Stability and Transformations of bis-PNA/DNA Triplex Structural Isomers

Structurally isomeric complexes formed between homopyrimidine bis-PNAs (T 2 JT 2 JT 4 -linker-T 4 CT 2 CT 2 ) and single- and double-stranded DNA targets were investigated. These complexes are triplexes designated S1, S2 and S3 in order of increased mobility by polyacrylamide gel electrophoresis. It...

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Bibliographic Details
Published in:	Journal of biomolecular structure & dynamics Vol. 21; no. 4; pp. 503 - 512
Main Authors:	Krupnik, Olga V., Fadeeva, Natalia V., Kvitko, Nina P., Shepelev, Valerii A., Nielsen, Peter E., Lazurkin, Yurii S.
Format:	Journal Article
Language:	English
Published:	England Taylor & Francis Group 01-02-2004
Subjects:	Autoradiography DNA - chemistry DNA - metabolism Isomerism Kinetics Lysine - chemistry Lysine - metabolism Models, Molecular Nucleic Acid Conformation Peptide Nucleic Acids - chemistry Peptide Nucleic Acids - metabolism
Online Access:	Get full text
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Summary:	Structurally isomeric complexes formed between homopyrimidine bis-PNAs (T 2 JT 2 JT 4 -linker-T 4 CT 2 CT 2 ) and single- and double-stranded DNA targets were investigated. These complexes are triplexes designated S1, S2 and S3 in order of increased mobility by polyacrylamide gel electrophoresis. It is shown that the S3 isomer is formed only on double-stranded DNA and possesses highest stability. Isomers S2 and S1 are formed upon binding of bis-PNA to double-stranded as well as to single-stranded DNA. It was found that the stability of the isomer S1 increases dramatically in the presence of excess single-stranded oligonucleotide complementary to the bis-PNA. The structure of the stabilized S1 isomer is proposed to consist of two bis-PNA/DNA triplexes. The relationship between the yield of the isomer S1 formed on single-stranded DNA and the bis-PNA concentration was investigated and a kinetic model of the formation of S1 is presented.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0739-1102 1538-0254
DOI:	10.1080/07391102.2004.10506944