Signaling Molecules of Human Skin Cells as the Targets for Injection Cosmetology

Signaling Molecules of Human Skin Cells as the Targets for Injection Cosmetology

Skin aging is a natural process that cannot be stopped. However, there are many ways to help attenuate premature aging of the skin and reduce the signs that have already appeared. One of them is the subcutaneous administration of preparations containing a combination of hyaluronic acid, active amino...

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Bibliographic Details
Published in:Clinical, cosmetic and investigational dermatology Vol. 14; pp. 1473 - 1480
Main Authors: Gazitaeva, Zarema I, Drobintseva, Anna O, Prokopov, Aleksei Y, Sidorina, Anna N, Leonteva, Daria O, Kvetnoy, Igor M
Format: Journal Article
Language:English
Published: New Zealand Dove Medical Press Limited 01-01-2021
Taylor & Francis Ltd
Dove
Dove Medical Press
Subjects:
active amino acids
Aging
Amino acids
Antibodies
Aqueous solutions
Care and treatment
Cell culture
Cell division
clock
Collagen
Cosmetics
Cytokeratin
Fibroblasts
Gene expression
Growth factors
Hyaluronic acid
klotho
Melatonin
merkel cells
Microscopy
Original Research
Oxidative stress
Peptides
Proteins
Radiation
reparative processes
Skin
skin aging
Russia
United States > US
klotho
Merkel cells
collagen
active amino acids
skin aging
clock
reparative processes
hyaluronic acid
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Summary:Skin aging is a natural process that cannot be stopped. However, there are many ways to help attenuate premature aging of the skin and reduce the signs that have already appeared. One of them is the subcutaneous administration of preparations containing a combination of hyaluronic acid, active amino acids, and peptides providing an anti-aging clinical effect. The purpose of this research is to study in vitro new signaling molecules with the anti-aging effects and influence of hyaluronic acid fillers on its expression. The study was conducted using cell cultures of human facial skin: 1) mixed culture of human facial skin keratinocytes and fibroblasts, and 2) culture of human facial skin keratinocytes enriched with Merkel cells. Immunocytochemistry, confocal microscopy and Western blot were used to identify markers of aging. HA-Y and HA-S activated the expression of Klotho in the case of aging mixed culture of human skin keratinocytes and Merkel cells. The increase in expression of MTH-1 with aging of cultures provides evidence of activating defense mechanisms against reactive oxygen species that are accumulating with aging, under the action of HA-S and HA-Y. There was a statistically valid increase in the area of expression of melatonin receptor 1A and 1B markers when adding both HA-S and HA-Y to cultured cells. This investigation showed that the studied fillers have biological effects, testifying the stimulation of reparative processes in the skin under their control.
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ISSN:1178-7015
1178-7015
DOI:10.2147/CCID.S321104