Search Results - "Siegfried, Blair"

Acaricide, fungicide and drug interactions in honey bees (Apis mellifera) by Johnson, Reed M, Dahlgren, Lizette, Siegfried, Blair D, Ellis, Marion D

“…Chemical analysis shows that honey bees (Apis mellifera) and hive products contain many pesticides derived from various sources. The most abundant pesticides…”
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A Comprehensive Selection of Reference Genes for RT-qPCR Analysis in a Predatory Lady Beetle, Hippodamia convergens (Coleoptera: Coccinellidae) by Pan, Huipeng, Yang, Xiaowei, Siegfried, Blair D, Zhou, Xuguo

“…Reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) is a reliable, rapid, and reproducible technique for measuring and evaluating changes in…”
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Responses of two ladybird beetle species (Coleoptera: Coccinellidae) to dietary RNAi by Haller, Simone, Widmer, Franco, Siegfried, Blair D, Zhuo, Xuguo, Romeis, Jörg

“…BACKGROUND One concern with the adoption of RNAi‐based genetically engineered (GE) crops is the potential harm to valued non‐target organisms. Species of…”
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Dietary RNAi toxicity assay exhibits differential responses to ingested dsRNAs among lady beetles by Pan, Huipeng, Yang, Xiaowei, Romeis, Jörg, Siegfried, Blair D, Zhou, Xuguo

“…Background Most recently, major federal regulatory agencies deregulated an in planta RNA interference (RNAi) trait against a devastating corn pest, the western…”
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Evaluation of five methods for total DNA extraction from western corn rootworm beetles by Chen, Hong, Rangasamy, Murugesan, Tan, Sek Yee, Wang, Haichuan, Siegfried, Blair D

“…DNA extraction is a routine step in many insect molecular studies. A variety of methods have been used to isolate DNA molecules from insects, and many…”
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Selection of Reference Genes for RT-qPCR Analysis in the Monarch Butterfly, Danaus plexippus (L.), a Migrating Bio-Indicator by Pan, Huipeng, Yang, Xiaowei, Bidne, Keith, Hellmich, Richard L, Siegfried, Blair D, Zhou, Xuguo

“…Reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) is a powerful technique to quantify gene expression. To facilitate gene expression study…”
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Evidence of Field-Evolved Resistance to Bifenthrin in Western Corn Rootworm (Diabrotica virgifera virgifera LeConte) Populations in Western Nebraska and Kansas by Pereira, Adriano E, Wang, Haichuan, Zukoff, Sarah N, Meinke, Lance J, French, B Wade, Siegfried, Blair D

“…Pyrethroid insecticides have been used to control larvae or adults of the western corn rootworm (WCR), Diabrotica virgifera virgifera LeConte, a key pest of…”
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Selection of reference genes for normalization of RT-qPCR data in gene expression studies in Anthonomus eugenii Cano (Coleoptera: Curculionidae) by Pinheiro, Daniele H., Siegfried, Blair D.

“…The pepper weevil, Anthonomus eugenii Cano (Coleoptera: Curculionidae), is the main insect pest of peppers ( Capsicum spp.) throughout the southern U.S. and a…”
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Towards the elements of successful insect RNAi by Scott, Jeffrey G., Michel, Kristin, Bartholomay, Lyric C., Siegfried, Blair D., Hunter, Wayne B., Smagghe, Guy, Zhu, Kun Yan, Douglas, Angela E.

“…•RNAi is a highly valuable tool for understanding gene function.•RNAi holds great potential for pest management.•RNAi efficiency varies among insect species…”
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Validation of RNA interference in western corn rootworm Diabrotica virgifera virgifera LeConte (Coleoptera: Chrysomelidae) adults by Rangasamy, Murugesan, Siegfried, Blair D

“…BACKGROUND: RNA interference (RNAi) is commonly used in insect functional genomics studies and usually involves direct injection of double‐stranded RNA…”
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Clathrin-dependent endocytosis is associated with RNAi response in the western corn rootworm, Diabrotica virgifera virgifera LeConte by Pinheiro, Daniele H, Vélez, Ana M, Fishilevich, Elane, Wang, Haichuan, Carneiro, Newton P, Valencia-Jiménez, Arnubio, Valicente, Fernando H, Narva, Kenneth E, Siegfried, Blair D

