Search Results - "Siar, El Hocine"

Effect of Concentrated Salts Solutions on the Stability of Immobilized Enzymes: Influence of Inactivation Conditions and Immobilization Protocol by Braham, Sabrina Ait, Siar, El-Hocine, Arana-Peña, Sara, Carballares, Diego, Morellon-Sterling, Roberto, Bavandi, Hossein, de Andrades, Diandra, Kornecki, Jakub F, Fernandez-Lafuente, Roberto

“…This paper aims to investigate the effects of some salts (NaCl, (NH ) SO and Na SO ) at pH 5.0, 7.0 and 9.0 on the stability of 13 different immobilized…”
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Immobilization/Stabilization of Ficin Extract on Glutaraldehyde-Activated Agarose Beads. Variables That Control the Final Stability and Activity in Protein Hydrolyses by El-Hocine Siar, Sara Arana-Peña, Oveimar Barbosa, Mohammed Nasreddine Zidoune, Roberto Fernandez-Lafuente

“…Ficin extract has been immobilized on different 4% aminated-agarose beads. Using just ion exchange, immobilization yield was poor and expressed activity did…”
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Effect of Tris Buffer in the Intensity of the Multipoint Covalent Immobilization of Enzymes in Glyoxyl-Agarose Beads by Braham, Sabrina Ait, Morellon-Sterling, Roberto, de Andrades, Diandra, Rodrigues, Rafael C., Siar, El-Hocine, Aksas, Ali, Pedroche, Justo, Millán, Maria del Carmen, Fernandez-Lafuente, Roberto

“…Tris is an extensively used buffer that presents a primary amine group on its structure. In the present work trypsin, chymotrypsin and penicillin G acylase…”
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Effect of rosemary (Rosmarinus officinalis L.) supplementation on fresh cheese: Physicochemical properties, antioxidant potential, and sensory attributes by Himed‐Idir, Hayat, Mouhoubi, Khokha, Siar, El‐hocine, Boudries, Halim, Mansouri, Hafid, Adjeroud, Nawel, Madani, Khodir, Boulekbache‐Makhlouf, Lila

“…Rosmarinus officinalis L. leaves can be valorized by their uses in the production of healthy foods. Rosemary powder and its lyophilized ethanolic extract at…”
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Use of Alcalase in the production of bioactive peptides: A review by Tacias-Pascacio, Veymar G., Morellon-Sterling, Roberto, Siar, El-Hocine, Tavano, Olga, Berenguer-Murcia, Ángel, Fernandez-Lafuente, Roberto

“…This review aims to cover the uses of the commercially available protease Alcalase in the production of biologically active peptides since 2010. Immobilization…”
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Use of glyoxyl-agarose immobilized ficin extract in milk coagulation: Unexpected importance of the ficin loading on the biocatalysts by Siar, El-Hocine, Morellon-Sterling, Roberto, Zidoune, Mohammed Nasreddine, Fernandez-Lafuente, Roberto

“…A protein extract obtained from fig tree (Ficus carica) latex containing ficin activity was immobilized on glyoxyl agarose. Different biocatalyst loadings were…”
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Amination of ficin extract to improve its immobilization on glyoxyl-agarose: Improved stability and activity versus casein by Siar, El-Hocine, Morellon-Sterling, Roberto, Zidoune, Mohammed Nasreddine, Fernandez-Lafuente, Roberto

“…Ficin extract has been aminated using ethylenediamine and carbodiimide to transform all exposed carboxylic groups into amino groups, retaining around 80% of…”
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Effect of amine length in the interference of the multipoint covalent immobilization of enzymes on glyoxyl agarose beads by Morellon-Sterling, Roberto, Siar, El-Hocine, Braham, Sabrina Ait, de Andrades, Diandra, Pedroche, Justo, Millán, Mª del Carmen, Fernandez-Lafuente, Roberto

“…•The presence of aminated compounds during the enzymes immobilization reduces the biocatalysts stability.•The decrease of the enzyme stability caused by the…”
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Glyoxyl-ficin: An example where a more intense multipoint covalent attachment may decrease enzyme stability by Siar, El-Hocine, Morellon-Sterling, Roberto, Carballares, Diego, Rocha-Martin, Javier, Barbosa, Oveimar, Bolivar, Juan M., Fernandez-Lafuente, Roberto

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Stabilization of ficin extract by immobilization on glyoxyl agarose. Preliminary characterization of the biocatalyst performance in hydrolysis of proteins by Siar, El-Hocine, Zaak, Hadjer, Kornecki, Jakub F., Zidoune, Mohammed Nasreddine, Barbosa, Oveimar, Fernandez-Lafuente, Roberto

