Search Results - "Shende, Niraj"

Evaluation of a sensitive GC–MS method to detect polysorbate 80 in vaccine preparation by Shende, Niraj, Karale, Abhijeet, Bhagade, Sudhakar, Gulhane, Ashishkumar, Bore, Prashant, Marathe, Preeti, Chakraborty, Sourish, Mallya, Asha D., Dhere, Rajeev M.

“…•Transesterification of polysorbate 80 results in the formation of oleic acid methyl ester that can be analyzed by GC–MS.•The GC–MS technique was more…”
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Evaluation of GC-MS for identification and characterization of pneumococcal serotype 24A, 24B, and 24F capsular polysaccharide by Shende, Niraj, Karale, Abhijeet, Deshpande, Hrishikesh, Belapurkar, Hrushikesh, Gulhane, Ashish, Bhagade, Sudhakar, Bore, Prashant, Soni, Dipen, Marathe, Preeti, Patni, Sushil, Dhere, Rajeev, Mallya, Asha

“…Analysis of pneumococcal polysaccharides (PnPs) has been an arduous task, especially in similar serotypes. Pneumococci invades the host immune response by…”
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Evaluation of structural modification induced activation of pneumococcal polysaccharide by GC-MS for the conjugate vaccine by Shende, Niraj, Karale, Abhijeet, Bore, Prashant, Bhagade, Sudhakar, Gulhane, Ashishkumar, Mallya, Asha D., Dhere, Rajeev M.

“…Polysaccharide (Ps) activation evaluation is an imperative quality attribute in a conjugate vaccine. Pneumococcal polysaccharide (PnPs) serotypes 5, 6B, 14,…”
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Quantitation of endotoxin by gas chromatography-mass spectrometry in Neisseria meningitidis serogroups A, C, W, Y and X during polysaccharide purification used in conjugate vaccine by Shende, Niraj, Karale, Abhijeet, Marne, Kishor, Deshpande, Hrishikesh, Belapurkar, Hrushikesh, Mallya, Asha D., Dhere, Rajeev M.

“…•Transesterification and acetylation of lipopolysaccharide in meningococcal PS results in 3-O-acetyl tetradecanoic acid.•3-hydroxyl fatty acid methyl ester…”
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Serodiagnosis of tuberculosis using two ELISA systems by Banerjee, Swati, Gupta, Sonika, Shende, Niraj, Kumar, Satish, Harinath, Bhaskar C

“…Serodiagnosis by ELISA has been widely explored over the years, in the diagnosis of tuberculosis. Two ELISA systems were evaluated for detection of…”
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Transferrin binding protein-B from Neisseria meningitidis C as a novel carrier protein in glycoconjugate preparation: an in silico approach by Karale, Abhijeet, Lokhande, Kiran Bharat, Shende, Niraj, Swamy, K. V., Dhere, Rajeev, Nawani, Neelu, Mallya, Asha

“…The linking of polysaccharide in glycoconjugate vaccine with carrier protein is an imperative step to develop a strong memory response. The excessive use of…”
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Quantitation of residual sodium dodecyl sulfate in meningococcal polysaccharide by gas chromatography-mass spectrometry by Shende, Niraj, Karale, Abhijeet, Marathe, Preeti, Chakraborty, Sourish, Mallya, Asha D., Dhere, Rajeev M.

“…Sodium dodecyl sulfate (SDS) is a commonly used surfactant in protein solubilization and also during the polysaccharide purification. A GC-MS method has been…”
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Analysis of SEVA TB ES-31 antigen specific immunoglobulins IgM, IgA and IgG in sera of sputum and culture positive pulmonary tuberculosis by Gupta, Sonika, Shende, Niraj, Banerjee, Swati, Kumar, Satish, Reddy, M V R, Harinath, Bhaskar C

“…Tuberculosis remains major health problem in India and developing countries Immunodiagnosis has important role in screening, diagnosis and management of…”
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A cocktail of affinity-purified antibodies reactive with diagnostically useful mycobacterial antigens ES-31, ES-43, and EST-6 for detecting the presence of Mycobacterium tuberculosis by Harinath, Bhaskar Chinnaiah, Kumar, Satish, Roy, Santa Saha, Hirudkar, Suvarna, Upadhye, Vijay, Shende, Niraj

“…A cocktail of affinity-purified antibodies against diagnostically useful Mycobacterium tuberculosis H 37Ra excretory–secretory protein antigens ES-31, ES-43,…”
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Detection of in vitro and in vivo released antigens of diagnostic interest in Mycobacterium tuberculosis by immunoblotting by Shende, Niraj, Gupt, Sonika, Upadhye, Vijay, Kumar, Satish, Harinath, Bhaskar C

“…Identification of in vitro and in vivo released mycobacterial antigens are of considerable interest in diagnosis of Mycobacterium tuberculosis. Isolation of in…”
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Isolation of Mycobacterium tuberculosis protein antigens ES-3 1, ES-43 and EST-6 of diagnostic interest from tubercle bacilli by affinity chromatography by Upadhye, Vijay, Saha-Roy, Santa, Shende, Niraj, Kumar, Satish, Harinath, B C

“…Immunodiagnostically useful M. tuberculosis H37Ra protein antigens ES-31, ES-43 and EST-6 were isolated from detergent soluble sonicate (DSS) antigen using…”
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Effectivity of crude versus purified mycobacterial secretory proteins as immunogen for optimum antibody production by Saha-Roy, Santa, Shende, Niraj, Kumar, Satish, Harinath, B C

“…Monospecific antibodies have been successfully utilized in antigen detection, which is better indicator of active infection. Mycobacterium tuberculosis…”
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Detection of antibodies to a cocktail of mycobacterial excretory–secretory antigens in tuberculosis by ELISA and immunoblotting by Gupta, Sonika, Shende, Niraj, Kumar, Satish, Harinath, B. C.

“…The seroreactivity of a cocktail of purified mycobacterial excretory–secretory (ES) antigens ES-31, ES-41 and ES43 was assessed by ELISA and immunoblotting in…”
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IgG subclass antibody response to mycobacterial serine protease at different stages of pulmonary tuberculosis by Gupta, Sonika, Shende, Niraj, Bhatia, Amarjeet Singh, Kumar, Satish, Harinath, Baskar C

“…Tuberculosis (TB) is a chronic bacterial infection caused by M. Tuberculosis. Studies of antibody response in TB have focussed mainly on their usefulness as a…”
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Detection of antigen and antibody in childhood tuberculous meningitis by BERA, Swati, SHENDE, Niraj, KUMAR, Satish, HARINATH, B. C

“…Mycobacterium tuberculosis excretory secretory 31 kDa, a serine protease antigen (M. tb ES-31), prepared from Mycobacterium tuberculosis H37Ra culture medium…”
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Isolation and analysis of circulating tuberculous antigens in Mycobacterium tuberculosis by Shende, Niraj, Gupta, Sonika, Upadhye, Vijay, Kumar, Satish, Harinath, Bhaskar C

“…Serological techniques like enzyme linked immunosorbent assay (ELISA) and immunoblotting are useful for detection of mycobacterial antigens of diagnostic…”
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Levels of antibody, free antigen and immune-complexed antigen by ELISA in different grades of sputum positive patients of pulmonary tuberculosis by Shende, Niraj, Gupta, Sonika, Kumar, Satish, Harinath, B C

“…The ES-31 (31 kDa protein) antigen was isolated from culture filtrate of Mycobacterium tuberculosis H37Ra and was shown to have potential in immunodiagnosis of…”
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