Anticancer Activity of Polysaccharides Produced from Glycerol and Crude Glycerol by an Endophytic Fungus Chaetomium globosum CGMCC 6882 on Human Lung Cancer A549 Cells

Two polysaccharides were produced by CGMCC 6882 from glycerol (GCP-1) and crude glycerol (GCP-2). Chemical characteristics results showed GCP-1 and GCP-2 were similar polysaccharides, but the molecular weights of GCP-1 and GCP-2 were 5.340 × 10⁴ Da and 3.105 × 10⁴ Da, respectively. Viabilities of A5...

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Bibliographic Details
Published in:	Biomolecules (Basel, Switzerland) Vol. 8; no. 4; p. 171
Main Authors:	Wang, Zichao, Chen, Peizhang, Tao, Ning, Zhang, Huiru, Li, Ruifang, Zhan, Xiaobei, Wang, Fuzhuan, Shen, Yingben
Format:	Journal Article
Language:	English
Published:	Switzerland MDPI AG 11-12-2018 MDPI
Subjects:	A549 Cells anticancer activity Antitumor activity Apoptosis Apoptosis - drug effects Bcl-2 protein Biodiesel fuels Biofuels By products Cancer Carbon dioxide Caspase-3 Caspase-9 Chaetomium - chemistry Chaetomium globosum Chaetomium globosum CGMCC 6882 Chromatography crude glycerol Dehydrogenases Endophytes Endophytes - chemistry Fermentation Flow cytometry Fungi Gene Expression Regulation, Neoplastic - drug effects Glycerol Glycerol - chemistry Herbal medicine Humans Lung cancer Lung Neoplasms - drug therapy Lung Neoplasms - pathology Molecular weight Neoplasm Proteins - genetics NMR Nuclear magnetic resonance Polymerase chain reaction polysaccharide Polysaccharides Polysaccharides - chemistry Polysaccharides - pharmacology Sensors Spectrum analysis Tissue inhibitor of metalloproteinase 1 Tissue inhibitor of metalloproteinase 2 United States > US Germany crude glycerol anticancer activity Chaetomium globosum CGMCC 6882 polysaccharide glycerol
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Summary:	Two polysaccharides were produced by CGMCC 6882 from glycerol (GCP-1) and crude glycerol (GCP-2). Chemical characteristics results showed GCP-1 and GCP-2 were similar polysaccharides, but the molecular weights of GCP-1 and GCP-2 were 5.340 × 10⁴ Da and 3.105 × 10⁴ Da, respectively. Viabilities of A549 cells after treatment with GCP-1 and GCP-2 were 49% and 39% compared to the control group. Meanwhile, flow cytometry results indicated that GCP-1 and GCP-2 could induce 17.79% and 24.28% of A549 cells to apoptosis with 200 μg/mL concentration treated for 24 h. RT-PCR results suggested that GCP-1 and GCP-2 could be used as potential and effective apoptosis inducers on A549 cells by increasing , , , , expression and decreasing expression. This research provided an innovative approach to using a byproduct of biodiesel production (crude glycerol) to produce polysaccharides of potential medicinal benefit.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	2218-273X 2218-273X
DOI:	10.3390/biom8040171