Search Results - "Shepherd, Jack"

SerraNA: a program to determine nucleic acids elasticity from simulation data by Velasco-Berrelleza, Victor, Burman, Matthew, Shepherd, Jack W, Leake, Mark C, Golestanian, Ramin, Noy, Agnes

“…The resistance of DNA to stretch, twist and bend is broadly well estimated by experiments and is important for gene regulation and chromosome packing. However,…”
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The End Restraint Method for Mechanically Perturbing Nucleic Acids In Silico by Shepherd, Jack W, Leake, Mark C

“…Far from being a passive information store, the genome is a mechanically dynamic and diverse system in which torsion and tension fluctuate and combine to…”
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Correlating fluorescence microscopy, optical and magnetic tweezers to study single chiral biopolymers such as DNA by Shepherd, Jack W., Guilbaud, Sebastien, Zhou, Zhaokun, Howard, Jamieson A. L., Burman, Matthew, Schaefer, Charley, Kerrigan, Adam, Steele-King, Clare, Noy, Agnes, Leake, Mark C.

“…Biopolymer topology is critical for determining interactions inside cell environments, exemplified by DNA where its response to mechanical perturbation is as…”
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RecA and RecB: probing complexes of DNA repair proteins with mitomycin C in live Escherichia coli with single-molecule sensitivity by Payne-Dwyer, Alex L, Syeda, Aisha H, Shepherd, Jack W, Frame, Lewis, Leake, Mark C

“…The RecA protein and RecBCD complex are key bacterial components for the maintenance and repair of DNA. RecBCD is a helicase-nuclease that uses homologous…”
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The emergence of sequence-dependent structural motifs in stretched, torsionally constrained DNA by Shepherd, Jack W, Greenall, Robert J, Probert, Matt I J, Noy, Agnes, Leake, Mark C

“…The double-helical structure of DNA results from canonical base pairing and stacking interactions. However, variations from steady-state conformations…”
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Molecular crowding in single eukaryotic cells: Using cell environment biosensing and single-molecule optical microscopy to probe dependence on extracellular ionic strength, local glucose conditions, and sensor copy number by Shepherd, Jack W, Lecinski, Sarah, Wragg, Jasmine, Shashkova, Sviatlana, MacDonald, Chris, Leake, Mark C

“…•FRET-based crowding sensor used in yeast in high- and low-stress conditions.•Confocal microscopy used to generate subcellular crowding maps.•Yeast experience…”
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Correlating viscosity and molecular crowding with fluorescent nanobeads and molecular probes: in vitro and in vivo by Lecinski, Sarah, Shepherd, Jack W, Bunting, Kate, Dresser, Lara, Quinn, Steven D, MacDonald, Chris, Leake, Mark C

“…In eukaryotes, intracellular physico-chemical properties like macromolecular crowding and cytoplasmic viscoelasticity influence key processes such as metabolic…”
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Elucidating the Role of Topological Constraint on the Structure of Overstretched DNA Using Fluorescence Polarization Microscopy by Backer, Adam S, King, Graeme A, Biebricher, Andreas S, Shepherd, Jack W, Noy, Agnes, Leake, Mark C, Heller, Iddo, Wuite, Gijs J. L, Peterman, Erwin J. G

“…The combination of DNA force spectroscopy and polarization microscopy of fluorescent DNA intercalator dyes can provide valuable insights into the structure of…”
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PySTACHIO: Python Single-molecule TrAcking stoiCHiometry Intensity and simulatiOn, a flexible, extensible, beginner-friendly and optimized program for analysis of single-molecule microscopy data by Shepherd, Jack W., Higgins, Ed J., Wollman, Adam J.M., Leake, Mark C.

“…[Display omitted] •We present PySTACHIO, a refined version of our spot tracking algorithm.•We demonstrate highly improved performance over previous MATLAB…”
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Combining single-molecule super-resolved localization microscopy with fluorescence polarization imaging to study cellular processes by Shepherd, Jack W, Payne-Dwyer, Alex L, Lee, Ji-Eun, Syeda, Aisha, Leake, Mark C

“…Abstract Super-resolution microscopy has catalyzed valuable insights into the sub-cellular, mechanistic details of many different biological processes across a…”
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Crowding-induced morphological changes in synthetic lipid vesicles determined using smFRET by Quinn, Steven D, Dresser, Lara, Graham, Sarah, Conteduca, Donato, Shepherd, Jack, Leake, Mark C

“…Lipid vesicles are valuable mesoscale molecular confinement vessels for studying membrane mechanics and lipid-protein interactions, and they have found utility…”
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Tourism in Oppressed Destinations: Political responsibility and the prospect of oppression offsetting by Shepherd, Jack

“…•The challenges of political responsibility in tourism are discussed.•Current political consumerist approaches are problematised.•The novel concept of…”
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The Effect of Lithium on the Budding Yeast Saccharomyces cerevisiae upon Stress Adaptation by Reith, Patrick, Braam, Svenja, Welkenhuysen, Niek, Lecinski, Sarah, Shepherd, Jack, MacDonald, Chris, Leake, Mark C, Hohmann, Stefan, Shashkova, Sviatlana, Cvijovic, Marija

“…Lithium salts are used in the treatment of mood disorders, cancer, and Alzheimer's disease. It has been shown to prolong life span in several phyla; however,…”
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Investigating molecular crowding during cell division and hyperosmotic stress in budding yeast with FRET by Lecinski, Sarah, Shepherd, Jack W, Frame, Lewis, Hayton, Imogen, MacDonald, Chris, Leake, Mark C

“…Cell division, aging, and stress recovery triggers spatial reorganization of cellular components in the cytoplasm, including membrane bound organelles, with…”
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Exploring a unifying approach to peacebuilding through tourism: Abraham and Israel/Palestine by Shepherd, Jack

“…This work emerges from the encouragement of peace studies scholars to seek out commonalities that can unite rival sides in a conflict. Based on this call, I…”
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'I'm not your toy': rejecting a tourism boycott by Shepherd, Jack

“…As tourists become more politically conscious, the number of boycotts grows. Despite their proliferation, tourism boycotts rarely succeed. This study explores…”
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Providing counter-narratives: the positive role of hostels in the Israeli-Palestinian context by Shepherd, Jack, Laven, Daniel

“…Previous research suggests that tourism has a role to play in challenging the destructive dominant narratives of the Israeli-Palestinian context - discourses…”
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Useful funds, disappointing framework: tourism stakeholder experiences of INTERREG by Shepherd, Jack, Ioannides, Dimitri

“…Cross-border tourism cooperation is a fruitful form of engagement between borderland destinations, helping them boost their profile and minimise problems…”
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Autoethnographic journeys through contested spaces by Shepherd, Jack, Laven, Daniel, Shamma, Linda

“…Stemming from a frustration with the rigidity of categories within tourism studies and their associated violence of finalization and narrative entrapment, this…”
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The Impact of COVID-19 on UK University Students: Understanding the Interconnection of Issues Experienced during Lockdown by McGivern, Paul, Shepherd, Jack

“…The pandemic profoundly disrupted university students' lives. Many students have parenting and/or caring responsibilities and work part-time jobs…”
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