Introduction to Time-Resolved Spectroscopy: Nanosecond Transient Absorption and Time-Resolved Fluorescence of Eosin B

Here we present a new undergraduate laboratory that will introduce the concepts of time-resolved spectroscopy and provide insight into the natural time scales on which chemical dynamics occur through direct measurement. A quantitative treatment of the acquired data will provide a deeper understandin...

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Bibliographic Details
Published in:	Journal of chemical education Vol. 95; no. 5; pp. 864 - 871
Main Authors:	Farr, Erik P, Quintana, Jason C, Reynoso, Vanessa, Ruberry, Josiah D, Shin, Wook R, Swartz, Kevin R
Format:	Journal Article
Language:	English
Published:	Easton American Chemical Society and Division of Chemical Education, Inc 08-05-2018 Division of Chemical Education, Inc and ACS Publications Division of the American Chemical Society American Chemical Society
Subjects:	Chemistry College Science College students Data acquisition Electronic structure Fluorescence Hands on Science Kinetics Laboratory Experiments Learning Light sources Molecular chemistry Molecular Structure Organic chemistry Phosphorescence Photodiode detectors Photodiodes Quantum mechanics Science Experiments Science Laboratories Scientific apparatus & instruments Signal delay Spectroscopy Spectrum analysis Time Troubleshooting Undergraduate Study California (Los Angeles) Hands-On Learning/Manipulatives Spectroscopy Instrumental Methods Upper-Division Undergraduate Laboratory Instruction Fluorescence Spectroscopy Physical Chemistry Kinetics Molecular Mechanics/Dynamics
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Summary:	Here we present a new undergraduate laboratory that will introduce the concepts of time-resolved spectroscopy and provide insight into the natural time scales on which chemical dynamics occur through direct measurement. A quantitative treatment of the acquired data will provide a deeper understanding of the role of quantum mechanics and various phenomenological expressions in predicting kinetic rates for fluorescence, phosphorescence, and nonradiative decay mechanisms. This laboratory framework focuses specifically on spectroscopy in the nanosecond regimeassisted by various steady-state spectroscopic techniquesin order to fully characterize the electronic structure and the picosecond-to-microsecond dynamics of the dye eosin B. There is great flexibility in both the recommended lab duration (1 week to several months) and course level (upper-division to graduate) due to the numerous additional experiments that may be performed at varying levels of difficulty. The necessary components include pump and probe light sources, photodiode detectors, a programmable signal delay generator, and an oscilloscope for measurements with requisite resolution. The cost of building this experiment from scratch is less than $20,000 at the time of publication, but costs are expected to decrease over time and alternate excitation sources are available. Although this lab requires some expertise with optical spectroscopy to initially build and troubleshoot, use by students has been a straightforward and valuable experience.
ISSN:	0021-9584 1938-1328
DOI:	10.1021/acs.jchemed.7b00941