Search Results - "Siebert, C."

An introduction to sample preparation and imaging by cryo-electron microscopy for structural biology by Thompson, Rebecca F., Walker, Matt, Siebert, C. Alistair, Muench, Stephen P., Ranson, Neil A.

“…Transmission electron microscopy (EM) is a versatile technique that can be used to image biological specimens ranging from intact eukaryotic cells to…”
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Structural Basis of Vesicle Formation at the Inner Nuclear Membrane by Hagen, Christoph, Dent, Kyle C., Zeev-Ben-Mordehai, Tzviya, Grange, Michael, Bosse, Jens B., Whittle, Cathy, Klupp, Barbara G., Siebert, C. Alistair, Vasishtan, Daven, Bäuerlein, Felix J.B., Cheleski, Juliana, Werner, Stephan, Guttmann, Peter, Rehbein, Stefan, Henzler, Katja, Demmerle, Justin, Adler, Barbara, Koszinowski, Ulrich, Schermelleh, Lothar, Schneider, Gerd, Enquist, Lynn W., Plitzko, Jürgen M., Mettenleiter, Thomas C., Grünewald, Kay

“…Vesicular nucleo-cytoplasmic transport is becoming recognized as a general cellular mechanism for translocation of large cargoes across the nuclear envelope…”
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Acidic pH-Induced Conformations and LAMP1 Binding of the Lassa Virus Glycoprotein Spike by Li, Sai, Sun, Zhaoyang, Pryce, Rhys, Parsy, Marie-Laure, Fehling, Sarah K, Schlie, Katrin, Siebert, C Alistair, Garten, Wolfgang, Bowden, Thomas A, Strecker, Thomas, Huiskonen, Juha T

“…Lassa virus is an enveloped, bi-segmented RNA virus and the most prevalent and fatal of all Old World arenaviruses. Virus entry into the host cell is mediated…”
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Cryo-EM structure of the Blastochloris viridis LH1–RC complex at 2.9 Å by Qian, Pu, Siebert, C. Alistair, Wang, Peiyi, Canniffe, Daniel P., Hunter, C. Neil

“…The light-harvesting 1–reaction centre (LH1–RC) complex is a key functional component of bacterial photosynthesis. Here we present a 2.9 Å resolution…”
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An assessment of the use of Hepatitis B Virus core protein virus-like particles to display heterologous antigens from Neisseria meningitidis by Aston-Deaville, Sebastian, Carlsson, Emil, Saleem, Muhammad, Thistlethwaite, Angela, Chan, Hannah, Maharjan, Sunil, Facchetti, Alessandra, Feavers, Ian M., Alistair Siebert, C., Collins, Richard F., Roseman, Alan, Derrick, Jeremy P.

“…Neisseria meningitidis is the causative agent of meningococcal meningitis and sepsis and remains a significant public health problem in many countries. Efforts…”
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Model for the architecture of caveolae based on a flexible, net-like assembly of Cavin1 and Caveolin discs by Stoeber, Miriam, Schellenberger, Pascale, Siebert, C. Alistair, Leyrat, Cedric, Helenius, Ari, Grünewald, Kay

“…Caveolae are invaginated plasma membrane domains involved in mechanosensing, signaling, endocytosis, and membrane homeostasis. Oligomers of membrane-embedded…”
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Data scarce modelling the impact of present and future groundwater development on Jordan multiaquifer groundwater resources by Rödiger, T., Geyer, S., Odeh, T., Siebert, C.

“…Rapidly growing demands and climate change stresses water resources worldwide and leads to highly competitive situations between the environment and…”
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Two distinct trimeric conformations of natively membrane-anchored full-length herpes simplex virus 1 glycoprotein B by Zeev-Ben-Mordehai, Tzviya, Vasishtan, Daven, Durán, Anna Hernández, Vollmer, Benjamin, White, Paul, Pandurangan, Arun Prasad, Siebert, C. Alistair, Topf, Maya, Grünewald, Kay

“…Many viruses are enveloped by a lipid bilayer acquired during assembly, which is typically studded with one or two types of glycoproteins. These viral surface…”
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Phase 1 study of stereotactic body radiotherapy and interleukin-2--tumor and immunological responses by Seung, Steven K, Curti, Brendan D, Crittenden, Marka, Walker, Edwin, Coffey, Todd, Siebert, Janet C, Miller, William, Payne, Roxanne, Glenn, Lyn, Bageac, Alexandru, Urba, Walter J

“…Preclinical models suggest that focal high-dose radiation can make tumors more immunogenic. We performed a pilot study of stereotactic body radiation therapy…”
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Cryo-ET detects bundled triple helices but not ladders in meiotic budding yeast by Ma, Olivia X, Chong, Wen Guan, Lee, Joy K E, Cai, Shujun, Siebert, C Alistair, Howe, Andrew, Zhang, Peijun, Shi, Jian, Surana, Uttam, Gan, Lu

