Search Results - "Shouming He"

Trapping and Characterization of the Reaction Intermediate in Cyclodextrin Glycosyltransferase by Use of Activated Substrates and a Mutant Enzyme by Mosi, Renee, He, Shouming, Uitdehaag, Joost, Dijkstra, Bauke W, Withers, Stephen G

“…Cyclodextrin glycosyltransferases (CGTases) catalyze the degradation of starch into linear or cyclic oligosaccharides via a glycosyl transfer reaction…”
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Differential mechanism‐based labeling and unequivocal activity assignment of the two active sites of intestinal lactase/phlorizin hydrolase by Arribas, Juan C. Díaz, Herrero, Alicia G, Martín‐Lomas, Manuel, Cañada, F. Javier, He, Shouming, Withers, Stephen G.

“…Milk lactose is hydrolysed to galactose and glucose in the small intestine of mammals by the lactase/phlorizin hydrolase complex (LPH; EC 3.2.1.108/62). The…”
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Mechanistic Analysis of Trehalose Synthase from Mycobacterium smegmatis by Zhang, Ran, Pan, Yuan T., He, Shouming, Lam, Michael, Brayer, Gary D., Elbein, Alan D., Withers, Stephen G.

“…Trehalose synthase (TreS) catalyzes the reversible interconversion of maltose and trehalose and has been shown recently to function primarily in the…”
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Active-site peptide "fingerprinting" of glycosidases in complex mixtures by mass spectrometry. Discovery of a novel retaining beta-1,4-glycanase in Cellulomonas fimi by Hekmat, Omid, Kim, Young-Wan, Williams, Spencer J, He, Shouming, Withers, Stephen G

“…New proteomics methods are required for targeting and identification of subsets of a proteome in an activity-based fashion. Here, we report the first gel-free,…”
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Intermediate trapping on a mutant retaining alpha-galactosyltransferase identifies an unexpected aspartate residue by Lairson, Luke L, Chiu, Cecilia P C, Ly, Hoa D, He, Shouming, Wakarchuk, Warren W, Strynadka, Natalie C J, Withers, Stephen G

“…Lipopolysaccharyl-alpha-1,4-galactosyltransferase C (LgtC), a glycosyltransferase family 8 alpha-1,4-galactosyltransferase from Neisseria meningitidis,…”
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A Mechanism-Based ICAT Strategy for Comparing Relative Expression and Activity Levels of Glycosidases in Biological Systems by Hekmat, Omid, He, Shouming, Warren, R. Antony J, Withers, Stephen G

“…An activity-based isotope-coded affinity tagging (AB-ICAT) strategy for proteome-wide quantitation of active retaining endoglycosidases has been developed. Two…”
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Identification of the catalytic nucleophile of the family 29 alpha-L-fucosidase from Thermotoga maritima through trapping of a covalent glycosyl-enzyme intermediate and mutagenesis by Tarling, Chris A, He, Shouming, Sulzenbacher, Gerlind, Bignon, Christophe, Bourne, Yves, Henrissat, Bernard, Withers, Stephen G

“…Fucose-containing glycoconjugates are key antigenic determinants in many biological processes. A change in expression levels of the enzymes responsible for…”
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Identification of the catalytic nucleophile in Family 42 β-galactosidases by intermediate trapping and peptide mapping: YesZ from Bacillus subtilis by Shaikh, Fathima Aidha, Müllegger, Johannes, He, Shouming, Withers, Stephen G.

“…The mechanism-based inhibitor 2,4-dinitrophenyl 2-deoxy-2-fluoro-β- d-galactopyranoside (DNP2FGal) was used to inactivate the Family 42 β-galactosidase (YesZ)…”
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Identification of the Active Site Nucleophile in Jack Bean α-Mannosidase Using 5-Fluoro-β-l-Gulosyl Fluoride by Howard, Steven, He, Shouming, Withers, Stephen G.

