Search Results - "Sheets, M F"

Time-dependent changes in kinetics of Na+ current in single canine cardiac Purkinje cells by Hanck, D A, Sheets, M F

“…The spontaneous hyperpolarizing shift in Na+ channel kinetics that occurs during a series of voltage-clamp recordings was characterized in single canine…”
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Extracellular divalent and trivalent cation effects on sodium current kinetics in single canine cardiac Purkinje cells by Hanck, D A, Sheets, M F

“…1. The effects of the extracellular divalent cations barium, calcium, cadmium, cobalt, magnesium, manganese, nickel and zinc and the trivalent cation lanthanum…”
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Mechanisms of extracellular divalent and trivalent cation block of the sodium current in canine cardiac Purkinje cells by Sheets, M F, Hanck, D A

“…1. Single canine cardiac Purkinje cells were internally perfused and voltage clamped with a large-bore perfusion pipette for measurement of sodium ionic…”
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Nonlinear relation between Vmax and INa in canine cardiac Purkinje cells by SHEETS, M. F, HANCK, D. A, FOZZARD, H. A

“…We studied the relation of the maximal upstroke velocity (Vmax) of action potentials to the peak sodium current (INa) under voltage clamp in single, internally…”
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Optimization of a mammalian expression system for the measurement of sodium channel gating currents by Sheets, M F, Kyle, J W, Krueger, S, Hanck, D A

“…We describe a new mammalian expression system that optimizes conditions for the measurement of Na channel gating currents (Ig). The small magnitude of Ig…”
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Alteration of the sodium current in cat cardiac ventricular myocytes during primary culture by Schackow, T E, Sheets, M F, Decker, R S, Ten Eick, R E

“…To determine the response of cardiac Na current (INa) in adult cardiac ventricular myocytes to culture, single isolated ventricular myocytes from…”
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Gating currents associated with Na channels in canine cardiac Purkinje cells by HANCK, D. A, SHEETS, M. F, FOZZARD, H. A

“…Gating currents (Ig) were recorded in single canine cardiac Purkinje cells at 10-12 degrees C. Ig characteristics corresponded closely to macroscopic INa…”
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The Na Channel Voltage Sensor Associated with Inactivation Is Localized to the External Charged Residues of Domain IV, S4 by Sheets, Michael F., Kyle, John W., Kallen, Roland G., Hanck, Dorothy A.

“…Site-3 toxins have been shown to inhibit a component of gating charge (33% of maximum gating charge, Q max) in native cardiac Na channels that has been…”
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Sodium current in voltage clamped internally perfused canine cardiac Purkinje cells by Makielski, J.C., Sheets, M.F., Hanck, D.A., January, C.T., Fozzard, H.A.

“…Study of the excitatory sodium current (INa) intact heart muscle has been hampered by the limitations of voltage clamp methods in multicellular preparations…”
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Open sodium channel properties of single canine cardiac Purkinje cells by Sheets, M.F., Scanley, B.E., Hanck, D.A., Makielski, J.C., Fozzard, H.A.

“…Open channel properties of canine cardiac Purkinje cell Na+ channels were studied with single channel cell-attached recording and with whole cell macroscopic…”
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Molecular action of lidocaine on the voltage sensors of sodium channels by Sheets, Michael F, Hanck, Dorothy A

“…Block of sodium ionic current by lidocaine is associated with alteration of the gating charge-voltage (Q-V) relationship characterized by a 38% reduction in…”
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Isolation and Characterization of Single Canine Cardiac Purkinje Cells by Sheets, Michael F, January, Craig T, Fozzard, Harry A

“…Single cardiac Purkinje cells should permit improved control of membrane potential during voltage clamp studies. We have developed a method for isolation of…”
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Cellular mechanism of action of cardiac glycosides by Fozzard, Harry A., Sheets, Michael F.

“…It has long been known that cardiac glycosides can inhibit the membrane sodium-potassium (Na+-K+) pump, raising intracellular Na+. However, at clinical…”
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Gating of skeletal and cardiac muscle sodium channels in mammalian cells by Sheets, Michael F., Hanck, Dorothy A.

“…Sodium channel ionic current ( I Na ) and gating current ( I g ) were compared for rat skeletal (rSkM1) and human heart Na + channels (hH1a) heterologously…”
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Use-dependent block of sodium current by ethmozin in voltage-clamped internally perfused canine cardiac Purkinje cells by Makielski, J C, Undrovinas, A I, Hanck, D A, Sheets, M F, Nesterenko, V V, Alpert, L A, Rosenshtraukh, L V, Fozzard, H A

“…Block of sodium current (INa) by ethmozin (moricizine), an antiarrhythmic drug, was investigated in isolated, voltage-clamped, canine cardiac Purkinje cells…”
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Lidocaine alters activation gating of cardiac Na channels by Hanck, D A, Makielski, J C, Sheets, M F

“…The class IB antiarrhythmic drug, lidocaine, interacts strongly with depolarized sodium (Na) channels, an action that is thought to underlie its clinical…”
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The role of the putative inactivation lid in sodium channel gating current immobilization by Sheets, M F, Kyle, J W, Hanck, D A

“…We investigated the contribution of the putative inactivation lid in voltage-gated sodium channels to gating charge immobilization (i.e., the slow return of…”
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Voltage-dependent open-state inactivation of cardiac sodium channels: gating current studies with Anthopleurin-A toxin by Sheets, M F, Hanck, D A

“…The gating charge and voltage dependence of the open state to the inactivated state (O-->I) transition was measured for the voltage-dependent mammalian cardiac…”
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Modification of the cell based assay for brevetoxins using human cardiac voltage dependent sodium channels expressed in HEK-293 cells by Fairey, Elizabeth R., Bottein Dechraoui, Marie-Yasmine, Sheets, Michael F., Ramsdell, John S.

“…Assays using living cells provide an effective means to generate activity measurements of toxins, especially in situations where the toxins are part of a…”
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Sodium channels in cardiac Purkinje cells by Fozzard, H A, Hanck, D A, Makielski, J C, Scanley, B E, Sheets, M F

“…Sodium (Na+) currents are responsible for excitation and conduction in most cardiac cells, but their study has been hampered by the lack of a satisfactory…”
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