Assessment of bacteriocin production by clinical Pseudomonas aeruginosa isolates and their potential as therapeutic agents

Bacterial infections and the rising antimicrobial resistance pose a significant threat to public health. Pseudomonas aeruginosa produces bacteriocins like pyocins, especially S-type pyocins, which are promising for biological applications. This research focuses on clinical P. aeruginosa isolates to...

Full description

Saved in:

Bibliographic Details
Published in:	Microbial cell factories Vol. 23; no. 1; pp. 175 - 16
Main Authors:	Charkhian, Hamed, Soleimannezhadbari, Ehsan, Bodaqlouei, Amin, Lotfollahi, Lida, Lotfi, Hajie, Yousefi, Nesa, Shojadel, Ehsan, Gholinejad, Zafar
Format:	Journal Article
Language:	English
Published:	England BioMed Central Ltd 13-06-2024 BioMed Central BMC
Subjects:	Anti-Bacterial Agents - biosynthesis Anti-Bacterial Agents - pharmacology Antibacterial agents Antimicrobial pitied Bacterial toxins Bacteriocin Bacteriocins - biosynthesis Bacteriocins - metabolism Bacteriocins - pharmacology Chemical properties Drug resistance in microorganisms Gene expression Genetic aspects Health aspects Humans Infection Microbial Sensitivity Tests Microbiological research Mitomycin Peptides Physiological aspects Pseudomonas aeruginosa Pseudomonas aeruginosa - drug effects Pseudomonas aeruginosa - genetics Pseudomonas aeruginosa - metabolism Pseudomonas Infections - drug therapy Pseudomonas Infections - microbiology Public health Pyocins - biosynthesis Pyocins - metabolism Pyocins - pharmacology qRT-PCR S-type pyocin Iran Pseudomonas aeruginosa S-type pyocin Antimicrobial pitied Bacteriocin qRT-PCR
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	Bacterial infections and the rising antimicrobial resistance pose a significant threat to public health. Pseudomonas aeruginosa produces bacteriocins like pyocins, especially S-type pyocins, which are promising for biological applications. This research focuses on clinical P. aeruginosa isolates to assess their bacteriocin production, inhibitory spectrum, chemical structure, antibacterial agents, and preservative potential. The identification of P. aeruginosa was conducted through both phenotypic and molecular approaches. The inhibitory spectrum and antibacterial potential of the isolates were assessed. The kinetics of antibacterial peptide production were investigated, and the activity of bacteriocin was quantified in arbitrary units (AU ml ). Physico-chemical characterization of the antibacterial peptides was performed. Molecular weight estimation was carried out using SDS-PAGE. qRT-PCR analysis was employed to validate the expression of the selected candidate gene. The antibacterial activity of P. aeruginosa was attributed to the secretion of bacteriocin compounds, which belong to the S-type pyocin family. The use of mitomycin C led to a significant 65.74% increase in pyocin production by these isolates. These S-type pyocins exhibited the ability to inhibit the growth of both Gram-negative (P. mirabilis and P. vulgaris) and Gram-positive (S. aureus, S. epidermidis, E. hirae, S. pyogenes, and S. mutans) bacteria. The molecular weight of S-type pyocin was 66 kDa, and its gene expression was confirmed through qRT-PCR. These findings suggest that S-type pyocin hold significant potential as therapeutic agents against pathogenic strains. The Physico-chemical resistance of S-type pyocin underscores its potential for broad applications in the pharmaceutical, hygiene, and food industries.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1475-2859 1475-2859
DOI:	10.1186/s12934-024-02450-w