“…Mannosidases play a key role in the processing of glycoproteins and thus are of considerable pharmaceutical interest and indeed have emerged as targets for the…”
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Cloning, Expression, Characterization, and Nucleophile Identification of Family 3, Aspergillus nigerβ-Glucosidase by Dan, Siegel, Marton, Ira, Dekel, Mara, Bravdo, Ben-Ami, He, Shouming, Withers, Stephen G., Shoseyov, Oded

“…The β-glucosidase from Aspergillus niger (CMI CC 324262) was purified, and an N-terminal sequence and two internal sequences were determined. BglI genomic gene…”
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Mechanism of Action and Identification of Asp242 as the Catalytic Nucleophile of Vibrio furnisii N-Acetyl-β-d-glucosaminidase Using 2-Acetamido-2-deoxy-5-fluoro-α-l-idopyranosyl Fluoride by Vocadlo, David J, Mayer, Christoph, He, Shouming, Withers, Stephen G

“…The novel mechanism-based reagent 2-acetamido-2-deoxy-5-fluoro-α-l-idopyranosyl fluoride has been synthesized, and the kinetic parameters K m = 0.23 mM and k…”
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Active-site Peptide “Fingerprinting” of Glycosidases in Complex Mixtures by Mass Spectrometry by Hekmat, Omid, Kim, Young-Wan, Williams, Spencer J., He, Shouming, Withers, Stephen G.

“…New proteomics methods are required for targeting and identification of subsets of a proteome in an activity-based fashion. Here, we report the first gel-free,…”
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Active-site Peptide “Fingerprinting” of Glycosidases in Complex Mixtures by Mass Spectrometry by Omid Hekmat, Young-Wan Kim, Spencer J. Williams, Shouming He, Stephen G. Withers

“…New proteomics methods are required for targeting and identification of subsets of a proteome in an activity-based fashion. Here, we report the first gel-free,…”
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Family 39 α-l-Iduronidases and β-d-Xylosidases React through Similar Glycosyl−Enzyme Intermediates:  Identification of the Human Iduronidase Nucleophile by Nieman, Catharine E, Wong, Alexander W, He, Shouming, Hopwood, John J, Withers, Stephen G

“…The inclusion of both β-d-xylosidases and α-l-iduronidases within the same sequence-related family (family 39), despite the considerable difference in…”
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Identification of the Catalytic Nucleophile of the Family 29 α-L-Fucosidase from Thermotoga maritima through Trapping of a Covalent Glycosyl-Enzyme Intermediate and Mutagenesis by Chris A. Tarling, Shouming He, Gerlind Sulzenbacher, Christophe Bignon, Yves Bourne, Bernard Henrissat, Stephen G. Withers

“…Fucose-containing glycoconjugates are key antigenic determinants in many biological processes. A change in expression levels of the enzymes responsible for…”
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Identification of Asp-130 as the Catalytic Nucleophile in the Main α-Galactosidase from Phanerochaete chrysosporium, a Family 27 Glycosyl Hydrolase by Hart, Daniel O, He, Shouming, Chany, Calvin J, Withers, Stephen G, Sims, Paul F. G, Sinnott, Michael L, Brumer, Harry

“…Characterization of the complete gene sequence encoding the α-galactosidase from Phanerochaete chrysosporium confirms that this enzyme is a member of glycosyl…”
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The synthesis, testing and use of 5-fluoro-α- d-galactosyl fluoride to trap an intermediate on green coffee bean α-galactosidase and identify the catalytic nucleophile by Ly, Hoa D, Howard, Steven, Shum, Kelly, He, Shouming, Zhu, Alex, Withers, Stephen G

“…5-Fluoro-α- d-galactopyranosyl fluoride was synthesized and its interaction with the active site of an α-galactosidase from green coffee bean ( Coffea…”
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Assignment of Sweet Almond β-Glucosidase as a Family 1 Glycosidase and Identification of Its Active Site Nucleophile by Shouming He, Stephen G. Withers

“…Sweet almond β-glucosidase is a well studied glycosidase, having been subjected to numerous kinetic analyses and inhibition studies. However, it is not known…”
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Assignment of Sweet Almond β-Glucosidase as a Family 1 Glycosidase and Identification of Its Active Site Nucleophile by He, Shouming, Withers, Stephen G.

“…Sweet almond β-glucosidase is a well studied glycosidase, having been subjected to numerous kinetic analyses and inhibition studies. However, it is not known…”
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Identification of Asp197 as the catalytic nucleophile in the family 38 α-mannosidase from bovine kidney lysosomes by Numao, Shin, He, Shouming, Evjen, Gry, Howard, Steven, Tollersrud, Ole K., Withers, Stephen G.

“…Bovine kidney lysosomal α-mannosidase is a family 38 α-mannosidase involved in the degradation of glycoproteins. The mechanism-based reagent, 5-fluoro-β-…”
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