Search Results - "Shone, C.C."

assay for botulinum toxin types A, B and F that requires both functional binding and catalytic activities within the neurotoxin by Evans, E.R, Skipper, P.J.A, Shone, C.C

“…To develop a novel assay technique for the botulinum neurotoxin family (BoNTs) which is dependent on both the endopeptidase and receptor-binding activities of…”
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Double anchorage to the membrane and intact interchain disulfide bond are required for the low-pH-induced entry of tetanus and botulinum neurotoxins into neurons by Pirazzini, M., Rossetto, O., Bolognese, P., Shone, C.C., Montecucco, C.

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Membrane translocation of tetanus and botulinum neurotoxins by Pirazzini, M., Rossetto, O., Bordin, F., Shone, C.C., Binz, T., Montecucco, C.

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An assay for botulinum toxin types A, B and F that requires both functional binding and catalytic activities within the neurotoxin: Functional botulinum toxin assays by Evans, E.R., Skipper, P.J.A., Shone, C.C.

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Peripheral cholinergic specificity of botulinum type A neurotoxin by Colasante, C., Morbiato, L., Paoli, M., Shone, C.C., Muraro, L., Sheikh, K., Rossetto, O., Montecucco, C., Molgó, J.

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Thioredoxin and its reductase are present on synaptic vesicles and their inhibition prevents the paralysis induced by botulinum neurotoxin by Tehran, D. Azarnia, Pirazzini, M., Zanetti, G., Megighian, A., Scorzeto, M., Fillo, S., Shone, C.C., Binz, T., Rossetto, O., Lista, F., Montecucco, C.

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Botulinum Neurotoxin Type C Cleaves a Single Lys-Ala Bond within the Carboxyl-terminal Region of Syntaxins by Schiavo, Giampietro, Shone, Clifford C., Bennett, Mark K., Scheller, Richard H., Montecucco, Cesare

“…Botulinum neurotoxin serotype C (BoNT/C) is a 150-kDa protein produced by Clostridium botulinum, which causes animal botulism. In contrast to the other…”
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Botulinum neurotoxin serotype F is a zinc endopeptidase specific for VAMP/synaptobrevin by SCHIAVO, G, SHONE, C. C, ROSSETTO, O, ALEXANDER, F. C. G, MONTECUCCO, C

“…Botulinum neurotoxin serotype F contains the zinc binding motif of zinc endopeptidases. Atomic adsorption analysis of highly purified toxin preparation…”
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Development of novel assays for botulinum type A and B neurotoxins based on their endopeptidase activities by Hallis, B. (Centre for Applied Microbiology and Research, Salisbury, Wiltshire, UK.), James, B.A.F, Shone, C.C

“…A novel assay method based on the endopeptidase activities of the botulinum neurotoxins has been developed and applied to the detection of botulinum type A and…”
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Analysis of the substrate recognition domain determinants of Botulinum Type B toxin using Phage Display by Evans, E.R., Sutton, J.M., Gravett, A., Shone, C.C.

“…The botulinum neurotoxin endopeptidases appear to recognise their intracellular protein substrates via two distinct sites: the cleavage site sequence and a…”
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A role for the interchain disulfide or its participating thiols in the internalization of botulinum neurotoxin A revealed by a toxin derivative that binds to ecto-acceptors and inhibits transmitter release intracellularly by DE PAIVA, A, POULAIN, B, LAWRENCE, G. W, SHONE, C. C, TAUC, L, DOLLY, J. O

“…Botulinum neurotoxin type A consists of a disulfide-linked light and heavy chain, with an intradisulfide present within the C-terminal half of the latter. The…”
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Peptide substrate specificity and properties of the zinc-endopeptides activity of botulinum type B neurotoxin by Shone, C.C, Roberts, A.K

“…Clostridium botulinum type B neurotoxin has been shown to be a zinc endopeptidase specific for vesicle-associated membrane protein (VAMP). A synthetic peptide…”
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Heterologous combinations of heavy and light chains from botulinum neurotoxin A and tetanus toxin inhibit neurotransmitter release in Aplysia by POULAIN, B, MOCHIDA, S, WELLER, U, HOGY, B, HABERMANN, E, WADSWORTH, J. D. F, SHONE, C. C, DOLLY, J. O, TAUC, L

“…The neuroparalytic activities of botulinum neurotoxin type A (BoNT A), tetanus toxin (TeTx), or homologous and heterologous combinations of their constituent…”
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Development of in vitro assays for the detection of botulinum toxins in foods by Wictome, M, Newton, K.A, Jameson, K, Dunnigan, P, Clarke, S, Gaze, J, Tauk, A, Foster, K.A, Shone, C.C

“…Currently the only accepted method for the detection of botulinum neurotoxin in contaminated samples is the mouse bioassay. Although highly sensitive this test…”
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Botulinum neurotoxin type B. Its purification, radioiodination and interaction with rat-brain synaptosomal membranes by Evans, D.M, Williams, R.S, Shone, C.C, Hambleton, P, Melling, J, Dolly, J.O

“…Neurotoxin from Clostridium botulinum type B was purified to homogeneity by by affinity and ion-exchange chromatography; specific neurotoxicity of this protein…”
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Inactivation of Clostridium botulinum type A neurotoxin by trypsin and purification of two tryptic fragments by Shone, C.C, Hambleton, P, Melling, J

“…1 Limited treatment of Clostridium botulinum type A neurotoxin with trypsin resulted in the cleavage of the heavy (95 000 Da) subunit at approximately the…”
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