, and chemically complex enzyme active sites and their connection…”
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Direct Tests of Enzymatic Heme Degradation by the Malaria Parasite Plasmodium falciparum by Sigala, Paul A., Crowley, Jan R., Hsieh, Samantha, Henderson, Jeffrey P., Goldberg, Daniel E.

“…Malaria parasites generate vast quantities of heme during blood stage infection via hemoglobin digestion and limited de novo biosynthesis, but it remains…”
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Hydrogen bond dynamics in the active site of photoactive yellow protein by Sigala, Paul A, Tsuchida, Mark A, Herschlag, Daniel

“…Hydrogen bonds play major roles in biological structure and function. Nonetheless, hydrogen-bonded protons are not typically observed by X-ray crystallography,…”
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Divergent acyl carrier protein decouples mitochondrial Fe-S cluster biogenesis from fatty acid synthesis in malaria parasites by Falekun, Seyi, Sepulveda, Jaime, Jami-Alahmadi, Yasaman, Park, Hahnbeom, Wohlschlegel, James A, Sigala, Paul A

“…Most eukaryotic cells retain a mitochondrial fatty acid synthesis (FASII) pathway whose acyl carrier protein (mACP) and 4-phosphopantetheine (Ppant) prosthetic…”
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Deconvoluting heme biosynthesis to target blood-stage malaria parasites by Sigala, Paul A, Crowley, Jan R, Henderson, Jeffrey P, Goldberg, Daniel E

“…Heme metabolism is central to blood-stage infection by the malaria parasite Plasmodium falciparum. Parasites retain a heme biosynthesis pathway but do not…”
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Multiple-Streams Focusing-Based Cell Separation in High Viscoelasticity Flow by Feng, Haidong, Patel, Dhruv, Magda, Jules J., Geher, Sage, Sigala, Paul A., Gale, Bruce K.

“…Viscoelastic flow has been widely used in microfluidic particle separation processes, in which particles get focused on the channel center in diluted…”
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Dissecting the paradoxical effects of hydrogen bond mutations in the ketosteroid isomerase oxyanion hole by Kraut, Daniel A, Sigala, Paul A, Fenn, Timothy D, Herschlag, Daniel

“…The catalytic importance of enzyme active-site interactions is frequently assessed by mutating specific residues and measuring the resulting rate reductions…”
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Testing Geometrical Discrimination within an Enzyme Active Site: Constrained Hydrogen Bonding in the Ketosteroid Isomerase Oxyanion Hole by Sigala, Paul A, Kraut, Daniel A, Caaveiro, Jose M. M, Pybus, Brandon, Ruben, Eliza A, Ringe, Dagmar, Petsko, Gregory A, Herschlag, Daniel

“…Enzymes are classically proposed to accelerate reactions by binding substrates within active-site environments that are structurally preorganized to optimize…”
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Quantitative dissection of hydrogen bond-mediated proton transfer in the ketosteroid isomerase active site by Sigala, Paul A, Fafarman, Aaron T, Schwans, Jason P, Fried, Stephen D, Fenn, Timothy D, Caaveiro, Jose M M, Pybus, Brandon, Ringe, Dagmar, Petsko, Gregory A, Boxer, Steven G, Herschlag, Daniel

“…Hydrogen bond networks are key elements of protein structure and function but have been challenging to study within the complex protein environment. We have…”
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Do Ligand Binding and Solvent Exclusion Alter the Electrostatic Character within the Oxyanion Hole of an Enzymatic Active Site? by Sigala, Paul A, Fafarman, Aaron T, Bogard, Patrick E, Boxer, Steven G, Herschlag, Daniel

“…We report the site-specific incorporation of a thiocyanate vibrational probe into the active-site oxyanion hole of ketosteroid isomerase (KSI) to test the…”
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In-Cell Enzymology To Probe His–Heme Ligation in Heme Oxygenase Catalysis by Sigala, Paul A, Morante, Koldo, Tsumoto, Kouhei, Caaveiro, Jose M. M, Goldberg, Daniel E

“…Heme oxygenase (HO) is a ubiquitous enzyme with key roles in inflammation, cell signaling, heme disposal, and iron acquisition. HO catalyzes the oxidative…”
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Testing electrostatic complementarity in enzyme catalysis: hydrogen bonding in the ketosteroid isomerase oxyanion hole by Kraut, Daniel A, Sigala, Paul A, Pybus, Brandon, Liu, Corey W, Ringe, Dagmar, Petsko, Gregory A, Herschlag, Daniel

“…A longstanding proposal in enzymology is that enzymes are electrostatically and geometrically complementary to the transition states of the reactions they…”
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Identification of a divalent metal transporter required for cellular iron metabolism in malaria parasites by Loveridge, Kade M, Sigala, Paul A

“…malaria parasites invade and multiply inside red blood cells (RBCs), the most iron-rich compartment in humans. Like all cells, requires nutritional iron to…”
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