Search Results - "Shepard, Scot R."
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Initial purification of recombinant botulinum neurotoxin fragments for pharmaceutical production using hydrophobic charge induction chromatography
Published in Journal of Chromatography A (05-04-2002)“…Initial purification of two serotypic variants of recombinant botulinum neurotoxin toxin heavy chain fragment [rBoNT(H c)], produced intracellularly in the…”
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2
Discoloration of ceramic hydroxyapatite used for protein chromatography
Published in Journal of Chromatography A (01-09-2000)“…Hydroxyapatite chromatography was used to purify a recombinant human protein at preparative (400 g) scale. The hydroxyapatite column became progressively…”
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Recovery of intracellular recombinant proteins from the yeast Pichia pastoris by cell permeabilization
Published in Journal of biotechnology (23-10-2002)“…A cell permeabilization method for the release of intracellular proteins from microbial cells was developed. The method was applied to the recovery of…”
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Identifying and modulating disulfide formation in the biopharmaceutical production of a recombinant protein vaccine candidate
Published in Journal of biotechnology (15-08-2003)“…Structural conversion of the serotype A recombinant botulinum neurotoxin heavy chain fragment (rBoNTA(H c)) produced intracellularly in Pichia pastoris yeast…”
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Large-Scale Purification of Recombinant Human Angiostatin
Published in Protein expression and purification (01-11-2000)“…A process for the purification of recombinant human angiostatin (rhAngiostatin), produced by Pichia pastoris fermentation operated at the 2000-L scale, is…”
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Routine manufacture of recombinant proteins using expanded bed adsorption chromatography
Published in Bioseparation (2001)“…Two different recombinant human proteins were purified directly from Pichia pastoris whole cell fermentation broth, containing 30-44% biomass (wet weight…”
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Monoclonal antibodies specific to sailfish serum albumin: development of an assay for the identification of fish species in the field
Published in Hybridoma (01-06-1992)“…Balb/c mice were immunized with albumin purified from sailfish (Istiophorus albicans) serum. Hybridomas were produced and screened by ELISA for reactivity with…”
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