Determination of Biogas Producers in Antibiotic-Containing Sewage

Determination of Biogas Producers in Antibiotic-Containing Sewage

Treating sewage containing antibiotics is a complex process that needs research. An anaerobic method for organic matter removal from wastewater is the most optimal treatment option, which can provide antibiotic removal under the condition of prior adaptation of anaerobic microorganism association to...

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Bibliographic Details
Published in:Water, air, and soil pollution Vol. 231; no. 8
Main Authors: Golub, N. B., Shinkarchuk, M. V., Kozlovets, O. A., Morgun, B. V., Lakhneko, O. R., Stepanenko, A. I., Borisjuk, M. V.
Format: Journal Article
Language:English
Published: Cham Springer International Publishing 16-08-2020
Springer
Springer Nature B.V
Subjects:
Adaptation
Anaerobic conditions
Anaerobic microorganisms
Anaerobic processes
Anaerobic treatment
Anoxic conditions
Antibiotics
Atmospheric Protection/Air Quality Control/Air Pollution
Biogas
Biomass energy
Climate Change/Climate Change Impacts
Composition
Deoxyribonucleic acid
DNA
Drug resistance in microorganisms
Earth and Environmental Science
Environment
Environmental monitoring
Ethylenediaminetetraacetic acid
Gas chromatography
Genes
Hydrogeology
Methanogenic bacteria
Microorganisms
Norfloxacin
Nucleotide sequence
Nutrient concentrations
Organic matter
PCR
Polymerase chain reaction
Purification
Raw materials
Removal
Sewage
Sewage treatment
Soil Science & Conservation
Tetracycline
Tetracyclines
Wastewater
Wastewater treatment
Water Quality/Water Pollution
Biogas
Antibiotics
Norfloxacin
PCR
Anaerobic treatment
Tetracycline
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Summary:Treating sewage containing antibiotics is a complex process that needs research. An anaerobic method for organic matter removal from wastewater is the most optimal treatment option, which can provide antibiotic removal under the condition of prior adaptation of anaerobic microorganism association to antibiotic. The aims of the study were to obtain a microorganism association tolerant to antibiotics and to investigate the anaerobic association (methanogens) composition before and after adaptation. The subject of the study was the determination of microorganism association properties in conditions of high antibiotic concentration, which enables the determination of the adaptation mechanism. Adaptation of microorganism association to antibiotics was carried out by a gradual increase of antibiotic concentration in the nutrient medium (anaerobic conditions of detention, mesophilic regime). The qualitative and quantitative composition of biogas was determined by gas chromatography. DNA of microorganism associations was isolated by the agrobacteria DNA isolation method. The determination of archaeal 16SrRNA genes in DNA was performed by polymerase chain reaction (PCR). Information about gene structure was obtained by sequencing. It has been shown that the biogas output during anaerobic sewage treatment with antibiotic-adapted microorganism association increases regardless of antibiotic concentration. When enriching microorganism association with methanogens, tolerant to antibiotics, the biogas output grows by 32–38% depending on their amount. It was found that, when using tetracycline and norfloxacin, in the microorganism association methanogens of the genus Methanobacterium are dominant due to their resistance. Influence of antibiotics tetracycline and norfloxacin on raw materials leads to a decrease in the species volume of the Methanosarcinaceae family.
ISSN:0049-6979
1573-2932
DOI:10.1007/s11270-020-04805-6