Isolation and some properties of a strain of the iron-oxidizing bacterium Thiobacillus ferrooxidans resistant to 2,4-dinitrophenol

Among two hundred strains of iron-oxidizing bacteria isolated from the natural environment, only one, Thiobacillus ferroxidans NASF-1, grew in an Fe 2+ medium supplemented with 10 μM of 2,4-dinitrophenol (2,4-DNP). In contrast, growth of T. ferroxidans AP19-3, a strain representative of iron-oxidizi...

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Published in:	Journal of fermentation and bioengineering Vol. 86; no. 1; pp. 134 - 137
Main Authors:	Sugio, Tsuyoshi, Fujioka, Akira, Tsuchiya, Mikio, Shibusawa, Naoki, Iwahori, Kenji, Kamimura, Kazuo
Format:	Journal Article
Language:	English
Published:	Osaka Elsevier B.V 01-01-1998 Society for Fermentation and Bioengineering
Subjects:	4-dinitrophenol BACTERIA Biological and medical sciences Biotechnology FER Fundamental and applied biological sciences. Psychology HIERRO IRON iron-oxidizing bacterium ISOLATION TECHNIQUES Methods. Procedures. Technologies Microbial engineering. Fermentation and microbial culture technology OXIDACION OXIDATION OXYDATION RESISTANCE AUX PRODUITS CHIMIQUES RESISTANCE TO CHEMICALS RESISTENCIA A PRODUCTOS QUIMICOS TECHNIQUE DE L'ISOLEMENT TECNICAS DE AISLAMIENTO Thiobacillus ferrooxidans uncoupler iron-oxidizing bacterium uncoupler 2 Thiobacillus ferrooxidans 4-dinitrophenol Resistance Enzymatic activity Industrial application Iron II Bacteria Bacterial leaching Oxidation Uncoupler Comparative study
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Summary:	Among two hundred strains of iron-oxidizing bacteria isolated from the natural environment, only one, Thiobacillus ferroxidans NASF-1, grew in an Fe 2+ medium supplemented with 10 μM of 2,4-dinitrophenol (2,4-DNP). In contrast, growth of T. ferroxidans AP19-3, a strain representative of iron-oxidizing bacteria sensitive to 2,4-DNP, was strongly inhibited by 2,4-DNP at 1.0 μM, and completely repressed at 10 μM. Strain NASF-1 was also resistant to an other uncoupler, carbonylcyanide m-chlorophenylhydrazone (CCCP). The iron-oxidizing activities of strains AP19-3 and NASF-1 were respectively inhibited 69 and 23% by 10 μM 2,4-DNP, while the 14CO 2 uptake activities of the two strains were inhibited 31 and 5% by 2,4-DNP at 1.0 μM, and 100 and 89% at 5.0 μM. When intact cells of strain AP19-3 were incubated with Fe 2+ and 1.0 μM 2,4-DNP for 10 min at pH 3.0, 49 pmol 2,4-DNP per mg of protein was incorporated into the cells. In contrast, strain NASF-1 did not incorporate 2,4-DNP under the same conditions, suggesting that, compared with strain NASF-1, strain AP19-3 has a plasma membrane through which 2,4-DNP is more easily incorporated in the cytosol.
Bibliography:	1998007520 F60 ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23
ISSN:	0922-338X
DOI:	10.1016/S0922-338X(98)80047-0