Search Results - "Shershov, V E"

A Comparative Behavior Analysis of Cy5-Pyrimidine Nucleotides in Rolling Circle Amplification by Lapa, S. A., Chirkova, P. A., Surzhikov, S. A., Kuznetsova, V. E., Shershov, V. E., Chudinov, A. V.

“…Objective: The introduction of fluorescently labeled nucleotides in the process of DNA amplification in molecular genetic analysis is a very attractive…”
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Species-Level Identification of SARS-CoV-2 by an E Gene Conservative Site by Lapa, S. A., Shingareva, A. A., Faizuloev, E. B., Ammour, Yu. I., Shershov, V. E., Chudinov, A. V.

“…— The appearance of mutations in the genes encoding the surface proteins of the new type of coronavirus SARS-CoV-2, when it circulates in the host population,…”
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Generation of Active Amino Groups on the Surface of a Polyethylene Terephthalate Film and Their Quantitative Evaluation by Shtylev, G. F., Shishkin, I. Yu, Lapa, S. A., Shershov, V. E., Barsky, V. E., Polyakov, S. A., Vasiliskov, V. A., Zasedateleva, O. A., Kuznetsova, V. E., Chudinov, A. V.

“…Objective: Polyethylene terephthalate (PET) is thermally stable, biocompatible, transparent in visible and near-infrared light. The study of grafting…”
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Recombinase Polymerase Amplification for Rapid Detection of Human Bacterial Pneumonia Pathogens by Lapa, S. A., Surzhikov, S. A., Blagodatskikh, S. A., Shershov, V. E., Chudinov, A. V.

“…A diagnostic system based on recombinase polymerase amplification (RPA) has been developed to identify six bacterial pathogens of human pneumonia…”
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Immobilization of Protein Probes on Biochips with Brush Polymer Cells by Shtylev, G. F., Shishkin, I. Yu, Shershov, V. E., Kuznetsova, V. E., Kachulyak, D. A., Butvilovskaya, V. I., Levashova, A. I., Vasiliskov, V. A., Zasedateleva, O. A., Chudinov, A. V.

“…Objective: The biochip method allows microscale multiparametric analysis of macromolecular samples using a matrix of immobilized molecular probes. Selection of…”
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Synthesis of Fluorescent-Labeled Nucleotides for Labeling of Isothermal Amplification Products by Shershov, V. E., Lapa, S. A., Levashova, A. I., Shishkin, I. Yu, Shtylev, G. F., Shekalova, E. Yu, Vasiliskov, V. A., Zasedatelev, A. S., Kuznetsova, V. E., Chudinov, A. V.

“…Fluorescently labeled nucleotides that contain a zwitterionic indodicarbocyanine dye have been synthesized. The dye has been attached at the C5 position of the…”
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A DNA Biochip with Cells of Carboxylated Brush Polymers by Miftakhov, R. A., Ikonnikova, A. Yu, Vasiliskov, V. A., Lapa, S. A., Levashova, A. I., Kuznetsova, V. E., Shershov, V. E., Zasedatelev, A. S., Nasedkina, T. V., Chudinov, A. V.

“…A method for the photolithographic fabrication of biochips with hydrogel cells made of brush acrylic acid–acrylamide copolymers fixed by one end on the surface…”
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Development of Biological Microchips on an Aluminum Support with Cells Made of Brush Polymers by Shishkin, I. Yu, Shtylev, G. F., Barsky, V. E., Lapa, S. A., Zasedateleva, O. A., Kuznetsova, V. E., Shershov, V. E., Vasiliskov, V. A., Polyakov, S. A., Zasedatelev, A. S., Chudinov, A. V.

“…A method has been developed for manufacturing biological microchips on an aluminum substrate with hydrophilic cells from brush copolymers with the formation of…”
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Biochip with Agarose Microcells Containing Thermally Separable Primers by Zolotov, A. M., Miftakhov, R. A., Ikonnikova, A. Y., Lapa, S. A., Kuznetsova, V. E., Vasiliskov, V. A., Shershov, V. E., Zasedatelev, A. S., Nasedkina, T. V., Chudinov, A. V.

“…— A method has been developed for the immobilization of short DNA sequences in agarose cells fixed on the surface of a polymer substrate, with their subsequent…”
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Development of Multiplex RT-PCR with Immobilized Primers for Identification of Infectious Human Pneumonia Pathogens by Lapa, S. A., Miftakhov, R. A., Klochikhina, E. S., Ammur, Yu. I., Blagodatskikh, S. A., Shershov, V. E., Zasedatelev, A. S., Chudinov, A. V.

