Immuno-chemical non-cross-reactivity between eukaryotic and prokaryotic seryl-tRNA synthetases

Monospecific polyclonal antibodies (pAbs) against highly purified bovine seryl-tRNA synthetase (SerRS, EC 6.1.1.1) were prepared and their specificity tested. The interactions of pAbs with SerRS from different organisms were investigated by protein immunoblotting and ELISA methods. pAbs inhibit euka...

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Bibliographic Details
Published in:	FEBS letters Vol. 292; no. 1; pp. 76 - 78
Main Authors:	Sidorik, L.L., Gudzera, O.I., Dragovoz, V.A., Tukalo, M.A., Beresten, S.F.
Format:	Journal Article
Language:	English
Published:	Amsterdam Elsevier B.V 04-11-1991 Elsevier
Subjects:	Analytical, structural and metabolic biochemistry Animals Antibodies Biological and medical sciences Blotting, Western bovine serum albumin BSA Cattle Cross Reactions E coli Enzyme-Linked Immunosorbent Assay Enzymes and enzyme inhibitors Escherichia coli Escherichia coli - enzymology Fundamental and applied biological sciences. Psychology Immunochemical cross-reaction Immunohistochemistry Ligases Liver - enzymology Monospecific polyclonal antibody pAbs PBS phenylmethylsulphonyl fluoride PMSF rabbit monospecific polyclonal antibodies against bovine SerRS Rabbits SDS Serine-tRNA Ligase - immunology Serine-tRNA Ligase - metabolism SerRS Seryl-tRNA synthetase sodium dodecylsulphate sodium phosphate buffer SerRS PBS SDS Escherichia coli sodium phosphate buffer pAbs BSA rabbit monospecific polyclonal antibodies against bovine SerRS Seryl-tRNA synthetase E coli Immunochemical cross-reaction PMSF phenylmethylsulphonyl fluoride Monospecific polyclonal antibody bovine serum albumin sodium dodecylsulphate Bovine Enzyme Antibody Molecular evolution Vertebrata Mammalia Cross reaction Immunoblotting assay Artiodactyla ELISA assay Ungulata Comparative study
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Summary:	Monospecific polyclonal antibodies (pAbs) against highly purified bovine seryl-tRNA synthetase (SerRS, EC 6.1.1.1) were prepared and their specificity tested. The interactions of pAbs with SerRS from different organisms were investigated by protein immunoblotting and ELISA methods. pAbs inhibit eukaryotic SerRS aminoacylating activity and exert no effect on SerRS activity from prokaryotes. It is proposed that prokaryotic and eukaryotic SerRS evolve from different ancestor genes.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0014-5793 1873-3468
DOI:	10.1016/0014-5793(91)80838-T