Fibroblasts cultured from venous ulcers display cellular characteristics of senescence

Fibroblasts cultured from venous ulcers display cellular characteristics of senescence

Purpose: A well-recognized characteristic of venous ulcers is impaired healing. Fibroblasts cultured from venous ulcers (wound-fb) have been shown to have reduced growth rates and are larger than normal fibroblasts (normal-fb) from the ipsilateral limb. Reduced growth capacity and morphologic change...

Full description

Saved in:
Bibliographic Details
Published in:Journal of vascular surgery Vol. 28; no. 5; pp. 876 - 883
Main Authors: Mendez, Manuel V., Stanley, Andrew, Park, Hee-Young, Shon, Karen, Phillips, Tania, Menzoian, James O.
Format: Journal Article Conference Proceeding
Language:English
Published: New York, NY Mosby, Inc 01-11-1998
Elsevier
Subjects:
beta-Galactosidase - metabolism
Biological and medical sciences
Blotting, Western
Cells, Cultured
Cellular Senescence
Chronic Disease
Dermatology
Fibroblasts - physiology
Fibronectins - metabolism
Humans
In Vitro Techniques
Medical sciences
Middle Aged
RNA, Messenger - analysis
Varicose Ulcer - pathology
Varicose Ulcer - physiopathology
Vascular disorders of the skin
Wound Healing
Human
Cell proliferation
Cell culture
Skin disease
Senescence
Pathogenesis
Lower limb
Ulcer
Cicatrization
Fibroblast
Leg
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Purpose: A well-recognized characteristic of venous ulcers is impaired healing. Fibroblasts cultured from venous ulcers (wound-fb) have been shown to have reduced growth rates and are larger than normal fibroblasts (normal-fb) from the ipsilateral limb. Reduced growth capacity and morphologic changes are 2 well-known traits of cellular senescence. Other molecular changes are overexpression of matrix proteins, such as cellular fibronectin (cFN), and enhanced activity of β-galactosidase at pH of 6.0 (senescence associated β-Gal, or SA-β-Gal). Senescence, an irreversible arrest of cell proliferation with maintenance of metabolic functions, may represent in vivo aging and thus may be related to impaired healing. Methods: Cultured normal-fb and wound-fb from 7 venous ulcer patients (average age, 51 years) were obtained by taking punch biopsies of the perimeter of the ulcer and from the ipsilateral thigh of the same patient. Growth rates, SA-β-Gal activity, and level of cFN protein (immunoblot) and message (Northern blot) were measured. Results: In all patients, wound-fb growth rates were significantly lower than those of normal-fb ( P = .006). A higher percentage of SA-β-Gal positive cells were found in all wound-fb (average, 6.3% vs. 0.21%; P = .016). The level of cFN, was consistently higher in all wound-fb tested. Also, in 4 patients, the level of cFN messenger RNA (mRNA) was increased. Conclusion: Fibroblasts cultured from venous ulcers exhibited characteristics associated with senescent cells. Accumulation of senescent cell in ulcer environment may be associated with impaired healing. (J Vasc Surg 1998;28:876-83.)
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0741-5214
1097-6809
DOI:10.1016/S0741-5214(98)70064-3