Search Results - "Siehl, Daniel L"

Discovery and directed evolution of a glyphosate tolerance gene by Castle, L.A, Siehl, D.L, Gorton, R, Patten, P.A, Chen, Y.H, Bertain, S, Cho, H.J, Duck, N, Wong, J, Liu, D

“…The herbicide glyphosate is effectively detoxified by N-acetylation. We screened a collection of microbial isolates and discovered enzymes exhibiting…”
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The Molecular Basis of Glyphosate Resistance by an Optimized Microbial Acetyltransferase by Siehl, Daniel L., Castle, Linda A., Gorton, Rebecca, Keenan, Robert J.

“…GAT is an N-acetyltransferase from Bacillus licheniformis that was optimized by gene shuffling for acetylation of the broad spectrum herbicide, glyphosate,…”
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Non-plastidic, tyrosine-insensitive prephenate dehydrogenases from legumes by Schenck, Craig A, Chen, Siyu, Siehl, Daniel L, Maeda, Hiroshi A

“…Biochemical, bioinformatic and genetic evidence uncover a tyrosine biosynthesis pathway in plants that—in contrast to known plant pathways—occurs in the…”
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Members of the GH3 Family of Proteins Conjugate 2,4-D and Dicamba with Aspartate and Glutamate by Chiu, Li-Wei, Heckert, Matthew J, You, You, Albanese, Nicholas, Fenwick, Tamara, Siehl, Daniel L, Castle, Linda A, Tao, Yumin

“…Auxin homeostasis is a highly regulated process that must be maintained to allow auxin to exert critical growth and developmental controls. Auxin conjugase and…”
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Characterization of a Glyphosate-Tolerant Enzyme from Streptomyces svecius: A Distinct Class of 5‑Enolpyruvylshikimate-3-phosphate Synthases by Griffin, Samantha L, Chekan, Jonathan R, Lira, Justin M, Robinson, Andrew E, Yerkes, Carla N, Siehl, Daniel L, Wright, Terry R, Nair, Satish K, Cicchillo, Robert M

“…Natural and modified versions of the 5-enolpyruvylshikimate-3-phosphate synthase (epsps) gene have been used to confer tolerance to the broad-spectrum…”
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History and Outlook for Glyphosate-Resistant Crops by Green, Jerry M, Siehl, Daniel L

“…Glyphosate-resistant (GR) crops, commercially referred to as glyphosate-tolerant (GT), started the revolution in crop biotechnology in 1996. Growers rapidly…”
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DNA Shuffling as a Tool for Protein Crystallization by Keenan, Robert J., Siehl, Daniel L., Gorton, Rebecca, Castle, Linda A., Stroud, Robert M.

“…The success of structural studies performed on an individual target in small scale or on many targets in the systemwide scale of structural genomics depends…”
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Broad 4-Hydroxyphenylpyruvate Dioxygenase Inhibitor Herbicide Tolerance in Soybean with an Optimized Enzyme and Expression Cassette by Siehl, Daniel L., Tao, Yumin, Albert, Henrik, Dong, Yuxia, Heckert, Matthew, Madrigal, Alfredo, Lincoln-Cabatu, Brishette, Lu, Jian, Fenwick, Tamara, Bermudez, Ericka, Sandoval, Marian, Horn, Caroline, Green, Jerry M., Hale, Theresa, Pagano, Peggy, Clark, Jenna, Udranszky, Ingrid A., Rizzo, Nancy, Bourett, Timothy, Howard, Richard J., Johnson, David H., Vogt, Mark, Akinsola, Goke, Castle, Linda A.

“…With an optimized expression cassette consisting of the soybean (Glycine max) native promoter modified for enhanced expression driving a chimeric gene coding…”
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Novel form of the Michaelis-Menten equation that enables accurate estimation of (kcat/KM)KI with just two rate measurements; utility in directed evolution by Lu, Jian, Dong, Yuxia, Ng, Emily C., Siehl, Daniel L.

