Comparison of cytokine expression in mesenchymal stem cells from human placenta, cord blood, and bone marrow

Comparison of cytokine expression in mesenchymal stem cells from human placenta, cord blood, and bone marrow

Mesenchymal stem cells (MSCs) are capable of self-renewal and differentiation into lineages of mesenchymal tissues that are currently under investigation for a variety of therapeutic applications. The purpose of this study was to compare cytokine gene expression in MSCs from human placenta, cord blo...

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Bibliographic Details
Published in:Journal of Korean medical science Vol. 24; no. 4; pp. 547 - 554
Main Authors: Hwang, Jong Ha, Shim, Soung Shin, Seok, Oye Sun, Lee, Hang Young, Woo, Sang Kyu, Kim, Bong Hui, Song, Hae Ryong, Lee, Jae Kwan, Park, Yong Kyun
Format: Journal Article
Language:English
Published: Korea (South) The Korean Academy of Medical Sciences 01-08-2009
대한의학회
Subjects:
Bone Marrow Cells - cytology
Cytokines - genetics
Cytokines - metabolism
Female
Fetal Blood - cytology
Gene Expression Profiling
Humans
Mesenchymal Stromal Cells - cytology
Mesenchymal Stromal Cells - metabolism
Original
Placenta - cytology
Pregnancy
Protein Array Analysis
의학일반
Placenta
Cytokines
Bone Marrow
Fetal Blood
Mesenchymal Stem Cells
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Summary:Mesenchymal stem cells (MSCs) are capable of self-renewal and differentiation into lineages of mesenchymal tissues that are currently under investigation for a variety of therapeutic applications. The purpose of this study was to compare cytokine gene expression in MSCs from human placenta, cord blood (CB) and bone marrow (BM). The cytokine expression profiles of MSCs from BM, CB and placenta (amnion, decidua) were compared by proteome profiler array analysis. The cytokines that were expressed differently, in each type of MSC, were analyzed by real-time PCR. We evaluated 36 cytokines. Most types of MSCs had a common expression pattern including MIF (GIF, DER6), IL-8 (CXCL8), Serpin E1 (PAI-1), GROalpha(CXCL1), and IL-6. MCP-1, however, was expressed in both the MSCs from the BM and the amnion. sICAM-1 was expressed in both the amnion and decidua MSCs. SDF-1 was expressed only in the BM MSCs. Real-time PCR demonstrated the expression of the cytokines in each of the MSCs. The MSCs from bone marrow, placenta (amnion and decidua) and cord blood expressed the cytokines differently. These results suggest that cytokine induction and signal transduction are different in MSCs from different tissues.
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content type line 23
G704-000345.2009.24.4.016
http://kmbase.medric.or.kr/Main.aspx?d=KMBASE&m=VIEW&i=0191120090240040547
ISSN:1011-8934
1598-6357
DOI:10.3346/jkms.2009.24.4.547