Search Results - "Shiba, Akio"

TAMRA/TAMRA Fluorescence Quenching Systems for the Activity Assay of Alkaline Phosphatase by Shiba, Akio, Kinoshita-Kikuta, Emiko, Kinoshita, Eiji, Koike, Tohru

“…We introduce two types of fluorescence-quenching assay for alkaline phosphatases (APs) by using a carboxytetramethyl-rhodamine (TAMRA)-labeled…”
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Profiling of protein thiophosphorylation by Phos-tag affinity electrophoresis: Evaluation of adenosine 5′-O-(3-thiotriphosphate) as a phosphoryl donor in protein kinase reactions by Kinoshita, Eiji, Kinoshita-Kikuta, Emiko, Shiba, Akio, Edahiro, Keisuke, Inoue, Yuki, Yamamoto, Kaneyoshi, Yoshida, Myu, Koike, Tohru

“…Adenosine 5′‐O‐(3‐thiotriphosphate) (ATPγS) has been widely used as a phosphoryl donor to trace protein kinase activities. However, the question remains…”
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A novel thiol-affinity micropipette tip method using zinc(II)–cyclen-attached agarose beads for enrichment of cysteine-containing molecules by Kusamoto, Hiroshi, Shiba, Akio, Koretake, Norinao, Fujioka, Haruto, Hieda, Yuhzo, Kinoshita-Kikuta, Emiko, Kinoshita, Eiji, Koike, Tohru

“…•A novel thiol-affinity micropipette tip method was developed.•Thiol compounds captured by the thiol-affinity micropipette tip are stable under air.•The…”
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