“…In meiosis, cells undergo two sequential rounds of cell division, termed meiosis I and meiosis II. Textbook models of the meiosis I substage called pachytene…”
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High-precision correlative fluorescence and electron cryo microscopy using two independent alignment markers by Schellenberger, Pascale, Kaufmann, Rainer, Siebert, C. Alistair, Hagen, Christoph, Wodrich, Harald, Grünewald, Kay

“…Correlative light and electron microscopy (CLEM) is an emerging technique which combines functional information provided by fluorescence microscopy (FM) with…”
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Nickel isotopic compositions of ferromanganese crusts and the constancy of deep ocean inputs and continental weathering effects over the Cenozoic by Gall, L., Williams, H.M., Siebert, C., Halliday, A.N., Herrington, R.J., Hein, J.R.

“…The global variability in nickel (Ni) isotope compositions in ferromanganese crusts is investigated by analysing surface samples of 24 crusts from various…”
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Extracellular Vesicles: A Platform for the Structure Determination of Membrane Proteins by Cryo-EM by Zeev-Ben-Mordehai, Tzviya, Vasishtan, Daven, Siebert, C. Alistair, Whittle, Cathy, Grünewald, Kay

“…Membrane protein-enriched extracellular vesicles (MPEEVs) provide a platform for studying intact membrane proteins natively anchored with the correct topology…”
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A new type of submarine chimneys built of halite by Siebert, C., Ionescu, D., Mallast, U., Merchel, S., Merkel, B., Möller, P., Pavetich, S., Pohl, T., Rödiger, T., Yechieli, Y.

“…In contrast to the subaquatic sulphide and carbonate chimneys, which are known from Mid Ocean Ridges and abyssal submarine volcanoes, chimneys built of salts…”
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Independent Mechanisms Target SMCHD1 to Trimethylated Histone H3 Lysine 9-Modified Chromatin and the Inactive X Chromosome by Brideau, Nicholas J., Coker, Heather, Gendrel, Anne-Valerie, Siebert, C. Alistair, Bezstarosti, Karel, Demmers, Jeroen, Poot, Raymond A., Nesterova, Tatyana B., Brockdorff, Neil

“…The chromosomal protein SMCHD1 plays an important role in epigenetic silencing at diverse loci, including the inactive X chromosome, imprinted genes, and the…”
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Structures of Lysenin Reveal a Shared Evolutionary Origin for Pore-Forming Proteins And Its Mode of Sphingomyelin Recognition by De Colibus, Luigi, Sonnen, Andreas F.-P., Morris, Keith J., Siebert, C. Alistair, Abrusci, Patrizia, Plitzko, Jürgen, Hodnik, Vesna, Leippe, Matthias, Volpi, Emanuela, Anderluh, Gregor, Gilbert, Robert J.C.

“…Pore-forming proteins insert from solution into membranes to create lesions, undergoing a structural rearrangement often accompanied by oligomerization…”
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Pillar data‐acquisition strategies for cryo‐electron tomography of beam‐sensitive biological samples by Parkhurst, James M., Varslot, Trond, Dumoux, Maud, Siebert, C. Alistair, Darrow, Michele, Basham, Mark, Kirkland, Angus, Grange, Michael, Evans, Gwyndaf, Naismith, James H.

“…For cryo‐electron tomography (cryo‐ET) of beam‐sensitive biological specimens, a planar sample geometry is typically used. As the sample is tilted, the…”
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Structural cycle of the Thermus thermophilus PilF ATPase: the powering of type IVa pilus assembly by Collins, Richard, Karuppiah, Vijaykumar, Siebert, C. Alistair, Dajani, Rana, Thistlethwaite, Angela, Derrick, Jeremy P.

“…Type IV pili are responsible for a diverse range of functions, including twitching motility and cell adhesion. Assembly of the pilus fiber is driven by a…”
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Computational models of amorphous ice for accurate simulation of cryo-EM images of biological samples by Parkhurst, James M., Cavalleri, Anna, Dumoux, Maud, Basham, Mark, Clare, Daniel, Siebert, C. Alistair, Evans, Gwyndaf, Naismith, James H., Kirkland, Angus, Essex, Jonathan W.

“…•Molecular dynamics simulations including the largest model of a protein in ice.•A gaussian random field approach to modelling the amorphous ice potential.•The…”
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The full-length cell–cell fusogen EFF-1 is monomeric and upright on the membrane by Zeev-Ben-Mordehai, Tzviya, Vasishtan, Daven, Siebert, C. Alistair, Grünewald, Kay

“…Fusogens are membrane proteins that remodel lipid bilayers to facilitate membrane merging. Although several fusogen ectodomain structures have been solved,…”
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