Search Results - "Shi, Lichi"
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Tracing an allosteric pathway regulating the activity of the HslV protease
Published in Proceedings of the National Academy of Sciences - PNAS (11-02-2014)“…The HslU–HslV complex functions as a bacterial proteasome, degrading substrate polypeptides to preserve cellular homeostasis. Here, we use methyl-Transverse…”
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2
Proton-Detected Solid-State NMR Reveals Intramembrane Polar Networks in a Seven-Helical Transmembrane Protein Proteorhodopsin
Published in Journal of the American Chemical Society (02-11-2011)“…We used high-resolution proton-detected multidimensional NMR to study the solvent-exposed parts of a seven-helical integral membrane proton pump,…”
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3
Conformation of a Seven-Helical Transmembrane Photosensor in the Lipid Environment
Published in Angewandte Chemie (International ed.) (07-02-2011)“…Break on through: High‐resolution multidimensional solid‐state NMR spectroscopy was used to refine the molecular conformation of a seven‐helical transmembrane…”
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4
Cyanobacterial light-driven proton pump, gloeobacter rhodopsin: complementarity between rhodopsin-based energy production and photosynthesis
Published in PloS one (27-10-2014)“…A homologue of type I rhodopsin was found in the unicellular Gloeobacter violaceus PCC7421, which is believed to be primitive because of the lack of thylakoids…”
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5
Three-Dimensional Solid-State NMR Study of a Seven-Helical Integral Membrane Proton Pump—Structural Insights
Published in Journal of molecular biology (06-03-2009)“…Proteorhodopsin (PR) is a recently discovered ubiquitous eubacterial retinal-binding light-driven proton pump. Almost 1000 PR variants are widely distributed…”
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6
The Photocycle and Proton Translocation Pathway in a Cyanobacterial Ion-Pumping Rhodopsin
Published in Biophysical journal (18-02-2009)“…The genome of thylakoidless cyanobacterium Gloeobacter violaceus encodes a fast-cycling rhodopsin capable of light-driven proton transport. We characterize the…”
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Solid-state NMR study of proteorhodopsin in the lipid environment: Secondary structure and dynamics
Published in Biochimica et biophysica acta (01-12-2009)“…Proteorhodopsins are typical retinal-binding light-driven proton pumps of heptahelical architecture widely distributed in marine and freshwater bacteria…”
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Leptosphaeria Rhodopsin: Bacteriorhodopsin-Like Proton Pump from a Eukaryote
Published in Proceedings of the National Academy of Sciences - PNAS (10-05-2005)“…Bacteriorhodopsin-like proteins provide archaea and eubacteria with a unique bioenergetic pathway comprising light-driven transmembrane proton translocation by…”
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9
Uniform isotope labeling of a eukaryotic seven-transmembrane helical protein in yeast enables high-resolution solid-state NMR studies in the lipid environment
Published in Journal of biomolecular NMR (01-02-2011)“…Overexpression of isotope-labeled multi-spanning eukaryotic membrane proteins for structural NMR studies is often challenging. On the one hand, difficulties…”
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Site-Specific Solid-State NMR Detection of Hydrogen-Deuterium Exchange Reveals Conformational Changes in a 7-Helical Transmembrane Protein
Published in Biophysical journal (03-08-2011)“…Solid-state NMR spectroscopy is an efficient tool for following conformational dynamics of membrane proteins at atomic resolution. We used this technique for…”
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Cytoplasmic Shuttling of Protons in Anabaena Sensory Rhodopsin: Implications for Signaling Mechanism
Published in Journal of molecular biology (05-05-2006)“…It was found recently that Anabaena sensory rhodopsin (ASR), which possibly serves as a photoreceptor for chromatic adaptation, interacts with a soluble…”
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12
Structural basis of diversification of fungal retinal proteins probed by site-directed mutagenesis of Leptosphaeria rhodopsin
Published in FEBS letters (29-05-2007)“…Numerous fungal genomes encode homologs of bacteriorhodopsin (BR), but only two fungal rhodopsins were overexpressed and characterized spectroscopically…”
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13
Conformational Coupling between the Cytoplasmic Carboxylic Acid and the Retinal in a Fungal Light-Driven Proton Pump
Published in Biochemistry (Easton) (26-12-2006)“…Many fungal rhodopsins, eukaryotic structural homologues of the archaeal light-driven proton pump bacteriorhodopsin, have been discovered in the course of…”
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Conformation of a Seven-Helical Transmembrane Photosensor in the Lipid Environment
Published in Angewandte Chemie (07-02-2011)“…Hochaufgelöste mehrdimensionale Festkörper‐NMR‐Spektroskopie wurde zur verbesserten Konformationsaufklärung eines aus sieben Helices bestehenden…”
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15
Solid-state NMR spectroscopy structure determination of a lipid-embedded heptahelical membrane protein
Published in Nature methods (01-10-2013)“…A strategy, based on solid-state NMR spectroscopy, for determining the structure of an oligomeric, seven-helix membrane protein ( Anabaena sensory rhodopsin)…”
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Tracing an allosteric pathway regulating the activity of the HsIV protease
Published in Proceedings of the National Academy of Sciences - PNAS (11-02-2014)“…The HsIU–HsIV complex functions as a bacterial proteasome, degrading substrate polypeptides to preserve cellular homeostasis. Here, we use methyl-Transverse…”
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17
Magic angle spinning solid-state NMR experiments for structural characterization of proteins
Published in Methods in molecular biology (Clifton, N.J.) (2012)“…Solid-state nuclear magnetic resonance (SSNMR) has become a prominent method in biology and is suitable for the characterization of insoluble proteins and…”
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Induced Secondary Structure and Polymorphism in an Intrinsically Disordered Structural Linker of the CNS: Solid-State NMR and FTIR Spectroscopy of Myelin Basic Protein Bound to Actin
Published in Biophysical journal (07-01-2009)“…The 18.5 kDa isoform of myelin basic protein (MBP) is a peripheral membrane protein that maintains the structural integrity of the myelin sheath of the central…”
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Interresidue carbonyl–carbonyl polarization transfer experiments in uniformly 13C, 15N-labeled peptides and proteins
Published in Journal of magnetic resonance (1997) (01-03-2010)“…In this work, we demonstrate that Homonuclear Rotary Resonance Recoupling (HORROR) can be used to reintroduce carbonyl–carbonyl interresidue dipolar…”
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Cyanobacterial Light-Driven Proton Pump, Gloeobacter Rhodopsin: Complementarity between Rhodopsin-Based Energy Production and Photosynthesis: e110643
Published in PloS one (01-10-2014)“…A homologue of type I rhodopsin was found in the unicellular Gloeobacter violaceus PCC7421, which is believed to be primitive because of the lack of thylakoids…”
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