“…The cellular uptake of dsRNA after dietary exposure is critical for RNAi efficiency; however, the mechanism of its uptake in many insects remains to be…”
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CRISPR/Cas9 mediated knockout of the abdominal-A homeotic gene in fall armyworm moth (Spodoptera frugiperda) by Wu, Ke, Shirk, Paul D, Taylor, Caitlin E, Furlong, Richard B, Shirk, Bryce D, Pinheiro, Daniele H, Siegfried, Blair D

“…The fall armyworm (FAW), Spodoptera frugiperda (J.E. Smith), is an important pest of maize in the Americas and has recently been introduced into Africa. Fall…”
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Knockdown of RNA Interference Pathway Genes in Western Corn Rootworms (Diabrotica virgifera virgifera Le Conte) Demonstrates a Possible Mechanism of Resistance to Lethal dsRNA by Vélez, Ana María, Khajuria, Chitvan, Wang, Haichuan, Narva, Kenneth E, Siegfried, Blair D

“…RNA interference (RNAi) is being developed as a potential tool for insect pest management. Increased understanding of the RNAi pathway in target insect pests…”
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Influence of Pyrethroid Resistance on Vector Competency for Zika Virus by Aedes aegypti (Diptera: Culicidae) by Parker-Crockett, Casey, Connelly, C. Roxanne, Siegfried, Blair, Alto, Barry

“…The vector competence of mosquitoes for pathogens has been shown to be influenced by the status of insecticide resistance in the mosquito population. However,…”
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Cross-resistance and synergism bioassays suggest multiple mechanisms of pyrethroid resistance in western corn rootworm populations by E Pereira, Adriano, Souza, Dariane, Zukoff, Sarah N, Meinke, Lance J, Siegfried, Blair D

“…Recently, resistance to the pyrethroid bifenthrin was detected and confirmed in field populations of western corn rootworm, Diabrotica virgifera virgifera…”
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The Use of Insecticides to Manage the Western Corn Rootworm, Diabrotica virgifera virgifera , LeConte: History, Field-Evolved Resistance, and Associated Mechanisms by Meinke, Lance J, Souza, Dariane, Siegfried, Blair D

“…The western corn rootworm, LeConte (Dvv) is a significant insect pest of maize in the United States (U.S.). This paper reviews the history of insecticide use…”
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Delivery of gene‐specific dsRNA by microinjection and feeding induces RNAi response in Sri Lanka weevil, Myllocerus undecimpustulatus undatus Marshall by Pinheiro, Daniele H, Taylor, Caitlin E, Wu, Ke, Siegfried, Blair D

“…BACKGROUND RNA interference (RNAi) is widely used in entomological research for functional analysis of genes and is being considered as a new tool for insect…”
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Validation of reference housekeeping genes for gene expression studies in western corn rootworm (Diabrotica virgifera virgifera) by Rodrigues, Thaís Barros, Barros Rodrigues, Thaís, Khajuria, Chitvan, Wang, Haichuan, Matz, Natalie, Cunha Cardoso, Danielle, Valicente, Fernando Hercos, Zhou, Xuguo, Siegfried, Blair

“…Quantitative Real-time PCR (qRT-PCR) is a powerful technique to investigate comparative gene expression. In general, normalization of results using a highly…”
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Linkage of an ABCC transporter to a single QTL that controls Ostrinia nubilalis larval resistance to the Bacillus thuringiensis Cry1Fa toxin by Coates, Brad S., Siegfried, Blair D.

“…Field evolved resistance of insect populations to Bacillus thuringiensis (Bt) crystalline (Cry) toxins expressed by crop plants has resulted in reduced control…”
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Molecular evolution of glycoside hydrolase genes in the Western corn rootworm (Diabrotica virgifera virgifera) by Eyun, Seong-il, Wang, Haichuan, Pauchet, Yannick, Ffrench-Constant, Richard H, Benson, Andrew K, Valencia-Jiménez, Arnubio, Moriyama, Etsuko N, Siegfried, Blair D

“…Cellulose is an important nutritional resource for a number of insect herbivores. Digestion of cellulose and other polysaccharides in plant-based diets…”
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