Search Results - "Shukla, Abhinav A"

Downstream processing of monoclonal antibodies—Application of platform approaches by Shukla, Abhinav A., Hubbard, Brian, Tressel, Tim, Guhan, Sam, Low, Duncan

“…This paper presents an overview of large-scale downstream processing of monoclonal antibodies and Fc fusion proteins (mAbs). This therapeutic modality has…”
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Host cell protein clearance during protein a chromatography: Development of an improved column wash step by Shukla, Abhinav A., Hinckley, Peter

“…Host cell protein (HCP) contaminant clearance is a significant concern during downstream process development for biopharmaceuticals. Protein A chromatography…”
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High-throughput miniaturized bioreactors for cell culture process development: Reproducibility, scalability, and control by Rameez, Shahid, Mostafa, Sigma S., Miller, Christopher, Shukla, Abhinav A.

“…Decreasing the timeframe for cell culture process development has been a key goal toward accelerating biopharmaceutical development. Advanced Microscale…”
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Evolving trends in mAb production processes by Shukla, Abhinav A., Wolfe, Leslie S., Mostafa, Sigma S., Norman, Carnley

“…Monoclonal antibodies (mAbs) have established themselves as the leading biopharmaceutical therapeutic modality. The establishment of robust manufacturing…”
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Defining process design space for monoclonal antibody cell culture by Abu-Absi, Susan Fugett, Yang, LiYing, Thompson, Patrick, Jiang, Canping, Kandula, Sunitha, Schilling, Bernhard, Shukla, Abhinav A

“…The concept of design space has been taking root as a foundation of in-process control strategies for biopharmaceutical manufacturing processes. During mapping…”
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Recent advances in large-scale production of monoclonal antibodies and related proteins by Shukla, Abhinav A, Thömmes, Jörg

“…The rapid development of high-yielding and robust manufacturing processes for monoclonal antibodies is an area of significant focus in the biopharmaceutical…”
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Single-use disposable technologies for biopharmaceutical manufacturing by Shukla, Abhinav A, Gottschalk, Uwe

“…Highlights ► Single-use disposable technologies in the manufacturing of biopharmaceuticals ► Advantages and limitations of single-use manufacturing ►…”
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Multimodal chromatography: Characterization of protein binding and selectivity enhancement through mobile phase modulators by Wolfe, Leslie S., Barringer, Cartney P., Mostafa, Sigma S., Shukla, Abhinav A.

“…•We have characterized protein binding to mixed-mode chromatography resins.•We have developed novel wash sequences to remove contaminants.•We have applied…”
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A mechanistic study of Protein A chromatography resin lifetime by Jiang, Canping, Liu, Jing, Rubacha, Michael, Shukla, Abhinav A.

“…A mechanistic study into Protein A chromatographic resin lifetime limitations is presented. Binding and mass transport properties of two widely used…”
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Protein aggregation kinetics during Protein A chromatography Case study for an Fc fusion protein by SHUKLA, Abhinav A, GUPTA, Priyanka, XUEJUN HAN

“…Protein A chromatography has come to be widely adopted for large-scale purification of monoclonal antibodies and Fc fusion proteins. The low pH conditions…”
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Defining process design space for a hydrophobic interaction chromatography (HIC) purification step: Application of quality by design (QbD) principles by Jiang, Canping, Flansburg, Lisa, Ghose, Sanchayita, Jorjorian, Paul, Shukla, Abhinav A

“…The concept of design space has been taking root under the quality by design paradigm as a foundation of in‐process control strategies for biopharmaceutical…”
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A comparison of Protein A chromatographic stationary phases: Performance characteristics for monoclonal antibody purification by Liu, Zhuo, Mostafa, Sigma S., Shukla, Abhinav A.

“…Protein A chromatography remains the dominant capture step used during the downstream purification of monoclonal antibodies (mAbs). With the recent expiry of…”
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The need for innovation in biomanufacturing by Gottschalk, Uwe, Brorson, Kurt, Shukla, Abhinav A

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Improved process analytical technology for protein a chromatography using predictive principal component analysis tools by Hou, Ying, Jiang, Canping, Shukla, Abhinav A, Cramer, Steven M

“…Protein A chromatography is widely employed for the capture and purification of antibodies and Fc‐fusion proteins. Due to the high cost of protein A resins,…”
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Demonstration of Robust Host Cell Protein Clearance in Biopharmaceutical Downstream Processes by Shukla, Abhinav A., Jiang, Canping, Ma, Junfen, Rubacha, Michael, Flansburg, Lisa, Lee, Steven S.

“…Residual host cell protein impurities (HCPs) are a key component of biopharmaceutical process related impurities. These impurities need to be effectively…”
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Exploitation of the Adsorptive Properties of Depth Filters for Host Cell Protein Removal during Monoclonal Antibody Purification by Yigzaw, Yinges, Piper, Robert, Tran, Minh, Shukla, Abhinav A.

“…Depth filtration has been widely used during process scale clarification of cell culture supernatants for the removal of cells and cell debris. However, in…”
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Demonstrating β-glucan and yeast peptide clearance in biopharmaceutical downstream processes by Jiang, Canping, Scherfner, Sarah, Shukla, Abhinav A.

“…The use of yeast‐ and plant‐derived hydrolysates in cell culture production processes has sparked concerns over the potential immunogenicity risk posed by…”
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Design of a filter train for precipitate removal in monoclonal antibody downstream processing by Kandula, Sunitha, Babu, Sudha, Jin, Mi, Shukla, Abhinav A.

“…Protein A chromatography has become widely established for the preparative purification of mAbs (monoclonal antibodies). Low pH elution from Protein A columns…”
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Preparative Purification of a Recombinant Protein by Hydrophobic Interaction Chromatography: Modulation of Selectivity by the Use of Chaotropic Additives by Shukla, Abhinav A., Peterson, Joshua, Sorge, Laura, Lewis, Patsy, Thomas, Shannon, Waugh, Steve

“…Development and implementation of a chaotropic wash step following protein loading on a hydrophobic interaction chromatographic (HIC) column is described for…”
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Hydrophobic displacement chromatography of proteins by Shukla, Abhinav A., Sunasara, Khurram M., Rupp, Randall G., Cramer, Steven M.

“…Displacement chromatography of proteins was successfully carried out in both hydrophobic interaction and reversed‐phase chromatographic systems using…”
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