Catalase immobilized-radiation grafted functional cellulose matrix: A novel biocatalytic system

Catalase immobilized-radiation grafted functional cellulose matrix: A novel biocatalytic system

[Display omitted] •One step-radiation grafting process to prepare epoxy functionalized cellulose.•Catalase was covalently immobilized onto grafted cellulose via one step process.•Immobilized catalase was used for multiple cycles with no substantial activity loss.•Immobilized catalase showed higher t...

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Bibliographic Details
Published in:Journal of molecular catalysis. B, Enzymatic Vol. 133; pp. S172 - S178
Main Authors: Misra, Nilanjal, Goel, N.K., Shelkar, S.A., Varshney, L., Kumar, Virendra
Format: Journal Article
Language:English
Published: Elsevier B.V 01-11-2016
Subjects:
Catalase
Cellulose
Enzyme immobilization
Gamma radiation
Radiation grafting
Radiation grafting
Enzyme immobilization
Gamma radiation
Catalase
Cellulose
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Summary:[Display omitted] •One step-radiation grafting process to prepare epoxy functionalized cellulose.•Catalase was covalently immobilized onto grafted cellulose via one step process.•Immobilized catalase was used for multiple cycles with no substantial activity loss.•Immobilized catalase showed higher thermal stability compared to the free enzyme. The work highlights a unique, one step protocol for room temperature immobilization of enzyme catalase onto epoxy functionalised cellulose matrix developed via gamma radiation induced simultaneous irradiation grafting of Glycidylmethacrylate (GMA). Effect of grafting parameters, such as radiation dose, monomer concentration and solvent composition on the grafting yield, was studied in order to optimize the radiation grafting process. Poly(GMA)-g-cellulose matrices were characterized by grafting yield determination, FTIR, SEM and TGA techniques. The epoxy functionalized poly(GMA)-g-cellulose matrix was subsequently employed for covalent immobilization of an industrially relevant enzyme catalase. The catalytic activity of catalase-immobilized-poly(GMA)-g-cellulose was assayed by spectrophotometrically monitoring the enzymatic degradation of H2O2 at 240nm. Catalase-immobilized-poly(GMA)-g-cellulose was observed to be reusable for over 5 cycles within ten days. Catalase was observed to show improved activity at higher pH after immobilization. Thermal stability of Catalase-immobilized-poly(GMA)-g-cellulose was also enhanced in comparison to the free enzyme system.
ISSN:1381-1177
1873-3158
DOI:10.1016/j.molcatb.2017.01.001