“…[Display omitted] •A protein extract containing Ficin was immobilized on glyoxyl agarose beads.•The activity was 60% using both BANA and casein as…”
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Solid phase chemical modification of agarose glyoxyl-ficin: Improving activity and stability properties by amination and modification with glutaraldehyde by Siar, El-Hocine, Arana-Peña, Sara, Barbosa, Oveimar, Zidoune, Mohammed Nasreddine, Fernandez-Lafuente, Roberto

“…[Display omitted] •Ficin extract has been immobilized on glyoxyl agarose.•Carbodiimide mediated amination of the immobilized enzyme altered stability and…”
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Effect of immobilization rate and enzyme crowding on enzyme stability under different conditions. The case of lipase from Thermomyces lanuginosus immobilized on octyl agarose beads by Zaak, Hadjer, Siar, El-Hocine, Kornecki, Jakub F., Fernandez-Lopez, Laura, Pedrero, Sara G., Virgen-Ortíz, Jose J., Fernandez-Lafuente, Roberto

“…[Display omitted] •TLL is very rapidly immobilized on octyl agarose beads.•Apparent TLL stability increases using fully loaded biocatalyst due to diffusion…”
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Support Enzyme Loading Influences the Effect of Aldehyde Dextran Modification on the Specificity of Immobilized Ficin for Large Proteins by Siar, El Hocine, Abellanas-Perez, Pedro, Rocha-Martin, Javier, Fernandez-Lafuente, Roberto

“…It has been reported that the modification of immobilized glyoxyl-ficin with aldehyde dextran can promote steric hindrances that greatly reduce the activity of…”
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Advantages of Supports Activated with Divinyl Sulfone in Enzyme Coimmobilization: Possibility of Multipoint Covalent Immobilization of the Most Stable Enzyme and Immobilization via Ion Exchange of the Least Stable Enzyme by Morellon-Sterling, Roberto, Carballares, Diego, Arana-Peña, Sara, Siar, El-Hocine, Braham, Sabrina Ait, Fernandez-Lafuente, Roberto

“…Vinyl sulfone (VS)–agarose beads were used to develop a new strategy to coimmobilize enzymes with very different stabilities, enabling the reuse of the most…”
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Coimmobilization of enzymes in bilayers using pei as a glue to reuse the most stable enzyme: Preventing pei release during inactivated enzyme desorption by Zaak, Hadjer, Kornecki, Jakub F., Siar, El-Hocine, Fernandez-Lopez, Laura, Corberán, V. Cortés, Sassi, Mohamed, Fernandez-Lafuente, Roberto

“…[Display omitted] •Octyl-lipase coated with PEI may be used to co-immobilize lipases and galactosidase.•Desorption of the second enzyme by incubation at high…”
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Tuning Almond Lipase Features by Using Different Immobilization Supports by Oumaima Cherni, Diego Carballares, El Hocine Siar, Pedro Abellanas-Perez, Diandra de Andrades, Javier Rocha-Martin, Sellema Bahri, Roberto Fernandez-Lafuente

“…The lipase from Prunus dulcis almonds has been immobilized for the first time. For this purpose, two different supports, an octadecyl methacrylate particulate…”
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Ficin: A protease extract with relevance in biotechnology and biocatalysis by Morellon-Sterling, Roberto, El-Siar, Hocine, Tavano, Olga L., Berenguer-Murcia, Ángel, Fernández-Lafuente, Roberto

“…Due to the problems raised by the use of animal or microbial recombinant proteases, the use of proteases from vegetable origin is becoming increasingly…”
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Tailoring the specificity of ficin versus large hemoglobin and small casein by co-immobilizing inert proteins on the immobilized enzyme layer and further modification with aldehyde dextran by Siar, El Hocine, Abellanas-Perez, Pedro, Rocha-Martin, Javier, Fernandez-Lafuente, Roberto

“…Ficin has been immobilized at full loading on glyoxyl agarose beads. Then, ficin was blocked with 2,2′-dipyridyldisulfide. To be effective, the modification…”
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Designing tailor-made steric matters to improve the immobilized ficin specificity for small versus large proteins by Siar, El Hocine, Abellanas-Perez, Pedro, Morellon-Sterling, Roberto, Bolivar, Juan M., Rocha-Martin, Javier, Fernandez-Lafuente, Roberto

“…The development of strategies that can permit to adjust the size specificity of immobilized proteases by the generation of steric hindrances may enlarge its…”
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Changes in ficin specificity by different substrate proteins promoted by enzyme immobilization by Gonzalez-Vasquez, Alex D., Hocine, El Siar, Urzúa, Marcela, Rocha-Martin, Javier, Fernandez-Lafuente, Roberto

“…Ficin extract has been immobilized using different supports: glyoxyl and Aspartic/1,6 hexamethylenediamine (Asp/HA) agarose beads. The latter was later…”
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