“…Abstract One of applications of directed evolution is to desensitize an enzyme to an inhibitor. kcat,1/KM and KI are three dimensions that when multiplied…”
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Novel form of the Michaelis-Menten equation that enables accurate estimation of (kcat/KM)KI with just two rate measurements; utility in directed evolution by Lu, Jian, Dong, Yuxia, Ng, Emily C, Siehl, Daniel L

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An Enterotoxin-Like Binary Protein from Pseudomonas protegens with Potent Nematicidal Activity by Wei, Jun-Zhi, Siehl, Daniel L, Hou, Zhenglin, Rosen, Barbara, Oral, Jarred, Taylor, Christopher G, Wu, Gusui

“…Soil microbes are a major food source for free-living soil nematodes. It is known that certain soil bacteria have evolved systems to combat predation. We…”
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Evolution of a microbial acetyltransferase for modification of glyphosate: a novel tolerance strategy by Siehl, D.L, Castle, L.A, Gorton, R, Chen, Y.H, Bertain, S, Cho, H.J, Keenan, R, Liu, D, Lassner, M.W

“…N-Acetylation is a modification of glyphosate that could potentially be used in transgenic crops, given a suitable acetyltransferase. Weak enzymatic activity…”
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A Naturally Occurring Point Mutation Confers Broad Range Tolerance to Herbicides That Target Acetolactate Synthase by Bernasconi, Paul, Woodworth, Alison R., Rosen, Barbara A., Subramanian, Mani V., Siehl, Daniel L.

“…Acetolactate synthase (ALS) inhibitors are among the most commonly used herbicides. They fall into four distinct families of compounds: sulfonylureas,…”
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Kinetic and regulatory properties of arogenate dehydratase in seedlings of Sorghum bicolor (L.) Moench by Siehl, D L, Conn, E E

“…Arogenate dehydratase was purified sixfold from an extract of etiolated seedlings of Sorghum bicolor. Prephenate dehydratase was not detected. The arogenate…”
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Design of enzyme-targeted agrochemicals by Haworth, Philip, Siehl, Daniel L

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Tissue distribution and subcellular localization of prephenate aminotransferase in leaves of Sorghum bicolor by Siehl, D.L, Singh, B.K, Conn, E.E

“…The tissue and subcellular distribution of prephenate aminotransferase, an enzyme of the shikimate pathway, was investigated in protoplasts from leaves of…”
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Evolution of a microbial acetyltransferase for modification of glyphosate: a novel tolerance strategy by Siehl, Daniel L, Castle, Linda A, Gorton, Rebecca, Chen, Yong Hong, Bertain, Sean, Cho, Hyeon-Je, Keenan, Robert, Liu, Donglong, Lassner, Michael W

“…N-Acetylation is a modification of glyphosate that could potentially be used in transgenic crops, given a suitable acetyltransferase. Weak enzymatic activity…”
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Adenylosuccinate synthetase: site of action of hydantocidin, a microbial phytotoxin by Siehl, D.L. (Sandoz Agro, Inc., Palo Alto, CA.), Subramanian, M.V, Walters, E.W, Lee, S.F, Anderson, R.J, Toschi, A.G

“…The site of action of hydantocidin was probed using Arabidopsis thaliana plants growing on agar plates. Herbicidal effects were reversed when the agar medium…”
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Refined Crystal Structure of Adenylosuccinate Synthetase from Escherichia coli Complexed with Hydantocidin 5‘-Phosphate, GDP, HPO42-, Mg2+, and Hadacidin by POLAND, Bradley W., LEE, Shy-Fuh, SUBRAMANIAN, Mani V., SIEHL, Daniel L., ANDERSON, Richard J., FROMM, Herbert J., HONZATKO, Richard B.

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Adenylosuccinate synthetase from maize. Purification, properties, and mechanism of inhibition by 5'-phosphohydantocidin by Walters, E.W. (Novartis Crop Protection, Palo Alto, CA.), Lee, S.F, Niderman, T, Bernasconi, P, Subramanian, M.V, Siehl, D.L

“…Adenylosuccinate synthetase (AdSS) is the site of action of hydantocidin, a potent microbial phytotoxin. A kinetic analysis of the mode of inhibition of a…”
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