Search Results - "Shibata, Norito"

RNA Interference in Planarians: Feeding and Injection of Synthetic dsRNA by Shibata, Norito, Agata, Kiyokazu

“…RNA interference (RNAi) is one of the simplest and easiest methods for specifically perturbing gene function in an organism. In planarian research, RNAi is one…”
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What is the role of PIWI family proteins in adult pluripotent stem cells? Insights from asexually reproducing animals, planarians by Kashima, Makoto, Agata, Kiyokazu, Shibata, Norito

“…Planarians have a remarkable regenerative ability owing to their adult pluripotent stem cells (aPSCs), which are called “neoblasts.” Planarians maintain a…”
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Migratory regulation by MTA homologous genes is essential for the uniform distribution of planarian adult pluripotent stem cells by Sato, Yuki, Shibata, Norito, Hashimoto, Chikara, Agata, Kiyokazu

“…The migration of adult stem cells in vivo is an important issue, but the complex tissue structures involved, and limited accessibility of the cells hinder a…”
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Loss of plac8 expression rapidly leads pluripotent stem cells to enter active state during planarian regeneration by Lee, Hayoung, Hikasa, Kanon, Umesono, Yoshihiko, Hayashi, Tetsutaro, Agata, Kiyokazu, Shibata, Norito

“…The regenerative ability of planarians relies on their adult pluripotent stem cell population. Although all stem cells express a piwi homolog, recently it has…”
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RNA interference by feeding in vitro–synthesized double‐stranded RNA to planarians: Methodology and dynamics by Rouhana, Labib, Weiss, Jennifer A., Forsthoefel, David J., Lee, Hayoung, King, Ryan S., Inoue, Takeshi, Shibata, Norito, Agata, Kiyokazu, Newmark, Phillip A.

“…Background The ability to assess gene function is essential for understanding biological processes. Currently, RNA interference (RNAi) is the only technique…”
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Searching for non‐transposable targets of planarian nuclear PIWI in pluripotent stem cells and differentiated cells by Kashima, Makoto, Agata, Kiyokazu, Shibata, Norito

“…Nuclear PIWIs together with their guide RNAs (piRNAs) epigenetically silence various genes including transposons in many organisms. In planarians, the nuclear…”
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Cellular and molecular dissection of pluripotent adult somatic stem cells in planarians by Shibata, Norito, Rouhana, Labib, Agata, Kiyokazu

“…Freshwater planarians, Plathelminthes, have been an intriguing model animal of regeneration studies for more than 100 years. Their robust regenerative ability…”
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Inheritance of a Nuclear PIWI from Pluripotent Stem Cells by Somatic Descendants Ensures Differentiation by Silencing Transposons in Planarian by Shibata, Norito, Kashima, Makoto, Ishiko, Taisuke, Nishimura, Osamu, Rouhana, Labib, Misaki, Kazuyo, Yonemura, Shigenobu, Saito, Kuniaki, Siomi, Haruhiko, Siomi, Mikiko C., Agata, Kiyokazu

“…Differentiation of pluripotent stem cells (PSCs) requires transposon silencing throughout the process. PIWIs, best known as key factors in germline transposon…”
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Volume X-Ray Micro-Computed Tomography Analysis of the Early Cephalized Central Nervous System in a Marine Flatworm, Stylochoplana pusilla by Ikenaga, Takanori, Kobayashi, Aoshi, Takeuchi, Akihisa, Uesugi, Kentaro, Maezawa, Takanobu, Shibata, Norito, Sakamoto, Tatsuya, Sakamoto, Hirotaka

“…Platyhelminthes are a phylum of simple bilaterian invertebrates with prototypic body systems. Compared with non-bilaterians such as cnidarians, the bilaterians…”
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ERK signaling controls blastema cell differentiation during planarian regeneration by Tasaki, Junichi, Shibata, Norito, Nishimura, Osamu, Itomi, Kazu, Tabata, Yoshimichi, Son, Fuyan, Suzuki, Nobuko, Araki, Ryoko, Abe, Masumi, Agata, Kiyokazu, Umesono, Yoshihiko