“…A prototype of a system for the detection of infectious human pneumonia pathogens based on multiplex solid-phase reverse transcription PCR (RT-PCR) was…”
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Comparative Study of Novel Fluorescent Cyanine Nucleotides: Hybridization Analysis of Labeled PCR Products Using a Biochip by Shershov, V. E., Lapa, S. A., Kuznetsova, V. E., Spitsyn, M. A., Guseinov, T. O., Polyakov, S. A., Stomahin, A. A., Zasedatelev, A. S., Chudinov, A. V.

“…This study investigated the synthesis and substrate properties of Cy5-labeled dUTP derivatives with different substituents, linkers between the dye unit and…”
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Effect of Spacers on DNA Probe Properties in Hybridization Analysis by Miftakhov, R. A., Lapa, S. A., Kuznetsova, V. E., Zolotov, A. M., Vasiliskov, V. A., Shershov, V. E., Surzhikov, S. A., Zasedatelev, A. S., Chudinov, A. V.

“…— A method of immobilizing oligonucleotides on the surface of a polyethylene terephthalate (PET) substrate has been developed. The effectiveness of the…”
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A Simultaneous Use of Cy5-Modified Derivatives of Deoxyuridine and Deoxycytidine in PCR by Lapa, S. A., Guseinov, T. O., Pavlov, A. S., Shershov, V. E., Kuznetsova, V. E., Zasedatelev, A. S., Chudinov, A. V.

“…Particular features of the simultaneous incorporation into the growing DNA chain of modified Cy5-deoxyuridines (Cy5-dU) and deoxycytidines (Cy5-dC) and Taq DNA…”
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The Use of Thermal Dissociation for Selection of DNA Aptamers by Lapa, S. A., Shershov, V. E., Krasnov, G. S., Volkova, O. S., Kuznetsova, V. E., Radko, S. P., Zasedatelev, A. S., Chudinov, A. V.

“…A method of thermal dissociation of DNA–protein conjugates was developed for the selection of aptamers to human alpha-fetoprotein. The method is based on the…”
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Simultaneous Incorporation of Modified dU and dC Derivatives in the Growing DNA Chain Using PEX and PCR by Chudinov, A. V., Shershov, V. E., Pavlov, A. S., Volkova, O. S., Kuznetsova, V. E., Zasedatelev, A. S., Lapa, S. A.

“…The substrate efficiency of modified derivatives of 2'-deoxyuridine-(mod-dUTP) and 2'-deoxycytidine-5'-triphosphates (mod-dCTP) was studied in the regime of…”
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Slippage of the Primer Strand in the Primer Extension Reaction with Modified 2'-Deoxyuridine Triphosphates by Vasiliskov, V. A., Shershov, V. E., Miftahov, R. A., Kuznetsova, V. E., Radko, S. P., Lisitsa, A. V., Lapa, S. A., Surzhikov, S. A., Timofeev, E. N., Zasedatelev, A. S., Chudinov, A. V.

“…An effect of primer strand slippage was observed in the primer extension reaction due to replacement of thymidine triphosphate for modified analogs of dUTP…”
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Preparation of Modified Combinatorial DNA Libraries via Emulsion PCR with Subsequent Strand Separation by Lapa, S. A., Romashova, K. S., Spitsyn, M. A., Shershov, V. E., Kuznetsova, V. E., Guseinov, T. O., Zasedateleva, O. A., Radko, S. P., Timofeev, E. N., Lisitsa, A. V., Chudinov, A. V.

“…— A modification of the enzymatic method for the preparation of combinatorial random DNA libraries, which combines amplification in isolated microvolumes with…”
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Enzymatic Preparation of Modified DNA: Study of the Kinetics by Real-Time PCR by Lapa, S. A., Pavlov, A. S., Kuznetsova, V. E., Shershov, V. E., Spitsyn, M. A., Guseinov, T. O., Radko, S. P., Zasedatelev, A. S., Lisitsa, A. V., Chudinov, A. V.

“…The effects of modified deoxyuridine triphosphates (mod-dUTPs) with different substituents at the C5 position of the pyrimidine cycle on the kinetics of PCR…”
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Specificities of multi-primer polymerase chain reaction optimization for the detection of infectious pneumonia agents in human by Klochikhina, E. S., Shershov, V. E., Kuznetsova, V. E., Lapa, S. A., Chudinov, A. V.

“…Objectives. The objectives of this work are the development of a multi-primer system based on the polymerase chain reaction (PCR) aimed at the simultaneous…”
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Emulsion PCR Amplification of DNA Libraries with Degenerate Central Regions for Aptamer Selection by Kuznetsova, V. E., Shershov, V. E., Miftahov, R. A., Lapa, S. A., Zasedatelev, A. S., Timofeev, E. N., Chudinov, A. V.

“…For emulsion PCR amplification of oligonucleotide combinatorial libraries the conditions for the stable generation of water-in-oil emulsions with drops of…”
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