Overexpression of MtIPT gene enhanced drought tolerance and delayed leaf senescence of creeping bentgrass (Agrostis stolonifera L.)

Isopentenyltransferases (IPT) serve as crucial rate-limiting enzyme in cytokinin synthesis, playing a vital role in plant growth, development, and resistance to abiotic stress. Compared to the wild type, transgenic creeping bentgrass exhibited a slower growth rate, heightened drought tolerance, and...

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Published in:	BMC plant biology Vol. 24; no. 1; pp. 734 - 15
Main Authors:	Ai, Ye, Chen, Yinglong, Wang, Ning, Li, Jiaxing, Liu, Jinnan, Shen, Liangying, Sun, Xinbo, Han, Liebao, Chao, Yuehui
Format:	Journal Article
Language:	English
Published:	England BioMed Central Ltd 01-08-2024 BioMed Central BMC
Subjects:	Abiotic stress Adenosine Agrostis - genetics Agrostis - metabolism Agrostis - physiology Alkyl and Aryl Transferases - genetics Alkyl and Aryl Transferases - metabolism Analysis Anthocyanins Antioxidants Auxin Bioflavonoids Biosynthesis Cellular signal transduction China Chlorophyll Cloning Creeping bentgrass Cytokinins Cytokinins - metabolism Drought Drought Resistance Drought tolerance Droughts Enzymes Flavones Flavonoids Gene expression Gene Expression Regulation, Plant Genes Genes, Plant Genetic engineering Genetically modified plants Influence Leaf senescence Leaves Microscopy MtIPT gene Oxidation Oxidation-reduction potential Period 1 protein Photosynthesis Photosynthesis - genetics Plant growth Plant Growth Regulators - metabolism Plant hormones Plant Leaves - genetics Plant Leaves - physiology Plant Proteins - genetics Plant Proteins - metabolism Plant Senescence - genetics Plants (botany) Plants, Genetically Modified - genetics Polyethylene glycol RNA Senescence Signal transduction Stress, Physiological - genetics Transgenic plants Waxes China Creeping bentgrass Leaf senescence MtIPT gene Drought tolerance Plant hormones
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Summary:	Isopentenyltransferases (IPT) serve as crucial rate-limiting enzyme in cytokinin synthesis, playing a vital role in plant growth, development, and resistance to abiotic stress. Compared to the wild type, transgenic creeping bentgrass exhibited a slower growth rate, heightened drought tolerance, and improved shade tolerance attributed to delayed leaf senescence. Additionally, transgenic plants showed significant increases in antioxidant enzyme levels, chlorophyll content, and soluble sugars. Importantly, this study uncovered that overexpression of the MtIPT gene not only significantly enhanced cytokinin and auxin content but also influenced brassinosteroid level. RNA-seq analysis revealed that differentially expressed genes (DEGs) between transgenic and wild type plants were closely associated with plant hormone signal transduction, steroid biosynthesis, photosynthesis, flavonoid biosynthesis, carotenoid biosynthesis, anthocyanin biosynthesis, oxidation-reduction process, cytokinin metabolism, and wax biosynthesis. And numerous DEGs related to growth, development, and stress tolerance were identified, including cytokinin signal transduction genes (CRE1, B-ARR), antioxidase-related genes (APX2, PEX11, PER1), Photosynthesis-related genes (ATPF1A, PSBQ, PETF), flavonoid synthesis genes (F3H, C12RT1, DFR), wax synthesis gene (MAH1), senescence-associated gene (SAG20), among others. These findings suggest that the MtIPT gene acts as a negative regulator of plant growth and development, while also playing a crucial role in the plant's response to abiotic stress.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1471-2229 1471-2229
DOI:	10.1186/s12870-024-05442-5