“…The robust regenerative ability of planarians depends on a population of somatic stem cells called neoblasts, which are the only mitotic cells in adults and…”
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Heterogeneity of chromatoid bodies in adult pluripotent stem cells of planarian Dugesia japonica by Kashima, Makoto, Kumagai, Nobuyoshi, Agata, Kiyokazu, Shibata, Norito

“…The robust regenerative ability of planarians is known to be dependent on adult pluripotent stem cells called neoblasts. One of the morphological features of…”
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Role of c‐Jun N‐terminal kinase activation in blastema formation during planarian regeneration by Tasaki, Junichi, Shibata, Norito, Sakurai, Toshihide, Agata, Kiyokazu, Umesono, Yoshihiko

“…The robust regenerative abilities of planarians absolutely depend on a unique population of pluripotent stem cells called neoblasts, which are the only mitotic…”
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DjCBC‐1, a conserved DEAD box RNA helicase of the RCK/p54/Me31B family, is a component of RNA‐protein complexes in planarian stem cells and neurons by Yoshida‐Kashikawa, Maki, Shibata, Norito, Takechi, Katsuaki, Agata, Kiyokazu

“…The stem cells of planarians, known as neoblasts, can give rise to all cell types in planarians. Neoblasts can be identified by electron microscopy as cells…”
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Identification and origin of the germline stem cells as revealed by the expression of nanos‐related gene in planarians by Sato, Kimihiro, Shibata, Norito, Orii, Hidefumi, Amikura, Reiko, Sakurai, Takashige, Agata, Kiyokazu, Kobayashi, Satoru, Watanabe, Kenji

“…The planarian's remarkable regenerative ability is thought to be supported by the stem cells (neoblasts) found throughout its body. Here we report the…”
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Isolation of planarian X‐ray‐sensitive stem cells by fluorescence‐activated cell sorting by Hayashi, Tetsutaro, Asami, Maki, Higuchi, Sayaka, Shibata, Norito, Agata, Kiyokazu

“…The remarkable capability of planarian regeneration is mediated by a group of adult stem cells referred to as neoblasts. Although these cells possess many…”
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The planarian P2X homolog in the regulation of asexual reproduction by Sakurai, Toshihide, Lee, Hayoung, Kashima, Makoto, Saito, Yumi, Hayashi, Tetsutaro, Kudome-Takamatsu, Tomomi, Nishimura, Osamu, Agata, Kiyokazu, Shibata, Norito

“…The growth in size of freshwater planarians in response to nutrient intake is limited by the eventual separation of tail and body fragments in a process called…”
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Single‐cell gene profiling of planarian stem cells using fluorescent activated cell sorting and its “index sorting” function for stem cell research by Hayashi, Tetsutaro, Shibata, Norito, Okumura, Ryo, Kudome, Tomomi, Nishimura, Osamu, Tarui, Hiroshi, Agata, Kiyokazu

“…To achieve an integrated understanding of the stem cell system of planarians at both the cellular and molecular levels, we developed a new method by combining…”
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Characterization and categorization of fluorescence activated cell sorted planarian stem cells by ultrastructural analysis by Higuchi, Sayaka, Hayashi, Tetsutaro, Hori, Isao, Shibata, Norito, Sakamoto, Hiroshi, Agata, Kiyokazu

“…Planarians have regenerative ability made possible by pluripotent stem cells referred to as neoblasts. Classical ultrastructural studies have indicated that…”
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Mouse RanBPM is a partner gene to a germline specific RNA helicase, mouse vasa homolog protein by Shibata, Norito, Tsunekawa, Naoki, Okamoto-Ito, Shino, Akasu, Ryuko, Tokumasu, Ako, Noce, Toshiaki

“…Germ cell‐specific ATP‐dependent RNA helicase, the product of the mouse vasa homolog (Mvh), has been shown to play an essential role in the development of the…”
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Different requirements for conserved post-transcriptional regulators in planarian regeneration and stem cell maintenance by Rouhana, Labib, Shibata, Norito, Nishimura, Osamu, Agata, Kiyokazu

“…Planarian regeneration depends on the presence and precise regulation of pluripotent adult somatic stem cells named neoblasts, which differentiate to replace…”
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